Abstract
Twelve commercial sugarcane (Saccharum spp. hybrids) varieties were compared for both their ability to form white, embryogenic callus and their transformation potential. Of the 12 varieties investigated, NCo310, NCo376, N8, N12, N16, N18 and N20 produced large proportions of white, embryogenic (Type 3) callus in relation to total callus volume, especially when subcultured weekly on 1 mg l− 1 2,4-dichlorophenoxyacetic acid (2,4-D) for up to six weeks. Fortnightly subculture on 1 mg l− 1 2,4-D and on variable levels of 2,4-D were less effective in Type 3 callus maintenance. Transformation potential of the white callus from seven varieties was assessed by bombarding callus with a plasmid containing the genes which encode two anthocyanin transcriptional activators (pDP687). Expression of the inserted genes was measured after 48 h. Of all the varieties, NCo310 produced higher proportions of white callus, showed the best transient anthocyanin gene expression, and subsequent plantlet regeneration occurred, indicating that this variety is suitable for further transformation studies.
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Claims 7
Claim · model-written summary
Seven sugarcane varieties produced large proportions of white, embryogenic (Type 3) callus.
Verbatim
e0“Twelve commercial sugarcane (Saccharum spp. hybrids) varieties were compared for both their ability to form white, embryogenic callus and their transformation potential”
e1“NCo310, NCo376, N8, N12, N16, N18 and N20 produced large proportions of white, embryogenic (Type 3) callus in relation to total callus volume”
Claim · model-written summary
Fortnightly subculture on 1 mg/l 2,4-D was less effective than weekly subculture for Type 3 callus maintenance.
Verbatim
e0“Twelve commercial sugarcane (Saccharum spp. hybrids) varieties were compared for both their ability to form white, embryogenic callus and their transformation potential”
e2“Fortnightly subculture on 1 mg l− 1 2,4-D and on variable levels of 2,4-D were less effective in Type 3 callus maintenance”
e3“subcultured weekly on 1 mg l− 1 2,4-dichlorophenoxyacetic acid”
Claim · model-written summary
Variable levels of 2,4-D were less effective than weekly subculture on 1 mg/l 2,4-D for Type 3 callus maintenance.
Verbatim
e0“Twelve commercial sugarcane (Saccharum spp. hybrids) varieties were compared for both their ability to form white, embryogenic callus and their transformation potential”
e2“Fortnightly subculture on 1 mg l− 1 2,4-D and on variable levels of 2,4-D were less effective in Type 3 callus maintenance”
e3“subcultured weekly on 1 mg l− 1 2,4-dichlorophenoxyacetic acid”
Claim · model-written summary
NCo310 produced higher proportions of white callus than all the varieties tested.
Verbatim
e0“Twelve commercial sugarcane (Saccharum spp. hybrids) varieties were compared for both their ability to form white, embryogenic callus and their transformation potential”
e4“Of all the varieties, NCo310 produced higher proportions of white callus”
Claim · model-written summary
NCo310 showed the best transient anthocyanin gene expression of the varieties tested.
Verbatim
e0“Twelve commercial sugarcane (Saccharum spp. hybrids) varieties were compared for both their ability to form white, embryogenic callus and their transformation potential”
e4“Of all the varieties, NCo310 produced higher proportions of white callus”
e5“showed the best transient anthocyanin gene expression”
e6“a plasmid containing the genes which encode two anthocyanin transcriptional activators”
Claim · model-written summary
Plantlet regeneration occurred from NCo310 callus.
Verbatim
e0“Twelve commercial sugarcane (Saccharum spp. hybrids) varieties were compared for both their ability to form white, embryogenic callus and their transformation potential”
e4“Of all the varieties, NCo310 produced higher proportions of white callus”
e6“subsequent plantlet regeneration occurred”
Claim · model-written summary
NCo310 is suitable for further transformation studies.
Verbatim
e0“Twelve commercial sugarcane (Saccharum spp. hybrids) varieties were compared for both their ability to form white, embryogenic callus and their transformation potential”
e4“Of all the varieties, NCo310 produced higher proportions of white callus”
e7“suitable for further transformation studies”
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