Abstract
Indian citrus ringspot virus (ICRSV)-free plants of Kinnow mandarin (Citrus nobilis Lourx C. deliciosa Tenora) were produced from a virus-infected plant using nucellar embryo culture. The parent kinnow plant was tested by indirect ELISA and RT-PCR before using its explants. An amplified product of 539 bp (partial cp gene) was obtained by RT-PCR in ICRSV infected plants. The nucellar embryos obtained from seeds collected from immature fruits of the infected plant were cultured on Murashige and Skoog's (MS) basal medium supplemented with various concentrations of 2,4-D and malt extract (ME) alone or in various combinations. Maximum embryogenic callus induction (33.33%) was observed on MS medium supplemented with 2,4-D (9.02 μM) in combination with malt extract (400 mg L-1). Transfer of embryogenic calli to MS medium containing different concentrations of malt extract alone or in combination with ABA resulted in somatic embryogenesis with a maximum of 56.94% cultures in MS medium supplemented with malt extract (500 mg L-1) and ABA (7.56 μM). Cotyledonary shaped embryos when transferred to different strengths of MS medium supplemented with NAA (10.74 μM) developed into complete plantlets in maximum of 72.22% cultures on ½ MS medium. The plantlets were successfully acclimatizated, transferred to screen house and indexed for ICRSV employing indirect ELISA and RT-PCR and all were found negative of virus. A distinct feature of this study is the induction of somatic embryogenesis from nucellar embryos to produce virus-free plants.
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Claims 6
Claim · model-written summary
Nucellar embryo culture produced virus-free Kinnow mandarin plants from an infected plant.
- Taxon
- Kinnow mandarine0
- Interactor
- Indian citrus ringspot virusIndian citrus ringspot viruse0pathogen
- Outcome
- ICRSV)-free plantse0
- Comparator
- virus-infected plante0
Verbatim
e0“Indian citrus ringspot virus (ICRSV)-free plants of Kinnow mandarin (Citrus nobilis Lourx C. deliciosa Tenora) were produced from a virus-infected plant using nucellar embryo culture”
Claim · model-written summary
RT-PCR amplified a 539 bp partial coat protein gene product in infected plants.
Verbatim
e0“An amplified product of 539 bp (partial cp gene) was obtained by RT-PCR in ICRSV infected plants”
t0“Kinnow Mandarin”
Claim · model-written summary
The specified 2,4-D and malt extract medium produced maximum embryogenic callus induction of 33.33%.
- Taxon
- Kinnow mandarint0
- Treatment
- MS medium supplemented with 2,4-D (9.02 μM) in combination with malt extract (400 mg L-1)e0
- Outcome
- embryogenic callus inductione0
- Direction
- Maximume0
- Value
- 33.33%e0
Not reportedComparator
Verbatim
e0“Maximum embryogenic callus induction (33.33%) was observed on MS medium supplemented with 2,4-D (9.02 μM) in combination with malt extract (400 mg L-1)”
t0“Kinnow Mandarin”
Claim · model-written summary
Malt extract and ABA medium gave maximum somatic embryogenesis in 56.94% of cultures.
- Taxon
- Kinnow mandarint0
- Treatment
- MS medium supplemented with malt extract (500 mg L-1) and ABA (7.56 μM)e0
- Outcome
- somatic embryogenesise0
- Direction
- maximume0
- Value
- 56.94%e0
Not reportedComparator
Verbatim
e0“somatic embryogenesis with a maximum of 56.94% cultures in MS medium supplemented with malt extract (500 mg L-1) and ABA (7.56 μM)”
t0“Kinnow Mandarin”
Claim · model-written summary
Half strength MS medium gave the maximum complete plantlet development of 72.22%.
- Taxon
- Kinnow mandarint0
- Treatment
- ½ MS mediume0
- Outcome
- complete plantletse0
- Direction
- maximume0
- Value
- 72.22%e0
Not reportedComparator
Verbatim
e0“developed into complete plantlets in maximum of 72.22% cultures on ½ MS medium”
t0“Kinnow Mandarin”
Claim · model-written summary
Acclimatized plantlets tested negative for ICRSV.
Verbatim
e0“The plantlets were successfully acclimatizated, transferred to screen house and indexed for ICRSV employing indirect ELISA and RT-PCR and all were found negative of virus”
t0“Kinnow Mandarin”
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