Abstract
The host range of Agrobacterium tumefaciens 1D1109, known to induce crown gall only on grapevine (Vitis spp.), was extended to include many plant species by transferring a tumor-inducing plasmid (pTi) from strain 1D1, a broad-host-range pathogen. The pTi plasmid was mobilized by the conjugative plasmid pRK2, which was inserted into 1D1 by mating with Escherichia coli J53(pRK2). The resulting transconjugants were screened for their ability to induce crown gall tumors on hosts other than grapevine by inoculation into sunflower. Transconjugants that were virulent on sunflower were then tested on 36 different host plants and compared with host-limited strain 1D1109 and the donor strain. Two transconjugants induced tumors on the same 28 plant species as those of the original plasmid donor 1D1(pRK2) (pTi). These results show that pRK2 promoted transfer of the pTi plasmid and suggest that the pTi plasmid rather than the A. tumefaciens chromosome determined the host range of the pathogen. Insertion of pRK2 alone did not extend the host range of strain 1D1109. Insertion of pS-a into A. tumefaciens 1D1 by mating with E. coli J53-1 (pS-a) resulted in the concomitant loss of pTi and virulence. There appears to be incompatibility between pTi and pS-a.
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Claims 8
Claim · model-written summary
Transfer of pTi extended strain 1D1109 host range beyond grapevine.
- Treatment
- transferring a tumor-inducing plasmid (pTi)e0
- Interactor
- Agrobacterium tumefaciens
Agrobacterium tumefaciense0pathogen - Outcome
- host rangee0
- Direction
- extendede0
- Comparator
- Agrobacterium tumefaciens 1D1109e0
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The host range of Agrobacterium tumefaciens 1D1109, known to induce crown gall only on grapevine (Vitis spp.), was extended to include many plant species by transferring a tumor-inducing plasmid (pTi) from strain 1D1”
Claim · model-written summary
Two transconjugants induced tumors on the same 28 plant species as the donor.
- Taxon
- sunflower
Helianthus annuuse1 - Interactor
- Agrobacterium tumefaciensAgrobacterium tumefacienst1pathogene0
- Outcome
- tumorse0
- Value
- 28 plant speciese0
- Comparator
- original plasmid donor 1D1(pRK2) (pTi)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two transconjugants induced tumors on the same 28 plant species as those of the original plasmid donor 1D1(pRK2) (pTi)”
t0“Agrobacterium tumefaciens”
t1“Agrobacterium tumefaciens”
e1“by inoculation into sunflower”
Claim · model-written summary
pRK2 promoted transfer of the pTi plasmid.
- Taxon
- sunflowerHelianthus annuuse1
- Interactor
- Agrobacterium tumefaciensAgrobacterium tumefacienst1pathogene0
- Gene
- pTi plasmide0
- Outcome
- transfer of the pTi plasmide0
- Direction
- promotede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pRK2 promoted transfer of the pTi plasmid”
t0“Agrobacterium tumefaciens”
t1“Agrobacterium tumefaciens”
e1“by inoculation into sunflower”
Claim · model-written summary
The authors infer that pTi rather than the chromosome determined host range.
- Taxon
- sunflowerHelianthus annuuse1
- Interactor
- A. tumefaciensAgrobacterium tumefaciense0pathogen
- Gene
- pTi plasmide0
- Outcome
- host range of the pathogene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pTi plasmid rather than the A. tumefaciens chromosome determined the host range of the pathogen”
t0“Agrobacterium tumefaciens”
t1“Agrobacterium tumefaciens”
e1“by inoculation into sunflower”
Claim · model-written summary
Insertion of pRK2 alone did not extend host range.
- Taxon
- sunflowerHelianthus annuuse1
- Treatment
- Insertion of pRK2 alonee0
- Interactor
- Agrobacterium tumefaciensAgrobacterium tumefacienst1pathogene0
- Outcome
- host range of strain 1D1109e0
- Direction
- did not extende0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Insertion of pRK2 alone did not extend the host range of strain 1D1109”
t0“Agrobacterium tumefaciens”
t1“Agrobacterium tumefaciens”
e1“by inoculation into sunflower”
Claim · model-written summary
Insertion of pS-a into strain 1D1 caused loss of pTi.
- Taxon
- sunflowerHelianthus annuuse1
- Treatment
- Insertion of pS-ae0
- Interactor
- A. tumefaciensAgrobacterium tumefaciense0pathogen
- Outcome
- pTie0
- Direction
- losse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Insertion of pS-a into A. tumefaciens 1D1 by mating with E. coli J53-1 (pS-a) resulted in the concomitant loss of pTi and virulence”
t0“Agrobacterium tumefaciens”
t1“Agrobacterium tumefaciens”
e1“by inoculation into sunflower”
Claim · model-written summary
Insertion of pS-a into strain 1D1 caused loss of virulence.
- Taxon
- sunflowerHelianthus annuuse1
- Treatment
- Insertion of pS-ae0
- Interactor
- A. tumefaciensAgrobacterium tumefaciense0pathogen
- Outcome
- virulencee0
- Direction
- losse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Insertion of pS-a into A. tumefaciens 1D1 by mating with E. coli J53-1 (pS-a) resulted in the concomitant loss of pTi and virulence”
t0“Agrobacterium tumefaciens”
t1“Agrobacterium tumefaciens”
e1“by inoculation into sunflower”
Claim · model-written summary
The authors infer incompatibility between pTi and pS-a.
- Taxon
- sunflowerHelianthus annuuse1
- Interactor
- Agrobacterium tumefaciensAgrobacterium tumefacienst1pathogene0
- Gene
- pTie0
- Outcome
- incompatibility between pTi and pS-ae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There appears to be incompatibility between pTi and pS-a”
t0“Agrobacterium tumefaciens”
t1“Agrobacterium tumefaciens”
e1“by inoculation into sunflower”