Abstract
Jatropha curcas is a species with a variety of uses. It is grown primarily for oil for biodiesel, but also has agronomic and medicinal applications. Two methods were evaluated for cryopreservation of seeds and zygotic embryos of J. curcas: desiccation followed by rapid immersion of seeds and embryos in liquid nitrogen (LN, -196°C), and vitrification of zygotic embryos. Prior to cryo-preservation, seeds were manually scarified and the moisture content (MC) of seeds and embryos was determined. Explants were disinfected after cryopreservation. Seed germination after LN exposure was 100%. Plantlet development was better in sand substrate than that in vitro. Survival of zygotic embryos after cryopreservation was also 100%, without significant differences between treatments. Optimal development (100%) and plantlet length (51.77 mm) were observed with embryos dried for 60 min to 9.4% MC under laminar flow prior to cryopreservation. Zygotic embryos subjected to the vitrification procedure did not withstand LN exposure. Survival data for non-cryopreserved embryos after each step of the vitrification procedure provided information about embryo tolerance to cryoprotectants.
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Claims 7
Claim · model-written summary
Seed germination after liquid nitrogen exposure was 100%.
- Taxon
- Jatropha curcas L.
_tree_at_Samedha.jpg?width=60)
Jatropha curcast0 - Treatment
- LN exposuree0
- Outcome
- Seed germinatione0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_tree_at_Samedha.jpg?width=500)
Verbatim
e0“Seed germination after LN exposure was 100%.”
t0“Jatropha curcas L.”
Claim · model-written summary
Plantlet development was better in sand substrate than in vitro.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- sand substratee0
- Outcome
- Plantlet developmente0
- Direction
- bettere0
- Comparator
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plantlet development was better in sand substrate than that in vitro.”
t0“Jatropha curcas L.”
Claim · model-written summary
Zygotic embryo survival after cryopreservation was 100%, without significant treatment differences.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- cryopreservatione0
- Outcome
- Survival of zygotic embryose0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Survival of zygotic embryos after cryopreservation was also 100%, without significant differences between treatments.”
t0“Jatropha curcas L.”
Claim · model-written summary
Embryos dried for 60 min to 9.4% moisture before cryopreservation had optimal development of 100%.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- embryos dried for 60 min to 9.4% MC under laminar flow prior to cryopreservatione0
- Outcome
- Optimal developmente0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal development (100%) and plantlet length (51.77 mm) were observed with embryos dried for 60 min to 9.4% MC under laminar flow prior to cryopreservation.”
t0“Jatropha curcas L.”
Claim · model-written summary
Embryos dried for 60 min to 9.4% moisture before cryopreservation had plantlet length of 51.77 mm.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- embryos dried for 60 min to 9.4% MC under laminar flow prior to cryopreservatione0
- Outcome
- plantlet lengthe0
- Value
- 51.77 mme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal development (100%) and plantlet length (51.77 mm) were observed with embryos dried for 60 min to 9.4% MC under laminar flow prior to cryopreservation.”
t0“Jatropha curcas L.”
Claim · model-written summary
Vitrified zygotic embryos did not withstand liquid nitrogen exposure.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Treatment
- vitrification proceduree0
- Outcome
- did not withstand LN exposuree0
- Comparator
- vitrification proceduree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Zygotic embryos subjected to the vitrification procedure did not withstand LN exposure.”
t0“Jatropha curcas L.”
Claim · model-written summary
Survival of non-cryopreserved embryos after vitrification steps provided information about cryoprotectant tolerance.
- Taxon
- Jatropha curcas L.Jatropha curcast0
- Outcome
- embryo tolerance to cryoprotectantse0
- Comparator
- non-cryopreserved embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Survival data for non-cryopreserved embryos after each step of the vitrification procedure provided information about embryo tolerance to cryoprotectants.”
t0“Jatropha curcas L.”
The same questions in other papers
growing medium · growth · Jatropha curcasPositive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.