Abstract
The present study assesses the effects of 5-aminolevulinic acid (ALA, 0, 0.1, 1 and 10 mg l−1) on the growth of oilseed rape (Brassica napus L. cv. ZS758) seedlings under water-deficit stress induced by polyethylene glycol (PEG 6000, 0 and −0.3 MPa). Water-deficit stress imposed negative effects on seedling growth by reducing shoot biomass, cotyledon water potential, chlorophyll content and non-enzymatic antioxidants (glutathione and ascorbic acid) levels. On the other hand, water-deficit stress enhanced the malondialdehyde (MDA) content, reactive oxygen species (ROS) production, enzymatic antioxidants activities, reduced/oxidized glutathione ratio (GSH/GSSG) and reduced/oxidized ascorbic acid (ASA/DHA) ratio in seedlings. Application of ALA at lower dosages (0.1 and 1 mg l−1) improved shoot weight and chlorophyll contents, and decreased MDA in rape seedlings, whereas moderately higher dosage of ALA (10 mg l−1) hampered the growth. The study also indicated that 1 mg l−1 ALA improved chlorophyll content, but reduced MDA content and ROS production significantly under water-deficit stress. Lower dosages of ALA (0.1 and 1 mg l−1) also enhanced GSH/GSSG and ASA/DHA as compared to the seedlings under water-deficit stress. The antioxidant enzymes (ascorbate peroxidase, peroxidase, catalase, glutathione reductase and superoxide dismutase) enhanced their activities remarkably with 1 mg l−1 ALA treatment under water-deficit stress. It was also revealed that 1 mg l−1 ALA treatment alone induced the expression of APX, CAT and GR substantially and under water-deficit stress conditions ALA treatment could induce the expression of POD, CAT and GR to a certain degree. These results indicated that 0.1–1 mg l−1 ALA could enhance the water-deficit stress tolerance of oilseed seedlings through improving the biomass accumulation, maintaining a relative high ratio of GSH/GSSG and ASA/DHA, enhancing the activities of the specific antioxidant enzymes and inducing the expression of the specific antioxidant enzyme genes.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 13
Claim · model-written summary
Low-dose ALA improved shoot weight and chlorophyll contents of rape seedlings
- Taxon
- Brassica napus L.
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Material
- cv. ZS758e0
- Treatment
- Application of ALA at lower dosages (0.1 and 1 mg l−1)e1
- Outcome
- shoot weight and chlorophyll contentse1
- Direction
- improvede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e1“Application of ALA at lower dosages (0.1 and 1 mg l−1) improved shoot weight and chlorophyll contents, and decreased MDA in rape seedlings”
Claim · model-written summary
Low-dose ALA decreased MDA content in rape seedlings
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- Application of ALA at lower dosages (0.1 and 1 mg l−1)e1
- Outcome
- MDAe1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e1“Application of ALA at lower dosages (0.1 and 1 mg l−1) improved shoot weight and chlorophyll contents, and decreased MDA in rape seedlings”
Claim · model-written summary
A moderately higher ALA dose of 10 mg/l hampered seedling growth
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- moderately higher dosage of ALA (10 mg l−1)e2
- Outcome
- growthe2
- Direction
- hamperede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e2“moderately higher dosage of ALA (10 mg l−1) hampered the growth”
Claim · model-written summary
Water-deficit stress reduced shoot biomass, cotyledon water potential and chlorophyll content of seedlings
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- water-deficit stresse3
- Outcome
- shoot biomass, cotyledon water potential, chlorophyll contente3
- Direction
- reducinge3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e3“Water-deficit stress imposed negative effects on seedling growth by reducing shoot biomass, cotyledon water potential, chlorophyll content and non-enzymatic antioxidants (glutathione and ascorbic acid) levels”
Claim · model-written summary
Water-deficit stress reduced non-enzymatic antioxidant (glutathione and ascorbic acid) levels in seedlings
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- water-deficit stresse3
- Outcome
- non-enzymatic antioxidants (glutathione and ascorbic acid) levelse3
- Direction
- reducinge3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e3“Water-deficit stress imposed negative effects on seedling growth by reducing shoot biomass, cotyledon water potential, chlorophyll content and non-enzymatic antioxidants (glutathione and ascorbic acid) levels”
Claim · model-written summary
Water-deficit stress increased MDA content and reactive oxygen species production in seedlings
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- water-deficit stresse4
- Outcome
- malondialdehyde (MDA) content, reactive oxygen species (ROS) productione4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e4“water-deficit stress enhanced the malondialdehyde (MDA) content, reactive oxygen species (ROS) production”
