By organism › Fungi › Sclerotiniaceae › Botrytis cinerea

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The assay distinguished L412F and L412V mutations using diagnostic bands.

Taxon: Botrytis cinereaBotrytis cinereat0
Outcome: detect the nucleotide alterationse0
Value: 702 bpe0

Verbatim

e0
“A TETRA-primer amplification refractory mutation system polymerase chain reaction (T-ARMS-PCR) was developed to rapidly detect the nucleotide alterations that lead to L412F and L412V mutations, producing a 702 bp band in all isolates, with additional 470 bp (F) or 252 bp (V) fragments”
✓ located after whitespace/quote normalisation · abstract, characters 510–795

t0
“Botrytis cinerea”
✓ located verbatim in the source · title, characters 14–30

Title

Resistance of Botrytis cinerea to anilinopyrimidine fungicides: A novel ARMS ‐ PCR method for the detection of Bcpos5 mutations and characterization of resistance using CRISPR /Cas9 editing

Georgios Sofianos; Athanasios Petmezas; Anastasios Samaras; et al. Pest Management Science 2026. doi:10.1002/ps.70864

Other claims from this paper (9)

Method / resource The assay detected G408V with MlyI digestion.
Method / resource Sanger sequencing validated the assay results.
Observation L412F occurred in 70.2% of resistant isolates from strawberry and tomato.
Observation L412V occurred in 8.3% of resistant isolates from strawberry and tomato.
Observation G408V occurred in 4.7% of resistant isolates from strawberry and tomato.
Observation Sequencing found a low frequency of E407K in Bcmdl1.
Observation Sequencing indicated additional undefined resistance mechanisms.
Intervention effect L412F and L412V edited mutants resisted cyprodinil.
Observation L412F and L412V transformants showed no significant fitness difference from the parental strain.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

The assay detected G408V with MlyI digestion.

Taxon: Botrytis cinereaBotrytis cinereat0
Outcome: confirm the mutatione0
Value: 467 bp + 235 bpe0

Verbatim

e0
“Isolates harboring only the 702 bp band were further digested with MlyI to confirm the mutation leading to the amino acid substitution G408V mutation (467 bp + 235 bp)”
✓ located verbatim in the source · abstract, characters 797–964

t0
“Botrytis cinerea”
✓ located verbatim in the source · title, characters 14–30

Title

Resistance of Botrytis cinerea to anilinopyrimidine fungicides: A novel ARMS ‐ PCR method for the detection of Bcpos5 mutations and characterization of resistance using CRISPR /Cas9 editing

Georgios Sofianos; Athanasios Petmezas; Anastasios Samaras; et al. Pest Management Science 2026. doi:10.1002/ps.70864

Other claims from this paper (9)

Method / resource The assay distinguished L412F and L412V mutations using diagnostic bands.
Method / resource Sanger sequencing validated the assay results.
Observation L412F occurred in 70.2% of resistant isolates from strawberry and tomato.
Observation L412V occurred in 8.3% of resistant isolates from strawberry and tomato.
Observation G408V occurred in 4.7% of resistant isolates from strawberry and tomato.
Observation Sequencing found a low frequency of E407K in Bcmdl1.
Observation Sequencing indicated additional undefined resistance mechanisms.
Intervention effect L412F and L412V edited mutants resisted cyprodinil.
Observation L412F and L412V transformants showed no significant fitness difference from the parental strain.

Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

Sanger sequencing validated the assay results.

Taxon: Botrytis cinereaBotrytis cinereat0
Outcome: Results were validatede0
Comparator: Sanger sequencinge0

Verbatim

e0
“Results were validated by Sanger sequencing”
✓ located verbatim in the source · abstract, characters 966–1009

t0
“Botrytis cinerea”
✓ located verbatim in the source · title, characters 14–30

Title

Resistance of Botrytis cinerea to anilinopyrimidine fungicides: A novel ARMS ‐ PCR method for the detection of Bcpos5 mutations and characterization of resistance using CRISPR /Cas9 editing

Georgios Sofianos; Athanasios Petmezas; Anastasios Samaras; et al. Pest Management Science 2026. doi:10.1002/ps.70864

Other claims from this paper (9)

Method / resource The assay distinguished L412F and L412V mutations using diagnostic bands.
Method / resource The assay detected G408V with MlyI digestion.
Observation L412F occurred in 70.2% of resistant isolates from strawberry and tomato.
Observation L412V occurred in 8.3% of resistant isolates from strawberry and tomato.
Observation G408V occurred in 4.7% of resistant isolates from strawberry and tomato.
Observation Sequencing found a low frequency of E407K in Bcmdl1.
Observation Sequencing indicated additional undefined resistance mechanisms.
Intervention effect L412F and L412V edited mutants resisted cyprodinil.
Observation L412F and L412V transformants showed no significant fitness difference from the parental strain.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Sequencing found a low frequency of E407K in Bcmdl1.

