Abstract
Shoot tips from in vitro stock plants of red chicory ‘Rosso di Chioggia’ line were cryopreserved by one-step vitrification. After two days of cold-hardening on hormone-free MS medium and loading for 30 min in a mixture of 2 M glycerol and 0.4 M sucrose at 25°C, shoot tips were dehydrated with PVS2 vitrification solution at 0°C for 60 min and plunged directly into liquid nitrogen. The post-thaw survival of shoot tips was achieved 79% when was cultured on recovery medium containing 0.5 mM BA. Observed regrowth, after six weeks of culture in the same medium composition, was 100%. Rooted cryopreserved microshoots showed good quality when transferred to the greenhouse. Preliminary results proved that the genetic fidelity of the cryopreserved line was maintained. The same vitrification protocol was then applied to three other red chicory lines, ‘Rosso di Treviso precoce’, ‘Rosso di Treviso tardivo’ and ‘Castelfranco’. A simple and effective protocol for the cryopreservation of red chicory shoot tips has been successfully developed as a result of this study.
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Claims 5
Claim · model-written summary
Post-thaw shoot tip survival reached 79% after vitrification and recovery on BA medium.
Verbatim
e0“The post-thaw survival of shoot tips was achieved 79% when was cultured on recovery medium containing 0.5 mM BA”
t0“red chicory”
e1“‘Rosso di Chioggia’ line”
Claim · model-written summary
Regrowth reached 100% after six weeks on the same recovery medium.
Verbatim
e0“Observed regrowth, after six weeks of culture in the same medium composition, was 100%”
t0“red chicory”
Claim · model-written summary
Rooted cryopreserved microshoots showed good quality after greenhouse transfer.
Verbatim
e0“Rooted cryopreserved microshoots showed good quality when transferred to the greenhouse”
t0“red chicory”
Claim · model-written summary
Preliminary results found maintained genetic fidelity in the cryopreserved line.
- Taxon
- red chicoryt0
- Outcome
- genetic fidelity of the cryopreserved linee0
- Direction
- maintainede0
- Comparator
- cryopreserved linee0
Not reportedValue
Verbatim
e0“Preliminary results proved that the genetic fidelity of the cryopreserved line was maintained”
t0“red chicory”
Claim · model-written summary
The vitrification protocol was successfully developed for cryopreservation of red chicory shoot tips.
Verbatim
e0“A simple and effective protocol for the cryopreservation of red chicory shoot tips has been successfully developed as a result of this study”