Abstract
The present communication deals with the various in-vitro techniques attempted to conserve the germplasm of banana. The major aspects included are rapid clonal propagation through meristem culture of several cultivars belonging to different genomic constitutions, their medium-term storage under minimal growth condition and finally somatic embryogenesis. Rapid in-vitro multiplication of banana has been achieved by culturing the explants in Murashige and Skoog's semi-solid basal medium supplemented with low concentration of indole acetic acid and a fairly high level of benzyl amino purine. The genomic constitution of the cultivar on one hand as well as the concentration of benzyl amino purine in the medium on the other hand seem to control the rate of multiplication of shoot cuds. Eventually, the multiplication rate was greatly accelerated by increasing the concentration of benzyl amino purine in the medium. However, the presence of one or two ‘B’ genomes which came from Musa balbisiana causes an increase in the rate of multiplication as compared to the cultivars possessing only ‘A’ genome, from Musa acuminata. Regarding the in-vitro conservation of banana germplasm, established shoot tip cultures could be successfully stored for over two years in the same container without any transfer to fresh medium by simply reducing the incubation temperature and light intensity from 30° to 15°C and 1000 lux respectively. The survival percentage of various cultivars at different stages of conservation have been critically analysed. The regeneration of somatic embryos through the induction of callus and their further studies have been briefly described in the last phase of the paper. The somatic embryos, when carefully analysed histologically, exhibited striking similarities with their zygotic counterparts.
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Claims 6
Claim · model-written summary
Banana explants multiplied rapidly on the supplemented basal medium.
- Taxon
- bananae0
- Treatment
- Murashige and Skoog's semi-solid basal medium supplemented with low concentration of indole acetic acid and a fairly high level of benzyl amino purinee0
- Outcome
- Rapid in-vitro multiplicatione0
Not reportedComparatorValue
Verbatim
e0“Rapid in-vitro multiplication of banana has been achieved by culturing the explants in Murashige and Skoog's semi-solid basal medium supplemented with low concentration of indole acetic acid and a fairly high level of benzyl amino purine”
Claim · model-written summary
Cultivar genomic constitution and benzyl amino purine concentration appeared to control shoot multiplication rate.
- Taxon
- Bananat0
- Material
- cultivare0
- Outcome
- rate of multiplication of shoot cudse0
- Qualifier
- concentration of benzyl amino purinee0
Not reportedDirection
Verbatim
e0“The genomic constitution of the cultivar on one hand as well as the concentration of benzyl amino purine in the medium on the other hand seem to control the rate of multiplication of shoot cuds”
t0“Banana”
Claim · model-written summary
Increasing benzyl amino purine concentration greatly accelerated shoot multiplication.
- Taxon
- Bananat0
- Treatment
- increasing the concentration of benzyl amino purinee0
- Outcome
- multiplication ratee0
- Direction
- acceleratede0
Not reportedComparator
Verbatim
e0“the multiplication rate was greatly accelerated by increasing the concentration of benzyl amino purine in the medium”
t0“Banana”
Claim · model-written summary
Cultivars with one or two B genomes multiplied faster than cultivars with only an A genome.
Verbatim
e0“the presence of one or two ‘B’ genomes which came from Musa balbisiana causes an increase in the rate of multiplication as compared to the cultivars possessing only ‘A’ genome, from Musa acuminata”
t0“Banana”
Claim · model-written summary
Banana shoot tip cultures were stored for over two years without transfer after reducing temperature and light intensity.
Verbatim
e0“established shoot tip cultures could be successfully stored for over two years in the same container without any transfer to fresh medium by simply reducing the incubation temperature and light intensity from 30° to 15°C and 1000 lux respectively”
t0“Banana”
Claim · model-written summary
Banana somatic embryos showed striking histological similarities to zygotic embryos.
Verbatim
e0“The somatic embryos, when carefully analysed histologically, exhibited striking similarities with their zygotic counterparts”
t0“Banana”
The same questions in other papers
aminopurine · pest · MusaResults agree
1 paper in the same direction as this paper
- positive
benzyl group · pest · MusaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.