Abstract
In the present study, an efficient ex-vitro rooting technique was developed for sugarcane commercial cultivar CoS 96268 based on the assessment of several factors. Axillary bud cultures of nodal segments were used in this experiment. The parameters optimized for ex vitro rooting includes the effects of auxin concentration, propagule size, shoot size, duration of auxin pulse treatment, sucrose concentration in multiplication medium, ex-vitro rooting medium were studied. The results showed that the auxin concentration @ 20 mg/l gave maximum rooting percentage. Among 0h, 6h,12h and 24h treatment duration with auxin, 24h treatment duration developed better roots. 90 to 95% rooting was observed in three shoot propagule with 5 to 6 cm length of in vitro micro shoots. It has been observed that sucrose concentration in multiplication medium also influenced the ex vitro rooting. Sucrose at 45 g/l gave good results. Sand was best rooting medium among the peat, peat + soil and vermiculite for the development of ex vitro roots in green house conditions. Ex vitro rooted plants were transplanted in field and 9098% survival percent was found same as in vitro propagated plants of sugarcane.
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Claims 6
Claim · model-written summary
An auxin concentration of 20 mg/l gave the maximum rooting percentage.
- Taxon
- sugarcanee0
- Material
- CoS 96268e0
- Treatment
- auxin concentration @ 20 mg/le1
- Outcome
- rooting percentagee1
- Direction
- maximume1
Not reportedComparator
Verbatim
e0“an efficient ex-vitro rooting technique was developed for sugarcane commercial cultivar CoS 96268”
e1“the auxin concentration @ 20 mg/l gave maximum rooting percentage”
Claim · model-written summary
A 24 h auxin treatment duration developed better roots than 0, 6 or 12 h.
Verbatim
e0“an efficient ex-vitro rooting technique was developed for sugarcane commercial cultivar CoS 96268”
e1“Among 0h, 6h,12h and 24h treatment duration with auxin, 24h treatment duration developed better roots”
Claim · model-written summary
Rooting of 90 to 95% was observed in three-shoot propagules with 5 to 6 cm in vitro micro shoots.
Verbatim
e0“an efficient ex-vitro rooting technique was developed for sugarcane commercial cultivar CoS 96268”
e1“90 to 95% rooting was observed in three shoot propagule with 5 to 6 cm length of in vitro micro shoots”
Claim · model-written summary
Sucrose at 45 g/l in the multiplication medium gave good ex vitro rooting results.
- Taxon
- sugarcanee0
- Material
- CoS 96268e0
- Treatment
- Sucrose at 45 g/le1
- Outcome
- ex vitro rootinge2
- Direction
- good resultse1
- Value
- 45 g/le1
Not reportedComparator
Verbatim
e0“an efficient ex-vitro rooting technique was developed for sugarcane commercial cultivar CoS 96268”
e1“Sucrose at 45 g/l gave good results”
e2“sucrose concentration in multiplication medium also influenced the ex vitro rooting”
Claim · model-written summary
Sand was the best rooting medium for ex vitro root development in greenhouse conditions, better than peat, peat + soil and vermiculite.
Verbatim
e0“an efficient ex-vitro rooting technique was developed for sugarcane commercial cultivar CoS 96268”
e1“Sand was best rooting medium among the peat, peat + soil and vermiculite for the development of ex vitro roots in green house conditions”
Claim · model-written summary
Ex vitro rooted plants transplanted in the field had 9098% survival, the same as in vitro propagated plants.
Verbatim
e0“an efficient ex-vitro rooting technique was developed for sugarcane commercial cultivar CoS 96268”
e1“Ex vitro rooted plants were transplanted in field and 9098% survival percent was found same as in vitro propagated plants of sugarcane”
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