Taxon: Botrytis cinereaBotrytis cinereat0
Gene: Bcmdl1e0
Outcome: E407K mutatione0

Verbatim

e0
“sequencing analysis revealed also a low frequency of the E407K mutation in Bcmdl1”
✓ located verbatim in the source · abstract, characters 1248–1329

t0
“Botrytis cinerea”
✓ located verbatim in the source · title, characters 14–30

Title

Resistance of Botrytis cinerea to anilinopyrimidine fungicides: A novel ARMS ‐ PCR method for the detection of Bcpos5 mutations and characterization of resistance using CRISPR /Cas9 editing

Georgios Sofianos; Athanasios Petmezas; Anastasios Samaras; et al. Pest Management Science 2026. doi:10.1002/ps.70864

Other claims from this paper (9)

Method / resource The assay distinguished L412F and L412V mutations using diagnostic bands.
Method / resource The assay detected G408V with MlyI digestion.
Method / resource Sanger sequencing validated the assay results.
Observation L412F occurred in 70.2% of resistant isolates from strawberry and tomato.
Observation L412V occurred in 8.3% of resistant isolates from strawberry and tomato.
Observation G408V occurred in 4.7% of resistant isolates from strawberry and tomato.
Observation Sequencing indicated additional undefined resistance mechanisms.
Intervention effect L412F and L412V edited mutants resisted cyprodinil.
Observation L412F and L412V transformants showed no significant fitness difference from the parental strain.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Sequencing indicated additional undefined resistance mechanisms.

Taxon: Botrytis cinereaBotrytis cinereat0
Outcome: resistance mechanismse0
Qualifier: additional, yet undefinede0

Verbatim

e0
“evidence of additional, yet undefined, resistance mechanisms”
✓ located verbatim in the source · abstract, characters 1342–1402

t0
“Botrytis cinerea”
✓ located verbatim in the source · title, characters 14–30

Title

Resistance of Botrytis cinerea to anilinopyrimidine fungicides: A novel ARMS ‐ PCR method for the detection of Bcpos5 mutations and characterization of resistance using CRISPR /Cas9 editing

Georgios Sofianos; Athanasios Petmezas; Anastasios Samaras; et al. Pest Management Science 2026. doi:10.1002/ps.70864

Other claims from this paper (9)

Method / resource The assay distinguished L412F and L412V mutations using diagnostic bands.
Method / resource The assay detected G408V with MlyI digestion.
Method / resource Sanger sequencing validated the assay results.
Observation L412F occurred in 70.2% of resistant isolates from strawberry and tomato.
Observation L412V occurred in 8.3% of resistant isolates from strawberry and tomato.
Observation G408V occurred in 4.7% of resistant isolates from strawberry and tomato.
Observation Sequencing found a low frequency of E407K in Bcmdl1.
Intervention effect L412F and L412V edited mutants resisted cyprodinil.
Observation L412F and L412V transformants showed no significant fitness difference from the parental strain.

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

L412F and L412V edited mutants resisted cyprodinil.

Taxon: Botrytis cinereaBotrytis cinereat0
Treatment: cyprodinile0
Outcome: resistancee0

Not reportedComparatorDirection

Verbatim

e0
“The resulting mutants displayed resistance to cyprodinil”
✓ located verbatim in the source · abstract, characters 1573–1629

t0
“Botrytis cinerea”
✓ located verbatim in the source · title, characters 14–30

Title

Resistance of Botrytis cinerea to anilinopyrimidine fungicides: A novel ARMS ‐ PCR method for the detection of Bcpos5 mutations and characterization of resistance using CRISPR /Cas9 editing

Georgios Sofianos; Athanasios Petmezas; Anastasios Samaras; et al. Pest Management Science 2026. doi:10.1002/ps.70864

Other claims from this paper (9)

Method / resource The assay distinguished L412F and L412V mutations using diagnostic bands.
Method / resource The assay detected G408V with MlyI digestion.
Method / resource Sanger sequencing validated the assay results.
Observation L412F occurred in 70.2% of resistant isolates from strawberry and tomato.
Observation L412V occurred in 8.3% of resistant isolates from strawberry and tomato.
Observation G408V occurred in 4.7% of resistant isolates from strawberry and tomato.
Observation Sequencing found a low frequency of E407K in Bcmdl1.
Observation Sequencing indicated additional undefined resistance mechanisms.
Observation L412F and L412V transformants showed no significant fitness difference from the parental strain.

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The compounds showed antifungal activity against Botrytis cinerea.

Taxon: Botrytis cinereaBotrytis cinereae0
Outcome: broad-spectrum antifungal activitye0

Verbatim

e0
“Additionally, the compounds exhibited broad-spectrum antifungal activity against Magnaporthe oryzae and Botrytis cinerea, while no phytotoxic effects were observed on treated plant seedlings.”
✓ located verbatim in the source · abstract, characters 878–1069

Title

Targeting the transcription factor AbaA: a potential strategy for inhibiting fungal conidiation and enhancing crop safety

Yanxiao Bi; Yan Ma; Xiangmei Zhang; et al. Phytopathology Research 2026. doi:10.1186/s42483-026-00416-x

Other claims from this paper (6)

Intervention effect G305-0126 inhibited Fol conidiation with an EC50 of 1.827 μM.
Intervention effect 8019-6157 inhibited Fol conidiation with an EC50 of 0.8849 μM.
Intervention effect The two compounds did not affect basal fungal growth.
Observation The compounds directly bound FolAbaA and downregulated three downstream genes.
Observation The compounds showed antifungal activity against Magnaporthe oryzae.
Intervention effect The compounds caused no observed phytotoxic effects on treated plant seedlings.

