Abstract
ABSTRACT Plant genetic engineering has long been considered a valuable tool to fight fungal pathogens because it would limit the economically costly and environmentally undesirable chemical methods of disease control. Ribosome-inactivating proteins (RIPs) are potentially useful for plant defense considering their antiviral and antimicrobial activities but their use is limited by their cytotoxic activity. A new gene coding for an RIP isolated from leaves of Phytolacca heterotepala was expressed in tobacco under the control of the wound-inducible promoter of the bean polygalacturonase-inhibiting protein I gene to increase resistance against different fungal pathogens, because an individual RIP isolated from P. heterotepala showed direct antifungal toxicity. Phenotypically normal transgenic lines infected with Alternaria alternata and Botrytis cinerea showed a significant reduction of leaf damage while reverse transcription-polymerase chain reaction and western analysis indicated the expression of the RIP transgene upon wounding and pathogen attack. This work demonstrates that use of a wound-inducible promoter is useful to limit the accumulation of antimicrobial phytotoxic proteins only in infected areas and that the controlled expression of the PhRIP I gene can be very effective to control fungal pathogens with different phytopathogenic actions.
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Claims 4
Claim · model-written summary
Transgenic tobacco had less leaf damage after Alternaria alternata infection.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Treatment
- transgenic linese0
- Interactor
- Alternaria alternata
.jpg?width=60)
Alternaria alternatae0pathogen - Outcome
- leaf damagee0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Phenotypically normal transgenic lines infected with Alternaria alternata and Botrytis cinerea showed a significant reduction of leaf damage”
t0“Tobacco”
Claim · model-written summary
Transgenic tobacco had less leaf damage after Botrytis cinerea infection.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- transgenic linese0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Outcome
- leaf damagee0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phenotypically normal transgenic lines infected with Alternaria alternata and Botrytis cinerea showed a significant reduction of leaf damage”
t0“Tobacco”
Claim · model-written summary
Wounding and pathogen attack induced RIP transgene expression.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- wounding and pathogen attacke0
- Gene
- RIP transgenee0
- Outcome
- expression of the RIP transgenee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reverse transcription-polymerase chain reaction and western analysis indicated the expression of the RIP transgene upon wounding and pathogen attack”
t0“Tobacco”
Claim · model-written summary
The isolated RIP showed direct antifungal toxicity.
- Taxon
- P. heterotepalaPhytolacca heterotepalae0
- Gene
- RIPe0
- Outcome
- direct antifungal toxicitye0
Verbatim
e0“an individual RIP isolated from P. heterotepala showed direct antifungal toxicity”