Abstract
Doubling the concentration of atmospheric CO2 often inhibits plant respiration, but the mechanistic basis of this effect is unknown. We investigated the direct effects of increasing the concentration of CO2 by 360 [mu]L L-1 above ambient on O2 uptake in isolated mitochondria from soybean (Glycine max L. cv Ransom) cotyledons. Increasing the CO2 concentration inhibited the oxidation of succinate, external NADH, and succinate and external NADH combined. The inhibition was greater when mitochondria were preincubated for 10 min in the presence of the elevated CO2 concentration prior to the measurement of O2 uptake. Elevated CO2 concentration inhibited the salicylhydroxamic acid-resistant cytochrome pathway, but had no direct effect on the cyanide-resistant alternative pathway. We also investigated the direct effects of elevated CO2 concentration on the activities of cytochrome c oxidase and succinate dehydrogenase (SDH) and found that the activity of both enzymes was inhibited. The kinetics of inhibition of cytochrome c oxidase were time-dependent. The level of SDH inhibition depended on the concentration of succinate in the reaction mixture. Direct inhibition of respiration by elevated CO2 in plants and intact tissues may be due at least in part to the inhibition of cytochrome c oxidase and SDH.
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Claims 8
Claim · model-written summary
Elevated CO2 inhibited oxidation of succinate, external NADH, and their combination.
- Taxon
- soybean
Glycine maxe1 - Treatment
- Increasing the CO2 concentratione0
- Outcome
- oxidation of succinate, external NADH, and succinate and external NADH combinede0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing the CO2 concentration inhibited the oxidation of succinate, external NADH, and succinate and external NADH combined.”
e1“soybean (Glycine max L. cv Ransom) cotyledons”
Claim · model-written summary
Preincubation for 10 minutes with elevated CO2 increased inhibition of oxygen uptake.
- Taxon
- soybeanGlycine maxe1
- Treatment
- preincubated for 10 min in the presence of the elevated CO2 concentratione0
- Outcome
- inhibitione0
- Direction
- greatere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inhibition was greater when mitochondria were preincubated for 10 min in the presence of the elevated CO2 concentration prior to the measurement of O2 uptake.”
e1“soybean (Glycine max L. cv Ransom) cotyledons”
Claim · model-written summary
Elevated CO2 inhibited the cytochrome respiratory pathway.
- Taxon
- soybeanGlycine maxe1
- Treatment
- Elevated CO2 concentratione0
- Outcome
- salicylhydroxamic acid-resistant cytochrome pathwaye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevated CO2 concentration inhibited the salicylhydroxamic acid-resistant cytochrome pathway, but had no direct effect on the cyanide-resistant alternative pathway.”
e1“soybean (Glycine max L. cv Ransom) cotyledons”
Claim · model-written summary
Elevated CO2 had no direct effect on the alternative respiratory pathway.
- Taxon
- soybeanGlycine maxe1
- Treatment
- Elevated CO2 concentratione0
- Outcome
- cyanide-resistant alternative pathwaye0
- Direction
- no direct effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevated CO2 concentration inhibited the salicylhydroxamic acid-resistant cytochrome pathway, but had no direct effect on the cyanide-resistant alternative pathway.”
e1“soybean (Glycine max L. cv Ransom) cotyledons”
Claim · model-written summary
Elevated CO2 inhibited cytochrome c oxidase activity.
- Taxon
- soybeanGlycine maxe2
- Treatment
- elevated CO2 concentratione1
- Gene
- cytochrome c oxidasee1
- Outcome
- cytochrome c oxidasee1
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the activity of both enzymes was inhibited.”
e1“elevated CO2 concentration on the activities of cytochrome c oxidase and succinate dehydrogenase (SDH)”
e2“soybean (Glycine max L. cv Ransom) cotyledons”
Claim · model-written summary
Elevated CO2 inhibited succinate dehydrogenase activity.
- Taxon
- soybeanGlycine maxe2
- Treatment
- elevated CO2 concentratione1
- Gene
- succinate dehydrogenasee1
- Outcome
- succinate dehydrogenasee1
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the activity of both enzymes was inhibited.”
e1“elevated CO2 concentration on the activities of cytochrome c oxidase and succinate dehydrogenase (SDH)”
e2“soybean (Glycine max L. cv Ransom) cotyledons”
Claim · model-written summary
Cytochrome c oxidase inhibition varied with time.
- Taxon
- soybeanGlycine maxe1
- Gene
- cytochrome c oxidasee0
- Outcome
- kinetics of inhibition of cytochrome c oxidasee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The kinetics of inhibition of cytochrome c oxidase were time-dependent.”
e1“soybean (Glycine max L. cv Ransom) cotyledons”
Claim · model-written summary
Succinate dehydrogenase inhibition depended on succinate concentration.
- Taxon
- soybeanGlycine maxe1
- Compound
- succinatee0
- Outcome
- level of SDH inhibitione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of SDH inhibition depended on the concentration of succinate in the reaction mixture.”
e1“soybean (Glycine max L. cv Ransom) cotyledons”
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See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.