Abstract
Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world. Development of new repeatable and reliable tissue culture protocols is very important for improvement of breeding and genetic transformation studies. It is a highly recalcitrant plant and there is an urgent need to develop a regeneration protocol that can ensure easy multiple and qualitative superior shoots that could be rooted and yield fertile plants. Unconditioned plumular apices and plumular apices preconditioned with 10 mg L^{-1} benzylaminopurine (BA) for 10 days were cultured on Murashige and Skoog (MS) medium supplemented with 0.25-2.00 mg L^{-1} BA with or without 0.25 mg L^{-1} naphthalene acetic acid (NAA). Comparing the 2 culture conditions, the preconditioned explants had 2-fold to 5-fold more regeneration and shoots per explant compared to the unconditioned plumular apices. The presence of NAA in the culture medium positively increased the number of shoots per preconditioned explant at the lower concentrations of BA. In contrast, NAA inhibited the number of shoots per explants and the mean shoot length of the unconditioned explants at all concentrations of BA. An increased sucrose concentration of 45 and 60 g L^{-1} (R1) with 1.00 mg L^{-1} indole-3 butyric acid resulted in 50% rooting with multiple and earlier hardening of shoots. Subculturing of multiple shoots on MS medium containing 45-60 g L^{-1} sucrose (R2) enhanced the rooting frequency by 60%-100%. The rooted plantlets were successfully acclimatized.
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Claims 7
Claim · model-written summary
Preconditioned explants had twofold to fivefold more regeneration and shoots.
- Taxon
- Chickpea
Cicer arietinume1 - Treatment
- preconditioned explantse0
- Outcome
- regeneration and shoots per explante0
- Direction
- moree0
- Value
- 2-fold to 5-folde0
- Comparator
- unconditioned plumular apicese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the preconditioned explants had 2-fold to 5-fold more regeneration and shoots per explant compared to the unconditioned plumular apices”
e1“Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world.”
Claim · model-written summary
NAA increased shoots per preconditioned explant at lower BA concentrations.
- Taxon
- ChickpeaCicer arietinume1
- Treatment
- NAA in the culture mediume0
- Outcome
- number of shoots per preconditioned explante0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of NAA in the culture medium positively increased the number of shoots per preconditioned explant at the lower concentrations of BA”
e1“Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world.”
Claim · model-written summary
NAA inhibited shoot number in unconditioned explants.
- Taxon
- ChickpeaCicer arietinume1
- Treatment
- NAAe0
- Outcome
- number of shoots per explantse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAA inhibited the number of shoots per explants and the mean shoot length of the unconditioned explants at all concentrations of BA”
e1“Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world.”
Claim · model-written summary
NAA inhibited shoot length in unconditioned explants.
- Taxon
- ChickpeaCicer arietinume1
- Treatment
- NAAe0
- Outcome
- mean shoot lengthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NAA inhibited the number of shoots per explants and the mean shoot length of the unconditioned explants at all concentrations of BA”
e1“Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world.”
Claim · model-written summary
R1 medium gave 50% rooting and earlier hardening.
- Taxon
- ChickpeaCicer arietinume1
- Treatment
- increased sucrose concentration of 45 and 60 g L^{-1} (R1) with 1.00 mg L^{-1} indole-3 butyric acide0
- Outcome
- rootinge0
- Direction
- increasede0
- Value
- 50%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An increased sucrose concentration of 45 and 60 g L^{-1} (R1) with 1.00 mg L^{-1} indole-3 butyric acid resulted in 50% rooting with multiple and earlier hardening of shoots”
e1“Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world.”
Claim · model-written summary
R2 medium enhanced rooting frequency by 60%-100%.
- Taxon
- ChickpeaCicer arietinume1
- Treatment
- MS medium containing 45-60 g L^{-1} sucrose (R2)e0
- Outcome
- rooting frequencye0
- Direction
- enhancede0
- Value
- 60%-100%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subculturing of multiple shoots on MS medium containing 45-60 g L^{-1} sucrose (R2) enhanced the rooting frequency by 60%-100%”
e1“Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world.”
Claim · model-written summary
Rooted plantlets were acclimatized successfully.
- Taxon
- ChickpeaCicer arietinume1
- Outcome
- rooted plantlets were successfully acclimatizede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rooted plantlets were successfully acclimatized”
e1“Chickpea (Cicer arietinum L.) is an important grain legume used almost all over the world.”
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.