Abstract
Abstract: Specific inhibitors of the secretory pathway represent important tools for investigation of cell wall synthesis and tip growth in pollen tubes. Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM. Ultrastructural investigation of pollen tube cytoplasm showed that brefeldin A caused the appearance of reticular structures and “brefeldin A compartments” containing unesterified pectins. Monensin caused inhibition of pollen tube germination at 80 nM. The drug induced swelling of the Golgi cisternae, many of which contained methyl‐esterified pectins. Cytochalasin D was effective at 1 μg/ml, the inhibition of germination being fully reversible. Application of the drug caused accumulation of secretory vesicles containing methyl‐esterified pectin around the dictyosomes. In contrast to brefeldin A and monensin, cytochalasin D caused a slowdown of cytoplasmic streaming. Monensin, but not the other drugs, caused a considerable decrease in pollen tube diameter. The characterization and quantification of the effects of the drugs on pollen tubes represents a necessary prerequisite for their application in physiological studies.
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Claims 8
Claim · model-written summary
Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0 - Treatment
- Brefeldin Ae0
- Outcome
- germinatione0
- Direction
- completely inhibitse0
- Value
- 2.2 μMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM”
Claim · model-written summary
Brefeldin A caused the appearance of reticular structures in pollen tube cytoplasm.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- brefeldin Ae1
- Outcome
- reticular structurese1
- Direction
- caused the appearancee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM”
e1“brefeldin A caused the appearance of reticular structures”
Claim · model-written summary
Monensin inhibits pollen tube germination at 80 nM.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- Monensine1
- Outcome
- pollen tube germinatione1
- Direction
- inhibitione1
- Value
- 80 nMe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM”
e1“Monensin caused inhibition of pollen tube germination at 80 nM”
Claim · model-written summary
Monensin induced swelling of the Golgi cisternae.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- Monensine1
- Outcome
- swelling of the Golgi cisternaee2
- Direction
- inducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM”
e1“Monensin caused inhibition of pollen tube germination at 80 nM”
e2“The drug induced swelling of the Golgi cisternae”
Claim · model-written summary
Cytochalasin D inhibits pollen tube germination at 1 μg/ml, and the inhibition is fully reversible.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- Cytochalasin De1
- Outcome
- germinatione1
- Direction
- inhibitione1
- Value
- 1 μg/mle1
- Qualifier
- fully reversiblee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM”
e1“Cytochalasin D was effective at 1 μg/ml, the inhibition of germination being fully reversible”
Claim · model-written summary
Cytochalasin D caused accumulation of secretory vesicles around the dictyosomes.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- Cytochalasin De1
- Outcome
- secretory vesiclese2
- Direction
- accumulatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM”
e1“Cytochalasin D was effective at 1 μg/ml, the inhibition of germination being fully reversible”
e2“Application of the drug caused accumulation of secretory vesicles”
Claim · model-written summary
Cytochalasin D caused a slowdown of cytoplasmic streaming in pollen tubes.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- cytochalasin De1
- Outcome
- cytoplasmic streaminge1
- Direction
- slowdowne1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM”
e1“cytochalasin D caused a slowdown of cytoplasmic streaming”
Claim · model-written summary
Monensin caused a considerable decrease in pollen tube diameter.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- Monensine1
- Outcome
- pollen tube diametere1
- Direction
- considerable decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Brefeldin A completely inhibits germination of Nicotiana tabacum pollen tubes at 2.2 μM”
e1“Monensin, but not the other drugs, caused a considerable decrease in pollen tube diameter”