Abstract
1. In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents. 2. Chloroplast and cytoplasmic ribosomes were obtained in a ratio of 1 : 28 after selective isolation. 3. After freezing identical portions of cytoplasmic extract the amount of ribosomes/ml extract dropped, but later increased with freezing time. 4. Ribosomal preparations showed UV-absorption spectra typical for RNA. Extinction ratios suggested that preparations were contaminated with extraneous protein to a certain extent, purity increasing after selective isolation, especially after freezing. 5. Protein patterns and sedimentation behaviour of cytoplasmic ribosomes were identical regardless of addition or omission of detergent in leaf exeracts. 6. After polyacrylamide gel electrophoresis of ribosomal proteins, 29 bands of basic protein were found in cytoplasmic ribosomes and 22 in chloroplast ribosomes. Acidic proteins could not be detected. Patterns in both extracts were different and highly reproducible, resembling those recently reported. Tests for five enzymes, highly active in the leaf tissue, gave negative results on the isolated ribosomal proteins.
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Claims 13
Claim · model-written summary
An extraction medium with HEPES buffer, low Mg2+ and SH-group protecting agents gave the highest yield of total ribosomes from Phaseolus leaves.
- Organ / stage
- leavese0
- Treatment
- extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agentse0
- Outcome
- yielde0
- Direction
- higheste0
PhaseolusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
Claim · model-written summary
Chloroplast and cytoplasmic ribosomes were obtained in a ratio of 1 : 28 after selective isolation from Phaseolus leaves.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e1“Chloroplast and cytoplasmic ribosomes were obtained in a ratio of 1 : 28 after selective isolation”
Claim · model-written summary
Freezing of cytoplasmic extract decreased the amount of ribosomes per ml extract.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e2“After freezing identical portions of cytoplasmic extract the amount of ribosomes/ml extract dropped, but later increased with freezing time”
Claim · model-written summary
The amount of ribosomes per ml extract later increased with freezing time.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e2“After freezing identical portions of cytoplasmic extract the amount of ribosomes/ml extract dropped, but later increased with freezing time”
Claim · model-written summary
Ribosomal preparations showed UV-absorption spectra typical for RNA.
- Outcome
- UV-absorption spectrae3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e3“Ribosomal preparations showed UV-absorption spectra typical for RNA”
Claim · model-written summary
Selective isolation increased the purity of the ribosomal preparations.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e4“Extinction ratios suggested that preparations were contaminated with extraneous protein to a certain extent, purity increasing after selective isolation, especially after freezing”
Claim · model-written summary
Ribosomal preparations were contaminated with extraneous protein to a certain extent.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e4“Extinction ratios suggested that preparations were contaminated with extraneous protein to a certain extent, purity increasing after selective isolation, especially after freezing”
Claim · model-written summary
Protein patterns and sedimentation behaviour of cytoplasmic ribosomes were identical with or without detergent in Phaseolus leaf extracts.
- Treatment
- addition or omission of detergente5
- Outcome
- Protein patterns and sedimentation behaviour of cytoplasmic ribosomese5
- Comparator
- omission of detergente5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e5“Protein patterns and sedimentation behaviour of cytoplasmic ribosomes were identical regardless of addition or omission of detergent in leaf exeracts”
Claim · model-written summary
Cytoplasmic ribosomes gave 29 bands of basic protein after polyacrylamide gel electrophoresis.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e6“After polyacrylamide gel electrophoresis of ribosomal proteins, 29 bands of basic protein were found in cytoplasmic ribosomes and 22 in chloroplast ribosomes”
Claim · model-written summary
Chloroplast ribosomes gave 22 bands of basic protein after polyacrylamide gel electrophoresis.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e6“After polyacrylamide gel electrophoresis of ribosomal proteins, 29 bands of basic protein were found in cytoplasmic ribosomes and 22 in chloroplast ribosomes”
Claim · model-written summary
Acidic proteins could not be detected in ribosomal preparations from Phaseolus leaves.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e7“Acidic proteins could not be detected”
Claim · model-written summary
Protein patterns differed between cytoplasmic and chloroplast ribosomes and were highly reproducible.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e7“Patterns in both extracts were different and highly reproducible, resembling those recently reported”
Claim · model-written summary
Tests for five highly active leaf enzymes were negative on the isolated ribosomal proteins.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e7“Tests for five enzymes, highly active in the leaf tissue, gave negative results on the isolated ribosomal proteins”