Abstract
An efficient protocol for in vitro micropropagation of V. encelioides (Cav.) Benth. & Hook. fil. ex Gray, a medicinal weed, through hypocotyl segments of seedlings was developed on Murashige and Skoog’s (MS) medium supplemented with various concentrations of α-naphthalene acetic acid (NAA, 0.50-15.00 mg/L), 6-benzylaminopurine (BAP, 1.00-3.00 mg/L) and kinetin (Kn, 0.20-0.60 mg/L). Highly organized callus induction and proliferation were achieved on MS medium+NAA (1.00 mg/L)+BAP (3.00 mg/L). Optimum shoot multiplication (13.00±0.28) was achieved on MS medium+NAA (0.50 mg/L)+BAP (1.00 mg/L) with the shoot height of 1.80± 0.15 cm. Similarly, efficient root induction (8.30±0.40) was achieved on MS medium+indole 3-acetic acid (IAA, 1.00 mg/L) with the root length of 2.60± 0.34 cm as 83% of the microshoots produced roots. These plantlets were hardened in a controlled plant growth chamber, prior to ex-vitro transfer. No phenotypic differences were observed among the regenerates.
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Claims 7
Claim · model-written summary
The specified NAA and BAP medium induced organized callus proliferation.
- Treatment
- MS medium+NAA (1.00 mg/L)+BAP (3.00 mg/L)e0
- Outcome
- Highly organized callus induction and proliferatione0
- Value
- 1.00 mg/Le0
Not reportedTaxon
Verbatim
e0“Highly organized callus induction and proliferation were achieved on MS medium+NAA (1.00 mg/L)+BAP (3.00 mg/L).”
Claim · model-written summary
The specified medium produced optimum shoot multiplication of 13.00±0.28.
Not reportedTaxon
Verbatim
e0“Optimum shoot multiplication (13.00±0.28) was achieved on MS medium+NAA (0.50 mg/L)+BAP (1.00 mg/L) with the shoot height of 1.80± 0.15 cm.”
Claim · model-written summary
Shoots reached 1.80± 0.15 cm on the optimum shoot medium.
Not reportedTaxon
Verbatim
e0“with the shoot height of 1.80± 0.15 cm.”
Claim · model-written summary
The specified IAA medium induced 8.30±0.40 roots.
Not reportedTaxon
Verbatim
e0“efficient root induction (8.30±0.40) was achieved on MS medium+indole 3-acetic acid (IAA, 1.00 mg/L) with the root length of 2.60± 0.34 cm as 83% of the microshoots produced roots.”
Claim · model-written summary
Roots reached 2.60± 0.34 cm.
Not reportedTaxon
Verbatim
e0“with the root length of 2.60± 0.34 cm”
Claim · model-written summary
Eighty-three percent of microshoots rooted.
Not reportedTaxon
Verbatim
e0“83% of the microshoots produced roots.”
Claim · model-written summary
Regenerates showed no phenotypic differences.
Not reportedTaxon
Verbatim
e0“No phenotypic differences were observed among the regenerates.”