Abstract
The aim of this study was to compare the efficiency of broccoli anther and microspore culture methods for doubled haploid (DH) lines production. We evaluated the main influencing factors and optimized the culture methods to improve embryo induction and plant regeneration for efficient doubled haploid production in broccoli breeding. Six broccoli hybrids were used in this study. Our results show that generally, the efficiency of androgenic embryogenesis and regeneration in microspore culture is higher than that in the anther culture. Moreover, the microspore culture eliminated the possibility of plantlets coming from diploid tissue. In this study, the four-day cold pre-treatment yielded the highest number of embryos in both anther and microspore culture methods; the embryo yield at 32.5°C for 24 h was the highest in anther and microspore culture. Optimal plating densities were 30 anthers per dish in anther culture and 4×105 microspores per ml in microspore culture. In androgenic embryo production, the PG-96 medium proved to be more effective than NLN medium. Sucrose concentration at 10% for anther culture and 13% (w/v) for microspore culture was recommended. A total of 70 regenerants were obtained from three genotypes including doubled haploids, haploids and aneuploids. Key words: Anther, broccoli, doubled haploid, microspore, plant regeneration.
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Claims 10
Claim · model-written summary
Microspore culture generally yielded more efficient androgenic embryogenesis and regeneration than anther culture.
- Taxon
- broccoli
Brassica oleracea var. italicat0 - Treatment
- microspore culturee0
- Outcome
- efficiency of androgenic embryogenesis and regeneratione0
- Direction
- highere0
- Comparator
- anther culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the efficiency of androgenic embryogenesis and regeneration in microspore culture is higher than that in the anther culture”
t0“broccoli”
Claim · model-written summary
Microspore culture eliminated the possibility of plantlets originating from diploid tissue.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Outcome
- possibility of plantlets coming from diploid tissuee0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the microspore culture eliminated the possibility of plantlets coming from diploid tissue”
t0“broccoli”
Claim · model-written summary
Four-day cold pretreatment gave the highest embryo count in both culture methods.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- four-day cold pre-treatmente0
- Outcome
- number of embryose0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the four-day cold pre-treatment yielded the highest number of embryos in both anther and microspore culture methods”
t0“broccoli”
Claim · model-written summary
A 32.5°C treatment for 24 h gave the highest embryo yield in anther and microspore culture.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- 32.5°C for 24 he0
- Outcome
- embryo yielde0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the embryo yield at 32.5°C for 24 h was the highest in anther and microspore culture”
t0“broccoli”
Claim · model-written summary
The optimal anther plating density was 30 anthers per dish.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- anther culturee0
- Outcome
- Optimal plating densitiese0
- Value
- 30 anthers per dishe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal plating densities were 30 anthers per dish in anther culture and 4×105 microspores per ml in microspore culture”
t0“broccoli”
Claim · model-written summary
The optimal microspore plating density was 4×105 microspores per ml.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- microspore culturee0
- Outcome
- Optimal plating densitiese0
- Value
- 4×105 microspores per mle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal plating densities were 30 anthers per dish in anther culture and 4×105 microspores per ml in microspore culture”
t0“broccoli”
Claim · model-written summary
PG-96 medium was more effective than NLN medium for androgenic embryo production.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- PG-96 mediume0
- Outcome
- androgenic embryo productione0
- Direction
- more effectivee0
- Comparator
- NLN mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In androgenic embryo production, the PG-96 medium proved to be more effective than NLN medium”
t0“broccoli”
Claim · model-written summary
The authors recommended 10% sucrose for anther culture.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- Sucrosee0
- Outcome
- 10% for anther culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose concentration at 10% for anther culture and 13% (w/v) for microspore culture was recommended”
t0“broccoli”
Claim · model-written summary
The authors recommended 13% (w/v) sucrose for microspore culture.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Treatment
- Sucrosee0
- Outcome
- 13% (w/v) for microspore culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sucrose concentration at 10% for anther culture and 13% (w/v) for microspore culture was recommended”
t0“broccoli”
Claim · model-written summary
Seventy regenerants comprising doubled haploids, haploids and aneuploids were obtained from three genotypes.
- Taxon
- broccoliBrassica oleracea var. italicat0
- Outcome
- regenerantse0
- Value
- 70 regenerantse0
- Qualifier
- three genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 70 regenerants were obtained from three genotypes including doubled haploids, haploids and aneuploids”
t0“broccoli”
The same questions in other papers
in vitro · yield · Brassica oleracea (wild cabbage)Positive and negative results
10 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.