Abstract
The use of plant growth regulators is directly related to the success of in vitro propagation, which is an advantageous alternative to obtain seedlings on a commercial scale. This study aimed to evaluate the in vitro development of ‘IAC 95-5000’ sugarcane seedlings after the addition of different doses of ethephon (0, 25, 50, 100 and 200 mg L-1) or gibberellic acid (0, 2.5, 5.0, 7.5 and 10.0 mg L-1) to the culture medium. Ethephon increased the number of tillers (up to 231.70%), reduced height of the main tiller (44.66 to 60.47%), and did not affect the shoot´s fresh and dry mass. On the other hand, gibberellin decreased the number of tillers and negatively changed biomass partitioning. It is concluded that the use of ethephon is a potential strategy to enhance in vitro production of ‘IAC 95-5000’ sugarcane seedlings, since it increased the number of usable shoots in subsequent subcultures, and its effects on height reduction can be reversible. However, the use of the tested doses of gibberellic acid is not recommended, because it impaired seedling development of this sugarcane variety.
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Claims 8
Claim · model-written summary
Ethephon increased tiller number by up to 231.70%.
Not reportedComparator
Verbatim
e0“Ethephon increased the number of tillers (up to 231.70%)”
t0“sugarcane”
Claim · model-written summary
Ethephon reduced main tiller height by 44.66 to 60.47%.
- Taxon
- sugarcanet0
- Treatment
- Ethephone1
- Outcome
- height of the main tillere0
- Direction
- reducede0
- Value
- 44.66 to 60.47%e0
Not reportedComparator
Verbatim
e0“reduced height of the main tiller (44.66 to 60.47%)”
e1“Ethephon increased the number of tillers”
t0“sugarcane”
Claim · model-written summary
Ethephon did not affect shoot fresh or dry mass.
Not reportedComparator
Verbatim
e0“did not affect the shoot´s fresh and dry mass”
e1“Ethephon increased the number of tillers”
t0“sugarcane”
Claim · model-written summary
Gibberellin decreased tiller number.
Not reportedComparator
Verbatim
e0“gibberellin decreased the number of tillers”
t0“sugarcane”
Claim · model-written summary
Gibberellin negatively changed biomass partitioning.
Not reportedComparator
Verbatim
e0“gibberellin decreased the number of tillers and negatively changed biomass partitioning”
t0“sugarcane”
Claim · model-written summary
Ethephon increased usable shoots in subsequent subcultures.
- Taxon
- sugarcanet0
- Treatment
- ethephone1
- Outcome
- number of usable shootse0
- Direction
- increasede0
- Qualifier
- subsequent subculturese0
Not reportedComparator
Verbatim
e0“it increased the number of usable shoots in subsequent subcultures”
e1“the use of ethephon is a potential strategy”
t0“sugarcane”
Claim · model-written summary
The authors recommend ethephon for in vitro production of this sugarcane variety.
Verbatim
e0“the use of ethephon is a potential strategy to enhance in vitro production of ‘IAC 95-5000’ sugarcane seedlings”
Claim · model-written summary
The authors do not recommend the tested gibberellic acid doses for this sugarcane variety.
Verbatim
e0“the use of the tested doses of gibberellic acid is not recommended, because it impaired seedling development of this sugarcane variety”
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