Paper · 2012 · Chemistry & Biodiversity

Application of UV/VIS Spectrophotometry and Multivariate Analysis to Characterization of the Species of Solanum sect. ErythrotrichumChild

Ionaldo José L. D. Basílio; Renata K. P. Moura; Jnanabrata Bhattacharyya; et al.

7
claims
10
verbatim quotes
3
citations
2
research areas
—
Not open access

Abstract

The UV/VIS spectral characteristics of the standardized extracts of the leaves of 22 Solanum species of the Leptostemonum clade were investigated in the presence of shift reagents with the aid of multivariate analysis, to obtain data in support of the interspecific and subsectional delimitation proposed for Solanum sect. Erythrotrichum. Of these species, 20 belong to the section Erythrotrichum, S. paniculatum is assigned to the section Torva, and S. robustum is not attributed to a defined section. The results indicated characteristic λ(max) (absorbance maxima) for each species as well as the presence of phenolic compounds like flavonoids such as 5-hydroxy flavonols. Hierarchical cluster analysis (HCA) of the data obtained by UV/VIS analysis of the extracts or the extracts with the added shift reagents AlCl₃ and HCl showed a cophenetic correlation coefficient above 0.92 and the classification of the data into three groups. The principal-component analysis (PCA) revealed that the first three principal components accounted for over 98% of the total variance and showed results similar to those obtained by HCA. The present results supported the current proposal for interspecific delimitation of the studied species and partially supported the division of the section into two subsections. The UV/VIS spectral characteristics along with multivariate analysis appear to be a useful approach for distinguishing among species of the genus Solanum.

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Claims 7

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Each Solanum species showed characteristic UV/VIS absorbance maxima, consistent with flavonoids such as 5-hydroxy flavonols.

Taxon
SolanumSolanume0
Outcome
characteristic λ(max) (absorbance maxima) for each speciese1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum
Solanum · Taxon · picture of the genus (one of its species)Forest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the leaves of 22 Solanum species of the Leptostemonum clade were investigated”

✓ located verbatim in the source · abstract, characters 68–145 · show in text ↑
e1

“The results indicated characteristic λ(max) (absorbance maxima) for each species as well as the presence of phenolic compounds like flavonoids such as 5-hydroxy flavonols”

✓ located verbatim in the source · abstract, characters 503–673 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Hierarchical cluster analysis of UV/VIS data gave a cophenetic correlation coefficient above 0.92 and three groups.

Outcome
cophenetic correlation coefficiente2
Value
0.92e2

Verbatim

e2

“showed a cophenetic correlation coefficient above 0.92 and the classification of the data into three groups”

✓ located verbatim in the source · abstract, characters 827–934 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The first three principal components explained over 98% of the total variance of the UV/VIS data.

Outcome
total variancee3
Value
98%e3

Verbatim

e3

“The principal-component analysis (PCA) revealed that the first three principal components accounted for over 98% of the total variance”

✓ located verbatim in the source · abstract, characters 936–1070 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Principal component analysis gave results similar to those from hierarchical cluster analysis.

Outcome
resultse4
Comparator
those obtained by HCAe4

Verbatim

e4

“showed results similar to those obtained by HCA”

✓ located verbatim in the source · abstract, characters 1075–1122 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The UV/VIS results supported the current proposal for interspecific delimitation of Solanum species.

Taxon
SolanumSolanume0
Outcome
current proposal for interspecific delimitatione5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum
Solanum · Taxon · picture of the genus (one of its species)Forest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the leaves of 22 Solanum species of the Leptostemonum clade were investigated”

✓ located verbatim in the source · abstract, characters 68–145 · show in text ↑
e5

“The present results supported the current proposal for interspecific delimitation of the studied species”

✓ located verbatim in the source · abstract, characters 1124–1228 · show in text ↑
Observationobservedoriginal resultmixedmeasured

Claim · model-written summary

The UV/VIS results only partly supported dividing the section into two subsections.

Taxon
SolanumSolanume0
Outcome
division of the section into two subsectionse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum
Solanum · Taxon · picture of the genus (one of its species)Forest & Kim Starr · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the leaves of 22 Solanum species of the Leptostemonum clade were investigated”

✓ located verbatim in the source · abstract, characters 68–145 · show in text ↑
e6

“partially supported the division of the section into two subsections”

✓ located verbatim in the source · abstract, characters 1233–1301 · show in text ↑
Method / resourceauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

UV/VIS spectra with multivariate analysis appear useful for distinguishing Solanum species.

Outcome
distinguishing among speciese7

Not reportedComparator

Verbatim

e7

“The UV/VIS spectral characteristics along with multivariate analysis appear to be a useful approach for distinguishing among species of the genus Solanum”

✓ located verbatim in the source · abstract, characters 1303–1456 · show in text ↑