Abstract
S ummary A novel method is described in which the two halves of an explanted internode of Lycopersicon esculentum, Datura stramonium or Nicandra physaloides may be grafted together successfully in sterile culture. An absolute requirement for the formation of a successful graft is the application of indole‐3‐acetic acid (IAA at 0.2 to 2.0 mg l −1 ) to the apical end of the internode. The addition of kinetin (0.2 mg l −1 ) to the culture medium stimulated graft development but gibberellic acid (GA 3 at 0 − 5 mg l −1 ) was inhibitory. The development of the graft as measured by an increase in mechanical strength and the formation of vascular connections was similar to that observed in whole plant grafts; however, fewer differentiated wound vessel members (WVMs) were detected. This technique should provide a powerful tool for the further study of graft development in compatible and incompatible combinations.
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Claims 8
Claim · model-written summary
Explanted internode halves of Lycopersicon esculentum could be grafted in sterile culture.
- Taxon
- Lycopersicon esculentum
Solanum lycopersicume0 - Outcome
- grafted together successfullye0
- Comparator
- sterile culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the two halves of an explanted internode of Lycopersicon esculentum, Datura stramonium or Nicandra physaloides may be grafted together successfully in sterile culture”
Claim · model-written summary
Explanted internode halves of Datura stramonium could be grafted in sterile culture.
- Taxon
- Datura stramonium
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Datura stramoniume0 - Outcome
- grafted together successfullye0
- Comparator
- sterile culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“the two halves of an explanted internode of Lycopersicon esculentum, Datura stramonium or Nicandra physaloides may be grafted together successfully in sterile culture”
Claim · model-written summary
Explanted internode halves of Nicandra physaloides could be grafted in sterile culture.
- Taxon
- Nicandra physaloides
Nicandra physalodese0 - Outcome
- grafted together successfullye0
- Comparator
- sterile culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the two halves of an explanted internode of Lycopersicon esculentum, Datura stramonium or Nicandra physaloides may be grafted together successfully in sterile culture”
Claim · model-written summary
IAA application to the apical internode end was required for successful graft formation.
- Taxon
- Lycopersicon esculentum, Datura stramonium or Nicandra physaloidesDatura stramoniumSolanum lycopersicumNicandra physalodese1
- Organ / stage
- internodee0
- Treatment
- indole‐3‐acetic acid (IAA at 0.2 to 2.0 mg l −1 )e0
- Outcome
- formation of a successful grafte0
- Direction
- absolute requiremente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An absolute requirement for the formation of a successful graft is the application of indole‐3‐acetic acid (IAA at 0.2 to 2.0 mg l −1 ) to the apical end of the internode”
e1“Lycopersicon esculentum, Datura stramonium or Nicandra physaloides”
Claim · model-written summary
Kinetin in culture medium stimulated graft development.
- Taxon
- Lycopersicon esculentum, Datura stramonium or Nicandra physaloidesDatura stramoniumSolanum lycopersicumNicandra physalodese1
- Treatment
- kinetin (0.2 mg l −1 )e0
- Outcome
- graft developmente0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of kinetin (0.2 mg l −1 ) to the culture medium stimulated graft development”
e1“Lycopersicon esculentum, Datura stramonium or Nicandra physaloides”
Claim · model-written summary
Gibberellic acid inhibited graft development.
- Taxon
- Lycopersicon esculentum, Datura stramonium or Nicandra physaloidesDatura stramoniumSolanum lycopersicumNicandra physalodest0
- Treatment
- gibberellic acid (GA 3 at 0 − 5 mg l −1 )e0
- Outcome
- graft developmente1
- Direction
- inhibitorye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gibberellic acid (GA 3 at 0 − 5 mg l −1 ) was inhibitory”
e1“graft development but gibberellic acid (GA 3 at 0 − 5 mg l −1 ) was inhibitory”
t0“Lycopersicon esculentum, Datura stramonium or Nicandra physaloides”
Claim · model-written summary
Cultured grafts developed mechanical strength and vascular connections similarly to whole plant grafts.
- Taxon
- Lycopersicon esculentum, Datura stramonium or Nicandra physaloidesDatura stramoniumSolanum lycopersicumNicandra physalodese1
- Outcome
- increase in mechanical strength and the formation of vascular connectionse0
- Direction
- similare0
- Comparator
- whole plant graftse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The development of the graft as measured by an increase in mechanical strength and the formation of vascular connections was similar to that observed in whole plant grafts”
e1“Lycopersicon esculentum, Datura stramonium or Nicandra physaloides”
Claim · model-written summary
Cultured grafts formed fewer differentiated wound vessel members than whole plant grafts.
- Taxon
- Lycopersicon esculentum, Datura stramonium or Nicandra physaloidesDatura stramoniumSolanum lycopersicumNicandra physalodese2
- Outcome
- differentiated wound vessel members (WVMs)e0
- Direction
- fewere0
- Comparator
- whole plant graftse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fewer differentiated wound vessel members (WVMs) were detected”
e1“whole plant grafts; however, fewer differentiated wound vessel members (WVMs) were detected”
e2“Lycopersicon esculentum, Datura stramonium or Nicandra physaloides”
The same questions in other papers
gibberellin A3 · growth · Solanum lycopersicum (tomato)Positive and negative results
2 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
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4 papers more in this group
See all 8 papers →kinetin · growth · Solanum lycopersicum (tomato)Positive and negative results
21 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
20 papers more in this group
See all 24 papers →kinetin · growth · Datura stramonium (jimsonweed)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.