Abstract
A simplified, highly reproducible procedure is outlined for the extraction and polyacrylamide gel disc electrophoresis of acidic soluble proteins from apple bark and arborvitae foliage. The procedure includes low temperature maceration, short extraction time, and an extraction solution which contains polyol and phenolic complexers, a reducing agent, and a non-ionic surfactant. Electrophoretic patterns, total nitrogen, moisture content, minimum survival temperatures, and environmental and morphological changes were examined during the natural dehardening of apple and the controlled hardening of arborvitae. Qualitative protein changes, as evidenced by the appearance and disappearance of specific bands, occurred at times when changes in hardiness were taking place.
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Claims 4
Claim · model-written summary
A reproducible extraction and electrophoresis procedure was outlined for apple bark proteins.
- Taxon
- apple
Malus domesticae0 - Outcome
- highly reproducible proceduree0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A simplified, highly reproducible procedure is outlined for the extraction and polyacrylamide gel disc electrophoresis of acidic soluble proteins from apple bark and arborvitae foliage”
Claim · model-written summary
A reproducible extraction and electrophoresis procedure was outlined for arborvitae foliage proteins.
Not reportedComparatorValue
Verbatim
e0“A simplified, highly reproducible procedure is outlined for the extraction and polyacrylamide gel disc electrophoresis of acidic soluble proteins from apple bark and arborvitae foliage”
Claim · model-written summary
Protein bands appeared and disappeared as hardiness changed in apple.
- Taxon
- appleMalus domesticae1
- Organ / stage
- barke2
- Outcome
- Qualitative protein changese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Qualitative protein changes, as evidenced by the appearance and disappearance of specific bands, occurred at times when changes in hardiness were taking place”
e1“natural dehardening of apple”
e2“A simplified, highly reproducible procedure is outlined for the extraction and polyacrylamide gel disc electrophoresis of acidic soluble proteins from apple bark and arborvitae foliage”
Claim · model-written summary
Protein bands appeared and disappeared as hardiness changed in arborvitae.
Not reportedDirection
Verbatim
e0“Qualitative protein changes, as evidenced by the appearance and disappearance of specific bands, occurred at times when changes in hardiness were taking place”
e1“controlled hardening of arborvitae”
e2“A simplified, highly reproducible procedure is outlined for the extraction and polyacrylamide gel disc electrophoresis of acidic soluble proteins from apple bark and arborvitae foliage”