Abstract
Movement proteins (MPs) encoded by plant viruses interact with host proteins to facilitate or interfere with intra- and/or intercellular viral movement. Using yeast two-hybrid and bimolecular fluorescence complementation assays, we herein present in vivo evidence for the interaction between Alfalfa mosaic virus (AMV) MP and Arabidopsis Patellin 3 (atPATL3) and Patellin 6 (atPATL6), two proteins containing a Sec14 domain. Proteins with Sec14 domains are implicated in membrane trafficking, cytoskeleton dynamics, lipid metabolism and lipid-mediated regulatory functions. Interestingly, the overexpression of atPATL3 and/or atPATL6 interfered with the plasmodesmata targeting of AMV MP and correlated with reduced infection foci size. Consistently, the viral RNA levels increased in the single and double Arabidopsis knockout mutants for atPATL3 and atPATL6. Our results indicate that, in general, MP-PATL interactions interfere with the correct subcellular targeting of MP, thus rendering the intracellular transport of viral MP-containing complexes less efficient and diminishing cell-to-cell movement.
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Claims 5
Claim · model-written summary
Alfalfa mosaic virus movement protein interacts with Arabidopsis Patellin 3 and 6.
- Taxon
- Arabidopsis
Arabidopsise0 - Interactor
- Alfalfa mosaic virus
Alfamovirus AMVe0pathogen - Gene
- Patellin 3 (atPATL3) and Patellin 6 (atPATL6)e0
- Outcome
- interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“interaction between Alfalfa mosaic virus (AMV) MP and Arabidopsis Patellin 3 (atPATL3) and Patellin 6 (atPATL6)”
Claim · model-written summary
Patellin overexpression interfered with movement protein targeting to plasmodesmata.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- overexpression of atPATL3 and/or atPATL6e0
- Interactor
- A lfalfa mosaic virust0pathogen
- Gene
- atPATL3 and/or atPATL6e0
- Outcome
- plasmodesmata targeting of AMV MPe0
- Direction
- interferede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of atPATL3 and/or atPATL6 interfered with the plasmodesmata targeting of AMV MP”
t0“A lfalfa mosaic virus”
e1“interaction between Alfalfa mosaic virus (AMV) MP and Arabidopsis Patellin 3 (atPATL3) and Patellin 6 (atPATL6)”
Claim · model-written summary
Patellin overexpression correlated with smaller infection foci.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Patellins 3 and 6t0
- Interactor
- A lfalfa mosaic virust0pathogen
- Outcome
- infection foci sizee0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“correlated with reduced infection foci size”
t0“Patellins 3 and 6, two members of the P lant P atellin family, interact with the movement protein of A lfalfa mosaic virus”
e1“interaction between Alfalfa mosaic virus (AMV) MP and Arabidopsis Patellin 3 (atPATL3) and Patellin 6 (atPATL6)”
Claim · model-written summary
Viral RNA levels increased in Arabidopsis Patellin knockouts.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- single and double Arabidopsis knockout mutants for atPATL3 and atPATL6e0
- Interactor
- A lfalfa mosaic virust0pathogen
- Gene
- atPATL3 and atPATL6e0
- Outcome
- viral RNA levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“viral RNA levels increased in the single and double Arabidopsis knockout mutants for atPATL3 and atPATL6”
t0“A lfalfa mosaic virus”
Claim · model-written summary
The authors infer that MP-PATL interactions impair viral movement through altered movement protein targeting.
- Taxon
- ArabidopsisArabidopsise1
- Interactor
- A lfalfa mosaic virust0pathogen
- Gene
- Patellins 3 and 6t0
- Outcome
- cell-to-cell movemente0
- Direction
- diminishinge0
- Qualifier
- correct subcellular targeting of MPe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MP-PATL interactions interfere with the correct subcellular targeting of MP, thus rendering the intracellular transport of viral MP-containing complexes less efficient and diminishing cell-to-cell movement”
t0“Patellins 3 and 6, two members of the P lant P atellin family, interact with the movement protein of A lfalfa mosaic virus”
e1“interaction between Alfalfa mosaic virus (AMV) MP and Arabidopsis Patellin 3 (atPATL3) and Patellin 6 (atPATL6)”