Claim · model-written summary
The 1 mg/l ALA treatment improved chlorophyll content under water-deficit stress
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- 1 mg l−1 ALAe5
- Outcome
- chlorophyll contente5
- Direction
- improvede5
- Qualifier
- under water-deficit stresse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e5“1 mg l−1 ALA improved chlorophyll content, but reduced MDA content and ROS production significantly under water-deficit stress”
Claim · model-written summary
The 1 mg/l ALA treatment reduced MDA content and ROS production significantly under water-deficit stress
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- 1 mg l−1 ALAe5
- Outcome
- MDA content and ROS productione5
- Direction
- reducede5
- Qualifier
- under water-deficit stresse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e5“1 mg l−1 ALA improved chlorophyll content, but reduced MDA content and ROS production significantly under water-deficit stress”
Claim · model-written summary
Lower ALA doses enhanced GSH/GSSG and ASA/DHA ratios under water-deficit stress
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- Lower dosages of ALA (0.1 and 1 mg l−1)e6
- Outcome
- GSH/GSSG and ASA/DHAe6
- Direction
- enhancede6
- Comparator
- the seedlings under water-deficit stresse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e6“Lower dosages of ALA (0.1 and 1 mg l−1) also enhanced GSH/GSSG and ASA/DHA as compared to the seedlings under water-deficit stress”
Claim · model-written summary
The 1 mg/l ALA treatment enhanced activities of ascorbate peroxidase, peroxidase and catalase under water-deficit stress
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- 1 mg l−1 ALA treatmente7
- Outcome
- ascorbate peroxidase, peroxidase, catalasee7
- Direction
- enhancede7
- Qualifier
- under water-deficit stresse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e7“The antioxidant enzymes (ascorbate peroxidase, peroxidase, catalase, glutathione reductase and superoxide dismutase) enhanced their activities remarkably with 1 mg l−1 ALA treatment under water-deficit stress”
Claim · model-written summary
The 1 mg/l ALA treatment enhanced activities of glutathione reductase and superoxide dismutase under water-deficit stress
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- 1 mg l−1 ALA treatmente7
- Outcome
- glutathione reductase and superoxide dismutasee7
- Direction
- enhancede7
- Qualifier
- under water-deficit stresse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e7“The antioxidant enzymes (ascorbate peroxidase, peroxidase, catalase, glutathione reductase and superoxide dismutase) enhanced their activities remarkably with 1 mg l−1 ALA treatment under water-deficit stress”
Claim · model-written summary
The 1 mg/l ALA treatment alone substantially induced expression of APX, CAT and GR
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- 1 mg l−1 ALA treatment alonee1
- Gene
- APX, CAT and GRe1
- Outcome
- expressione1
- Direction
- inducede1
- Qualifier
- substantiallye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e1“1 mg l−1 ALA treatment alone induced the expression of APX, CAT and GR substantially”
Claim · model-written summary
Under water-deficit stress ALA could induce expression of POD, CAT and GR to a certain degree
- Taxon
- Brassica napus L.Brassica napuse0
- Treatment
- ALA treatmente1
- Gene
- POD, CAT and GRe1
- Outcome
- expressione1
- Direction
- inducee1
- Qualifier
- to a certain degreee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oilseed rape (Brassica napus L. cv. ZS758) seedlings”
e1“under water-deficit stress conditions ALA treatment could induce the expression of POD, CAT and GR to a certain degree”
The same questions in other papers
5-aminolevulinic acid · molecular: expression · Brassica napus (oilseed rape)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
5-aminolevulinic acid · physiology: oxidative · Brassica napus (oilseed rape)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- positive
5-aminolevulinic acid · growth · Brassica napus (oilseed rape)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
5-aminolevulinic acid · physiology: antioxidant enzymes · Brassica napus (oilseed rape)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
5-aminolevulinic acid · molecular: metabolites · Brassica napus (oilseed rape)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →drought · quality: phytochemicals · Brassica napus (oilseed rape)Positive and negative results
5 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
3 papers more in this group
See all 7 papers →drought · physiology: oxidative · Brassica napus (oilseed rape)Positive and negative results
21 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
23 papers more in this group
See all 27 papers →drought · physiology: water · Brassica napus (oilseed rape)Positive and negative results
25 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
27 papers more in this group
See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.