Claim · model-written summary
Selective isolation increased the purity of the ribosomal preparations.
PhaseolusNot reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleComparatorSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In isolation of total ribosomes from Phaseolus leaves the highest yield was obtained with an extraction medium containing HEPES buffer, low Mg2+-concentration (1 mM) and SH-group protecting agents”
e4“Extinction ratios suggested that preparations were contaminated with extraneous protein to a certain extent, purity increasing after selective isolation, especially after freezing”
Title
Extraction and comparison of bean cytoplasmic and chloroplast ribosomes
CHRISTIAN ARGLEBE; TIMOTHY C. HALL Plant and Cell Physiology 1969. doi:10.1093/oxfordjournals.pcp.a074382
Other claims from this paper (12)
- Intervention effect An extraction medium with HEPES buffer, low Mg2+ and SH-group protecting agents gave the highest yield of total ribosomes from Phaseolus leaves.
- Observation Chloroplast and cytoplasmic ribosomes were obtained in a ratio of 1 : 28 after selective isolation from Phaseolus leaves.
- Intervention effect Freezing of cytoplasmic extract decreased the amount of ribosomes per ml extract.
- Intervention effect The amount of ribosomes per ml extract later increased with freezing time.
- Observation Ribosomal preparations showed UV-absorption spectra typical for RNA.
- Observation Ribosomal preparations were contaminated with extraneous protein to a certain extent.
- Intervention effect Protein patterns and sedimentation behaviour of cytoplasmic ribosomes were identical with or without detergent in Phaseolus leaf extracts.
- Observation Cytoplasmic ribosomes gave 29 bands of basic protein after polyacrylamide gel electrophoresis.
- Observation Chloroplast ribosomes gave 22 bands of basic protein after polyacrylamide gel electrophoresis.
- Observation Acidic proteins could not be detected in ribosomal preparations from Phaseolus leaves.
- Observation Protein patterns differed between cytoplasmic and chloroplast ribosomes and were highly reproducible.
- Observation Tests for five highly active leaf enzymes were negative on the isolated ribosomal proteins.