Abstract
A simple HPLC/DAD method for determination of aloin in complex matrices is proposed, using aloin A as a marker compound. The chromatographic separation was achieved on C18 HPLC column, mobile phase composed of acetonitrile and water (pH 2.6) in gradient mode, flow-rate 1.5 ml/min, with simultaneous UV detection at 295 nm for aloin, and 255 nm and 375 nm for flavonoids. For the method the obtained validation results are in satisfactory ranges. This method is successfully applied for analysis of aloin in Aloe barbadensis Mill. plant dried exudate and pharmaceuticals which besides this exudate contain mixture of different extracts from: Achillea Millefolium L., herba; Calendula officinalis L., flos; Cornux mas L., plant cortex; Cynodon dactylon L., rhizome; Hypericum perforatum L., herba; Inula helenium L., radix and Viscum album L., herba. It is shown that other present compounds such as flavonoids (quercetin, hyperozid and rutin) do not interfere in determination and they may be analyzed simultaneously. This method is proposed to be used in control of pharmaceuticals.
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Claims 3
Claim · model-written summary
The HPLC/DAD method for aloin gave validation results in satisfactory ranges.
Verbatim
e0“For the method the obtained validation results are in satisfactory ranges”
Claim · model-written summary
The method was successfully applied to analyse aloin in Aloe barbadensis dried exudate and in pharmaceuticals containing it.
Aloe veraOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This method is successfully applied for analysis of aloin in Aloe barbadensis Mill. plant dried exudate and pharmaceuticals”
Claim · model-written summary
Flavonoids (quercetin, hyperozid and rutin) did not interfere in the aloin determination and can be analysed simultaneously.
Verbatim
e0“other present compounds such as flavonoids (quercetin, hyperozid and rutin) do not interfere in determination and they may be analyzed simultaneously”