Abstract
Using a whole-genome-sequencing approach to explore germplasm resources can serve as an important strategy for crop improvement, especially in investigating wild accessions that may contain useful genetic resources that have been lost during the domestication process. Here we sequence and assemble a draft genome of wild soybean and construct a recombinant inbred population for genotyping-by-sequencing and phenotypic analyses to identify multiple QTLs relevant to traits of interest in agriculture. We use a combination of de novo sequencing data from this work and our previous germplasm re-sequencing data to identify a novel ion transporter gene, GmCHX1, and relate its sequence alterations to salt tolerance. Rapid gain-of-function tests show the protective effects of GmCHX1 towards salt stress. This combination of whole-genome de novo sequencing, high-density-marker QTL mapping by re-sequencing and functional analyses can serve as an effective strategy to unveil novel genomic information in wild soybean to facilitate crop improvement. The identification of genes that control economically important traits is an essential step towards crop improvement. Here the authors sequence the genome of the wild soybean and, through a combined genetic and functional approach, identify a new gene affecting salt tolerance in soybean.
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Claims 6
Claim · model-written summary
A draft genome of wild soybean was sequenced and assembled.
- Taxon
- wild soybean
Glycine sojat0 - Outcome
- draft genomee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wild soybean”
e0“we sequence and assemble a draft genome of wild soybean”
Claim · model-written summary
A recombinant inbred population was constructed for genotyping-by-sequencing and phenotypic analyses to identify multiple QTLs relevant to agricultural traits.
- Taxon
- wild soybeanGlycine sojat0
- Outcome
- recombinant inbred populatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wild soybean”
e1“construct a recombinant inbred population for genotyping-by-sequencing and phenotypic analyses to identify multiple QTLs relevant to traits of interest in agriculture”
Claim · model-written summary
A novel ion transporter gene, GmCHX1, was identified in wild soybean.
- Taxon
- wild soybeanGlycine sojat0
- Gene
- GmCHX1e2
- Outcome
- novel ion transporter genee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wild soybean”
e2“identify a novel ion transporter gene, GmCHX1”
Claim · model-written summary
Sequence alterations of GmCHX1 were related to salt tolerance.
- Taxon
- wild soybeanGlycine sojat0
- Gene
- sequence alterationse3
- Outcome
- salt tolerancee3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wild soybean”
e3“relate its sequence alterations to salt tolerance”
Claim · model-written summary
Rapid gain-of-function tests showed protective effects of GmCHX1 towards salt stress.
- Taxon
- wild soybeanGlycine sojat0
- Treatment
- gain-of-functione4
- Gene
- GmCHX1e4
- Outcome
- protective effectse4
- Direction
- protectivee4
- Qualifier
- salt stresse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wild soybean”
e4“Rapid gain-of-function tests show the protective effects of GmCHX1 towards salt stress”
Claim · model-written summary
The combination of whole-genome de novo sequencing, high-density-marker QTL mapping by re-sequencing and functional analyses can serve as an effective strategy to unveil novel genomic information in wild soybean for crop improvement.
- Taxon
- wild soybeanGlycine sojae5
- Outcome
- effective strategy to unveil novel genomic informatione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“can serve as an effective strategy to unveil novel genomic information in wild soybean to facilitate crop improvement”