Abstract
We examined the nitrogenase reductase (nifH) genes of endophytic diazotrophic bacteria expressed in field-grown sweet potatoes (Ipomoea batatas L.) by reverse transcription (RT)-PCR. Gene fragments corresponding to nifH were amplified from mRNA obtained from the stems and storage roots of field-grown sweet potatoes several months after planting. Sequence analysis revealed that these clones were homologous to the nifH sequences of Bradyrhizobium, Pelomonas, and Bacillus sp. in the DNA database. Investigation of the nifH genes amplified from the genomic DNA extracted from these sweet potatoes also showed high similarity to various α-proteobacteria including Bradyrhizobium, β-proteobacteria, and cyanobacteria. These results suggest that bradyrhizobia colonize and express nifH genes not only in the root nodules of leguminous plants but also in sweet potatoes as diazotrophic endophytes.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
nifH gene fragments were amplified from sweet potato stem and storage-root mRNA.
- Taxon
- sweet potatoes
_Flower.jpg?width=60)
Ipomoea batatase0 - Organ / stage
- stems and storage rootse0
- Gene
- nifHe0
- Outcome
- Gene fragments corresponding to nifH were amplifiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
e0“Gene fragments corresponding to nifH were amplified from mRNA obtained from the stems and storage roots of field-grown sweet potatoes several months after planting.”
Claim · model-written summary
Expressed clones were homologous to Bradyrhizobium nifH sequences.
- Taxon
- Sweet PotatoesIpomoea batatast0
- Interactor
- Bradyrhizobium
Bradyrhizobiume0symbiont - Gene
- nifHe0
- Outcome
- homologous to the nifH sequencese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potatoes”
e0“these clones were homologous to the nifH sequences of Bradyrhizobium, Pelomonas, and Bacillus sp. in the DNA database”
Claim · model-written summary
Expressed clones were homologous to Pelomonas nifH sequences.
- Taxon
- Sweet PotatoesIpomoea batatast0
- Interactor
- PelomonasRoseatelese0symbiont
- Gene
- nifHe0
- Outcome
- homologous to the nifH sequencese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potatoes”
e0“these clones were homologous to the nifH sequences of Bradyrhizobium, Pelomonas, and Bacillus sp. in the DNA database”
Claim · model-written summary
Expressed clones were homologous to Bacillus sp. nifH sequences.
- Taxon
- Sweet PotatoesIpomoea batatast0
- Interactor
- Bacillus sp.e0symbiont
- Gene
- nifHe0
- Outcome
- homologous to the nifH sequencese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Sweet Potatoes”
e0“these clones were homologous to the nifH sequences of Bradyrhizobium, Pelomonas, and Bacillus sp. in the DNA database”
Claim · model-written summary
Genomic nifH amplicons showed high similarity to diverse bacterial sequences.
- Taxon
- sweet potatoesIpomoea batatase0
- Gene
- nifHe0
- Outcome
- high similarity to various α-proteobacteria including Bradyrhizobium, β-proteobacteria, and cyanobacteriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nifH genes amplified from the genomic DNA extracted from these sweet potatoes also showed high similarity to various α-proteobacteria including Bradyrhizobium, β-proteobacteria, and cyanobacteria”
Claim · model-written summary
Bradyrhizobia may colonize sweet potatoes and express nifH as endophytes.
- Taxon
- sweet potatoesIpomoea batatase0
- Interactor
- bradyrhizobiae0symbiont
- Gene
- nifHe0
- Outcome
- colonize and express nifH genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bradyrhizobia colonize and express nifH genes not only in the root nodules of leguminous plants but also in sweet potatoes as diazotrophic endophytes”