Observationobservedoriginal resultmeasured

Claim · model-written summary

Five dominant fungal taxa accounted for more than 90% of rhizosphere mycobiota.

Taxon: Fusarium oxysporum, F. solani, Phoma betae, Rhizoctonia solani, and Botrytis cinereaFusarium oxysporumBotrytis cinereaFusarium solaniRhizoctonia solaniNeocamarosporium betaee0
Outcome: rhizosphere mycobiotae0
Direction: dominante0
Value: more than 90%e0

Verbatim

e0
“The dominant taxa included Fusarium oxysporum, F. solani, Phoma betae, Rhizoctonia solani, and Botrytis cinerea, accounting for more than 90% of the rhizosphere mycobiota”
✓ located verbatim in the source · abstract, characters 333–503

Title

Species composition and population structure of phytopathogenic fungi in sugar beet agrocenoses of shortrotation crop rotations in the western forest-steppe of Ukraine

Kyselov, D.; Kalenska, S. Open MIND 2026. doi:10.15159/ar.26.008

Other claims from this paper (17)

Observation The study identified 240 fungal isolates from root and rhizosphere samples.
Observation Fusarium was most abundant at 48 ± 3.5%, forming a stable infectious core.
Association Short rotations increased F. oxysporum isolation frequency by 25–30% compared with other systems.
Association Short rotations increased Phoma betae isolation frequency by 18–22% compared with other systems.
Observation Rhizoctonia solani share reached 16% in maize rotations.
Association Soil type significantly influenced fungal species profiles.
Observation F. oxysporum predominated at approximately 35% in calcareous sandy soils.
Observation F. solani was dominant in chernozems.
Observation P. betae was dominant in chernozems.
Observation Fusarium isolates varied in pigmentation and sporulation, suggesting adaptability and potential virulent races.
Observation Fungal alpha diversity was low, with H′ of 1.13–1.32.
Observation Fungal dominance was high, with D of 0.42–0.51.
Observation Fusarium spp. were classified as primary pathogens.
Observation Phoma betae was classified as an opportunist.
Observation Rhizoctonia was classified as a necrotroph.
Observation Botrytis was classified as a necrotroph.
Recommendation The authors recommend integrating biocontrol and soil sanitation to restore balance and reduce infection pressure.

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pichia kluyveri B(F8) markedly inhibited Botrytis mycelial growth.

Taxon: B. cinereaBotrytis cinereae0
Treatment: Pichia kluyveri B(F8)e0
Interactor: Pichia kluyveri B(F8)Pichia kluyverie0biocontrol_agent
Outcome: mycelial growthe1
Direction: inhibitede1

Not reportedComparator

Verbatim

e0
“Pichia kluyveri B(F8) and Pichia kudriavzevii C(A4), were assessed for antagonistic activity against B. cinerea strains B05.10 and 992”
✓ located verbatim in the source · abstract, characters 183–317

e1
“Both yeasts markedly inhibited mycelial growth and significantly reduced disease severity on fruit.”
✓ located verbatim in the source · abstract, characters 457–556

Title

Distinct physiological strategies underpin antagonism of native Pichia yeasts against Botrytis cinerea

María Hernández-Fernández; José Luis Padilla-Agudelo; Gustavo Cordero-Bueso; et al. Food Microbiology 2026. doi:10.1016/j.fm.2026.105112

Other claims from this paper (8)

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pichia kudriavzevii C(A4) markedly inhibited Botrytis mycelial growth.

Taxon: B. cinereaBotrytis cinereae0
Treatment: Pichia kudriavzevii C(A4)e0
Interactor: Pichia kudriavzevii C(A4)Pichia kudriavzeviie0biocontrol_agent
Outcome: mycelial growthe1
Direction: inhibitede1

Not reportedComparator

Verbatim

e0
“Pichia kluyveri B(F8) and Pichia kudriavzevii C(A4), were assessed for antagonistic activity against B. cinerea strains B05.10 and 992”
✓ located verbatim in the source · abstract, characters 183–317

e1
“Both yeasts markedly inhibited mycelial growth and significantly reduced disease severity on fruit.”
✓ located verbatim in the source · abstract, characters 457–556

Title

Distinct physiological strategies underpin antagonism of native Pichia yeasts against Botrytis cinerea

María Hernández-Fernández; José Luis Padilla-Agudelo; Gustavo Cordero-Bueso; et al. Food Microbiology 2026. doi:10.1016/j.fm.2026.105112

Other claims from this paper (8)

Botrytis cinerea 549 claims

549 claims