Abstract
In vitro regeneration was performed with the aim of developing efficient callus and shoot regeneration from different explants of sunflower (Helianthus annuus L.). The 6 genotypes (RHA10, RHA 14, RHA15, PR6404, N Record 109/ Sanay 3-5, N Record 109/ Sera) were used as plant materials. The roots, hypocotyls and cotyledons were excised from 4 day-old seedlings and cultured on embryo induction medium (EIM) supplemented with Benzylaminopurine (BAP), Naphthaleneacetic acid (NAA) and Gibberellic acid (GA3). The experiments were kept in 18/6 hour light/dark photoperiod at 26± 2 ℃ for four weeks. The rates of callus and root organ formation on explants were 67%-100% and 7%-31% respectively, depending on the genotype. Root explants produced statistically high callus formation (2.6 score) compared to cotyledon (2.0 score) and hypocotyl explants (2.5 score) at all sunflower genotypes used. The highest shoot regeneration was obtained from RHA15 (7%) while PR6404 (100%) produced the highest callus formation.
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Claims 5
Claim · model-written summary
Callus formation rates on sunflower explants were 67%-100%, depending on genotype.
- Taxon
- sunflower
Helianthus annuuse2 - Organ / stage
- explantse1
- Outcome
- calluse1
- Value
- 67%-100%e1
- Qualifier
- depending on the genotypee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rates of callus and root organ formation on explants were 67%-100% and 7%-31% respectively, depending on the genotype”
e2“sunflower (Helianthus annuus L.)”
Claim · model-written summary
Root organ formation rates on sunflower explants were 7%-31%, depending on genotype.
- Taxon
- sunflowerHelianthus annuuse2
- Organ / stage
- explantse1
- Outcome
- root organ formatione1
- Value
- 7%-31%e1
- Qualifier
- depending on the genotypee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rates of callus and root organ formation on explants were 67%-100% and 7%-31% respectively, depending on the genotype”
e2“sunflower (Helianthus annuus L.)”
Claim · model-written summary
Root explants produced statistically higher callus formation than cotyledon and hypocotyl explants in all sunflower genotypes used.
- Taxon
- sunflowerHelianthus annuuse3
- Treatment
- Root explantse3
- Outcome
- callus formatione3
- Direction
- highe3
- Value
- 2.6 scoree3
- Qualifier
- compared to cotyledon (2.0 score) and hypocotyl explants (2.5 score)e3
- Comparator
- cotyledone3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Root explants produced statistically high callus formation (2.6 score) compared to cotyledon (2.0 score) and hypocotyl explants (2.5 score) at all sunflower genotypes used”
Claim · model-written summary
RHA15 gave the highest shoot regeneration, 7%, among the sunflower genotypes.
- Taxon
- sunflowerHelianthus annuuse2
- Material
- RHA15e4
- Outcome
- shoot regeneratione4
- Value
- 7%e4
- Qualifier
- higheste4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“sunflower (Helianthus annuus L.)”
e4“The highest shoot regeneration was obtained from RHA15 (7%)”
Claim · model-written summary
PR6404 gave the highest callus formation, 100%, among the sunflower genotypes.
- Taxon
- sunflowerHelianthus annuuse2
- Material
- PR6404e5
- Outcome
- callus formatione5
- Value
- 100%e5
- Qualifier
- higheste5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“sunflower (Helianthus annuus L.)”
e5“while PR6404 (100%) produced the highest callus formation”
The same questions in other papers
in vitro · growth · Helianthus annuus (common sunflower)Positive and negative results
21 papers in the same direction as this paper · 14 in the opposite direction
- positive
- negative
- no effect
32 papers more in this group
See all 36 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.