Abstract
Pectin is a class of acidic plant cell wall polysaccharides that comprises a major fraction of the Type I primary cell wall, typical of dicotyledonous species. Galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. The enzyme pectin acetylesterase (PAE) can hydrolyze acetyl esters in homogalacturonan regions of pectin. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties. In vivo acetylesterase activity in Arabidopsis root cell walls was detected with an apparent optimum pH of 6.5. The degree of acetylation of two-week-old Arabidopsis shoot and root cell walls were 10% and 6% respectively. Eleven putative genes have been identified in the Arabidopsis genome with sequence similarity to known PAE encoding genes. Atpae3 × Atpae6 double mutant line had 70% and 83% of wild type root tissue of fresh weight and dry weight respectively. This significant difference could be the result of structural changes of cell wall of the double mutant line, since no difference was observed of root length or number of lateral roots between the wild type and Atpae3 x Atpae6 double mutant line. FTIR analysis on PGA with two different degrees of acetylation showed a wave number peak of 1747 cm-1.
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Claims 8
Claim · model-written summary
Eleven putative pectin acetylesterase genes were identified in the Arabidopsis genome by sequence similarity.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Gene
- PAE encoding genese0
- Outcome
- putative genese0
- Value
- Elevene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“A putative pectin acetylesterase gene family in Arabidopsis thaliana”
e0“Eleven putative genes have been identified in the Arabidopsis genome with sequence similarity to known PAE encoding genes”
Claim · model-written summary
In vivo acetylesterase activity in Arabidopsis root cell walls had an apparent optimum at pH 6.5.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- root cell wallse1
- Outcome
- acetylesterase activitye1
- Value
- pH of 6.5e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“A putative pectin acetylesterase gene family in Arabidopsis thaliana”
e1“In vivo acetylesterase activity in Arabidopsis root cell walls was detected with an apparent optimum pH of 6.5”
Claim · model-written summary
The degree of acetylation of two-week-old Arabidopsis shoot cell walls was 10 percent.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- two-week-old Arabidopsis shoote2
- Outcome
- degree of acetylatione2
- Value
- 10%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“A putative pectin acetylesterase gene family in Arabidopsis thaliana”
e2“The degree of acetylation of two-week-old Arabidopsis shoot and root cell walls were 10% and 6% respectively”
Claim · model-written summary
The degree of acetylation of two-week-old Arabidopsis root cell walls was 6 percent.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- root cell wallse2
- Outcome
- degree of acetylatione2
- Value
- 6%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“A putative pectin acetylesterase gene family in Arabidopsis thaliana”
e2“The degree of acetylation of two-week-old Arabidopsis shoot and root cell walls were 10% and 6% respectively”
Claim · model-written summary
The Atpae3 x Atpae6 double mutant had 70 percent of wild-type root fresh weight.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- root tissuee3
- Treatment
- Atpae3 × Atpae6 double mutant linee3
- Outcome
- fresh weighte3
- Direction
- decreaseinferrede3
- Value
- 70%e3
- Comparator
- wild typee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“A putative pectin acetylesterase gene family in Arabidopsis thaliana”
e3“Atpae3 × Atpae6 double mutant line had 70% and 83% of wild type root tissue of fresh weight and dry weight respectively”
Claim · model-written summary
The Atpae3 x Atpae6 double mutant had 83 percent of wild-type root dry weight.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- root tissuee3
- Treatment
- Atpae3 × Atpae6 double mutant linee3
- Outcome
- dry weighte3
- Direction
- decreaseinferrede3
- Value
- 83%e3
- Comparator
- wild typee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“A putative pectin acetylesterase gene family in Arabidopsis thaliana”
e3“Atpae3 × Atpae6 double mutant line had 70% and 83% of wild type root tissue of fresh weight and dry weight respectively”
Claim · model-written summary
The Atpae3 x Atpae6 double mutant showed no difference from wild type in root length or number of lateral roots.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Atpae3 x Atpae6 double mutant linee4
- Outcome
- root length or number of lateral rootse4
- Comparator
- wild typee4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“A putative pectin acetylesterase gene family in Arabidopsis thaliana”
e4“no difference was observed of root length or number of lateral roots between the wild type and Atpae3 x Atpae6 double mutant line”
Claim · model-written summary
FTIR analysis of PGA with two degrees of acetylation showed a wave number peak at 1747 cm-1.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- PGAe5
- Outcome
- wave number peake5
- Value
- 1747 cm-1e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“A putative pectin acetylesterase gene family in Arabidopsis thaliana”
e5“FTIR analysis on PGA with two different degrees of acetylation showed a wave number peak of 1747 cm-1”