Abstract
The expression of host genes can be altered during the process of viral infection. To investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with Sugarcane mosaic virus (SCMV), a suppression subtractive hybridization cDNA library was constructed. A total of 454 cDNA clones were identified to be viral infection-induced up-regulated genes. The influence of Rop1 on the infection of maize by SCMV was investigated. The results showed that transient silencing of the ZmRop1 gene through virus-induced gene silencing enhanced the accumulation and systemic infection of SCMV and another potyvirus (Pennisetum mosaic virus) in maize plants, whereas transient over-expression of ZmRop1 in maize protoplasts reduced SCMV accumulation. Furthermore, it was demonstrated that the heterologous expression of ZmRop1 impaired Potato virus X infection in Nicotiana benthamiana plants. These data suggest that ZmRop1 may play a role in plant defence responses to viral infection.
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Claims 5
Claim · model-written summary
Viral infection induced up-regulation in 454 maize cDNA clones.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 454 cDNA clones were identified to be viral infection-induced up-regulated genes.”
t0“maize”
Claim · model-written summary
Transient ZmRop1 silencing enhanced SCMV accumulation and systemic infection in maize.
- Treatment
- transient silencing of the ZmRop1 genee0
- Interactor
- SCMV
Sugarcane mosaic viruse0pathogen - Gene
- ZmRop1e0
- Outcome
- accumulation and systemic infection of SCMVe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient silencing of the ZmRop1 gene through virus-induced gene silencing enhanced the accumulation and systemic infection of SCMV and another potyvirus (Pennisetum mosaic virus) in maize plants”
Claim · model-written summary
Transient ZmRop1 silencing enhanced Pennisetum mosaic virus accumulation and systemic infection in maize.
- Treatment
- transient silencing of the ZmRop1 genee0
- Interactor
- Pennisetum mosaic virusPennisetum mosaic viruse0pathogen
- Gene
- ZmRop1e0
- Outcome
- accumulation and systemic infectione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient silencing of the ZmRop1 gene through virus-induced gene silencing enhanced the accumulation and systemic infection of SCMV and another potyvirus (Pennisetum mosaic virus) in maize plants”
Claim · model-written summary
Transient ZmRop1 over-expression reduced SCMV accumulation in maize protoplasts.
- Organ / stage
- protoplastse0
- Treatment
- transient over-expression of ZmRop1e0
- Interactor
- SCMVSugarcane mosaic viruse0pathogen
- Gene
- ZmRop1e0
- Outcome
- SCMV accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient over-expression of ZmRop1 in maize protoplasts reduced SCMV accumulation”
Claim · model-written summary
Heterologous ZmRop1 expression impaired Potato virus X infection in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- heterologous expression of ZmRop1e0
- Interactor
- Potato virus XPotato virus Xe0pathogen
- Gene
- ZmRop1e0
- Outcome
- Potato virus X infectione0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heterologous expression of ZmRop1 impaired Potato virus X infection in Nicotiana benthamiana plants”
The same questions in other papers
overexpression · Potato virus X · Nicotiana benthamianaPositive and negative results
4 in the opposite direction
- negative
- negative
- negative
1 paper more in this group
See all 5 papers →loss of function · Sugarcane mosaic virus · Zea mays (maize)Positive and negative results
5 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.