Abstract
Peptides corresponding to the most conserved domain of eubacterial flagellin act as potent elicitors in cells of different plant species. In intact Arabidposis thaliana seedlings these peptides (flg22 and flg15) caused callose deposition, induction of genes coding for pathogenesis-related proteins and a strong inhibition of growth. Half-maximal growth inhibition occurred at peptide concentrations of approximately 100 nM. In contrast, peptides representing the corresponding flagellin domains of the plant-associated bacteria A. tumefaciens and R. meliloti were inactive even at concentrations of 10 microM. With the exception of Ws-0, all ecotypes of A. thaliana tested were sensitive to flg22. Crosses of Ws-0 with the sensitive ecotypes Col-0 and La-er, respectively, resulted in sensitive F1 seedlings. In the F2 generation of both crosses, sensitivity segregated as a single trait with markers of chromosome 5 and a ratio of 3:1. Dominance of the locus sensing flagellin, termed FLS-1, suggests that it encodes an element which is important for the perception of the flagellin signal.
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Claims 10
Claim · model-written summary
Flagellin domain peptides act as potent elicitors in cells of different plant species.
- Taxon
- plant speciese0
- Treatment
- Peptides corresponding to the most conserved domain of eubacterial flagelline0
- Outcome
- potent elicitorse0
Not reportedComparatorDirection
Verbatim
e0“Peptides corresponding to the most conserved domain of eubacterial flagellin act as potent elicitors in cells of different plant species”
Claim · model-written summary
flg22 and flg15 peptides caused callose deposition in intact Arabidopsis seedlings.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Organ / stage
- seedlingse1
- Treatment
- these peptides (flg22 and flg15)e1
- Outcome
- callose depositione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e1“In intact Arabidposis thaliana seedlings these peptides (flg22 and flg15) caused callose deposition, induction of genes coding for pathogenesis-related proteins and a strong inhibition of growth”
Claim · model-written summary
flg22 and flg15 peptides induced genes coding for pathogenesis-related proteins in intact Arabidopsis seedlings.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- seedlingse1
- Treatment
- these peptides (flg22 and flg15)e1
- Gene
- pathogenesis-related proteinse1
- Outcome
- induction of genes coding for pathogenesis-related proteinse1
- Direction
- inductione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e1“In intact Arabidposis thaliana seedlings these peptides (flg22 and flg15) caused callose deposition, induction of genes coding for pathogenesis-related proteins and a strong inhibition of growth”
Claim · model-written summary
flg22 and flg15 peptides strongly inhibited growth of intact Arabidopsis seedlings.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- seedlingse1
- Treatment
- these peptides (flg22 and flg15)e1
- Outcome
- growthe1
- Direction
- inhibitione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e1“In intact Arabidposis thaliana seedlings these peptides (flg22 and flg15) caused callose deposition, induction of genes coding for pathogenesis-related proteins and a strong inhibition of growth”
Claim · model-written summary
Half-maximal growth inhibition by peptides occurred at about 100 nM.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- peptide concentrationse2
- Outcome
- growth inhibitione2
- Direction
- Half-maximale2
- Value
- approximately 100 nMe2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e2“Half-maximal growth inhibition occurred at peptide concentrations of approximately 100 nM”
Claim · model-written summary
Peptides from the flagellin domains of A. tumefaciens and R. meliloti were inactive even at 10 microM, unlike flg22.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- peptides representing the corresponding flagellin domains of the plant-associated bacteria A. tumefaciens and R. melilotie3
- Interactor
- A. tumefaciense3other
- Second interactor
- R. melilotie3other
- Outcome
- inactivee3
- Value
- 10 microMe3
- Comparator
- flg22e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e3“peptides representing the corresponding flagellin domains of the plant-associated bacteria A. tumefaciens and R. meliloti were inactive even at concentrations of 10 microM”
e1“In intact Arabidposis thaliana seedlings these peptides (flg22 and flg15) caused callose deposition, induction of genes coding for pathogenesis-related proteins and a strong inhibition of growth”
Claim · model-written summary
All Arabidopsis ecotypes tested except Ws-0 were sensitive to flg22.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- ecotypese4
- Outcome
- sensitive to flg22e4
- Qualifier
- With the exception of Ws-0e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e4“With the exception of Ws-0, all ecotypes of A. thaliana tested were sensitive to flg22”
Claim · model-written summary
F1 seedlings from Ws-0 crosses with the sensitive ecotypes Col-0 and La-er were sensitive to flagellin.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- Col-0 and La-ere5
- Organ / stage
- F1 seedlingse5
- Outcome
- sensitivee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e5“Crosses of Ws-0 with the sensitive ecotypes Col-0 and La-er, respectively, resulted in sensitive F1 seedlings”
Claim · model-written summary
In F2 generations of both crosses, flagellin sensitivity segregated as a single trait with markers of chromosome 5 in a 3:1 ratio.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Organ / stage
- F2 generatione6
- Outcome
- sensitivity segregated as a single traite6
- Value
- 3:1e6
- Qualifier
- markers of chromosome 5e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e6“In the F2 generation of both crosses, sensitivity segregated as a single trait with markers of chromosome 5 and a ratio of 3:1”
Claim · model-written summary
The dominant FLS-1 locus likely encodes an element important for perception of the flagellin signal.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- FLS-1e7
- Outcome
- the perception of the flagellin signale7
- Qualifier
- Dominance of the locus sensing flagelline7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e7“Dominance of the locus sensing flagellin, termed FLS-1, suggests that it encodes an element which is important for the perception of the flagellin signal”