Paper · 1999 · ePublications@SCU (Southern Cross University)

Use of bifunctional alpha-amylase subtilisin inhibitor promoter sequence of barley to confer expression in seeds, WO/2000/018926, patent

Agnelo Furtado; Robert J Henry; Kenneth J. Scott

4
claims
8
verbatim quotes
0
citations
3
research areas
—
Not open access

Abstract

The present invention provides a novel promoter that is functional in the seeds of plants, in particular the endosperm and/or aleurone and/or the scutellum. Preferably, the isolated promoter sequence of the invention further modulates the expression of a structural gene in response to the phytohormones ABA and/or GA. Alternatively or in addition, the promoter sequence of the present invention is capable of being repressed or otherwise down-regulated in response to GA. The present invention further encompasses genetic constructs capable of expressing a structural gene operably under the control of the inventive promoter sequence, and transgenic plants carrying the genetic constructs. The promoter sequence of the present invention is particularly useful for modifying the seed traits of plants.

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Claims 4

Method / resourceobservedoriginal resultbasis n/a

Claim · model-written summary

The promoter is described as functional in seeds, including endosperm, aleurone and scutellum.

Organ / stage
endosperm and/or aleurone and/or the scutellume0
Outcome
promoter that is functional in the seeds of plantse0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“novel promoter that is functional in the seeds of plants, in particular the endosperm and/or aleurone and/or the scutellum”

✓ located verbatim in the source · abstract, characters 33–155 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 76–82 · show in text ↑
Observationobservedoriginal resultbasis n/a

Claim · model-written summary

The promoter can modulate structural gene expression in response to ABA or GA.

Treatment
ABA and/or GAe0
Gene
structural genee0
Outcome
expression of a structural genee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“promoter sequence of the invention further modulates the expression of a structural gene in response to the phytohormones ABA and/or GA”

✓ located verbatim in the source · abstract, characters 182–317 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 76–82 · show in text ↑
Intervention effectobservedoriginal resultbasis n/a

Claim · model-written summary

GA can repress or down-regulate the promoter.

Treatment
GAe0
Outcome
promoter sequencee0
Direction
down-regulatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“promoter sequence of the present invention is capable of being repressed or otherwise down-regulated in response to GA”

✓ located verbatim in the source · abstract, characters 353–471 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 76–82 · show in text ↑
Method / resourceobservedoriginal resultbasis n/a

Claim · model-written summary

Genetic constructs and transgenic plants carrying the promoter are provided.

Outcome
genetic constructs capable of expressing a structural genee0

Not reportedComparatorValue

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“genetic constructs capable of expressing a structural gene operably under the control of the inventive promoter sequence, and transgenic plants carrying the genetic constructs”

✓ located verbatim in the source · abstract, characters 515–690 · show in text ↑
t0

“barley”

✓ located verbatim in the source · title, characters 76–82 · show in text ↑