Abstract
An LC/MS method has been developed for the simultaneous quantification of five flavonoids, i.e., mosloflavone, negletein, gardenin B, 5-methoxy-6,7-methylenedioxyflavone, and 5,6,7-trimethoxyflavone, in different ultrasound assisted solvent extracts of Actinocarya tibetica. The chromatographic separation was achieved on a Chromolith Speed ROD RP-18e column with gradient elution using methanol and 0.1% formic acid in water. The calibration curves of all five analytes showed good linearity (R2>0.991). Accuracy and precision were within the required limits. The developed method could serve as an effective method for QC of A. tibetica. The investigated compounds were determined simultaneously for the first time in A. tibetica or any other plant.
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Claims 4
Claim · model-written summary
The LC-MS/MS method gave good linearity (R2>0.991) for the calibration curves of all five flavonoids.
- Taxon
- Actinocarya tibeticaActinocarya tibeticae0
- Outcome
- linearitye1
- Value
- R2>0.991e1
Verbatim
e0“in different ultrasound assisted solvent extracts of Actinocarya tibetica”
e1“The calibration curves of all five analytes showed good linearity (R2>0.991)”
Claim · model-written summary
Accuracy and precision of the method were within the required limits.
Verbatim
e2“Accuracy and precision were within the required limits”
Claim · model-written summary
The developed method could serve as an effective quality control method for Actinocarya tibetica.
- Taxon
- A. tibeticaActinocarya tibeticae3
- Outcome
- QCe3
Verbatim
e3“The developed method could serve as an effective method for QC of A. tibetica”
Claim · model-written summary
The five investigated flavonoids were determined simultaneously for the first time in Actinocarya tibetica.
- Taxon
- A. tibeticaActinocarya tibeticae4
- Outcome
- determined simultaneouslye4
Verbatim
e4“The investigated compounds were determined simultaneously for the first time in A. tibetica or any other plant”
e5“simultaneous quantification of five flavonoids”