Abstract
AIMS: This work was conducted to identify the antifungal compounds produced by two previously isolated Bacillus sp. strains: ARP(2) 3 and MEP(2) 18. Both strains were subjected to further analysis to determine their taxonomic position and to identify the compounds responsible for their antifungal activity as well as to evaluate the efficiency of these strains to control sclerotinia stem rot in soybean. METHODS AND RESULTS: The antifungal compounds were isolated by acid precipitation of cell-free supernatants, purified by RP-HPLC and then tested for antagonistic activity against Sclerotinia sclerotiorum. Mass spectra from RP-HPLC eluted fractions showed the presence of surfactin C(15) , fengycins A (C(16) -C(17)) and B (C(16)) isoforms in supernatants from strain ARP(2) 3 cultures, whereas the major lipopeptide produced by strain MEP(2) 18 was iturin A C(15) . Alterations in mycelial morphology and sclerotial germination were observed in the presence of lipopeptides-containing supernatants from Bacillus strains cultures. Foliar application of Bacillus amyloliquefaciens strains on soybean plants prior to S. sclerotiorum infection resulted in significant protection against sclerotinia stem rot compared with noninoculated plants or plants inoculated with a nonlipopeptide-producing B. subtilis strain. CONCLUSIONS: Both strains, renamed as B. amyloliquefaciens ARP(2) 3 and MEP(2) 18, were able to produce antifungal compounds belonging to the cyclic lipopeptide family. Our data suggest that the foliar application of lipopeptide-producing B. amyloliquefaciens strains could be a promising strategy for the management of sclerotinia stem rot in soybean. SIGNIFICANCE AND IMPACT OF THE STUDY: Sclerotinia stem rot was ranked as one of the most severe soybean disease in Argentina and worldwide. The results of this study showed the potential of B. amyloliquefaciens strains ARP(2) 3 and MEP(2) 18 to control plant diseases caused by S. sclerotiorum.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
ARP(2) 3 cultures contained surfactin and fengycin isoforms.
- Taxon
- soybean
Glycine maxe1 - Interactor
- strain ARP(2) 3e0biocontrol_agent
- Outcome
- surfactin C(15) , fengycins A (C(16) -C(17)) and B (C(16)) isoformse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mass spectra from RP-HPLC eluted fractions showed the presence of surfactin C(15) , fengycins A (C(16) -C(17)) and B (C(16)) isoforms in supernatants from strain ARP(2) 3 cultures, whereas the major lipopeptide produced by strain MEP(2) 18 was iturin A C(15) .”
e1“to control sclerotinia stem rot in soybean.”
Claim · model-written summary
The major lipopeptide from MEP(2) 18 was iturin A C(15).
- Taxon
- soybeanGlycine maxe1
- Interactor
- strain MEP(2) 18e0biocontrol_agent
- Outcome
- iturin A C(15)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mass spectra from RP-HPLC eluted fractions showed the presence of surfactin C(15) , fengycins A (C(16) -C(17)) and B (C(16)) isoforms in supernatants from strain ARP(2) 3 cultures, whereas the major lipopeptide produced by strain MEP(2) 18 was iturin A C(15) .”
e1“to control sclerotinia stem rot in soybean.”
Claim · model-written summary
Bacillus lipopeptide-containing supernatants altered Sclerotinia mycelial morphology and sclerotial germination.
- Taxon
- soybeanGlycine maxe1
- Treatment
- lipopeptides-containing supernatantse0
- Interactor
- Bacillus strainse0biocontrol_agent
- Outcome
- mycelial morphology and sclerotial germinatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Alterations in mycelial morphology and sclerotial germination were observed in the presence of lipopeptides-containing supernatants from Bacillus strains cultures.”
e1“to control sclerotinia stem rot in soybean.”
Claim · model-written summary
Foliar Bacillus amyloliquefaciens significantly protected soybean against sclerotinia stem rot relative to both controls.
- Taxon
- soybeanGlycine maxe0
- Treatment
- Foliar application of Bacillus amyloliquefaciens strainse0
- Interactor
- S. sclerotiorum
Sclerotinia sclerotiorume0pathogen - Second interactor
- Bacillus amyloliquefaciens
.jpg?width=60)
Bacillus amyloliquefaciense0biocontrol_agent - Outcome
- protection against sclerotinia stem rote0
- Direction
- protectione0
- Comparator
- noninoculated plants or plants inoculated with a nonlipopeptide-producing B. subtilis straine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Foliar application of Bacillus amyloliquefaciens strains on soybean plants prior to S. sclerotiorum infection resulted in significant protection against sclerotinia stem rot compared with noninoculated plants or plants inoculated with a nonlipopeptide-producing B. subtilis strain.”
Claim · model-written summary
The authors suggest foliar lipopeptide-producing B. amyloliquefaciens for managing soybean sclerotinia stem rot.
- Taxon
- soybeanGlycine maxe0
- Interactor
- B. amyloliquefaciensBacillus amyloliquefaciense0biocontrol_agent
- Outcome
- foliar application of lipopeptide-producing B. amyloliquefaciens strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our data suggest that the foliar application of lipopeptide-producing B. amyloliquefaciens strains could be a promising strategy for the management of sclerotinia stem rot in soybean.”
The same questions in other papers
application · Sclerotinia sclerotiorum · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.