Paper · 2003 · Biotechnology & Biotechnological Equipment

Effect of Cotyledon Age and Explant Location on Regeneration ofCucumis Sativus

N. Yesim Yalcin-Mendi

8
claims
16
verbatim quotes
5
citations
1
research areas
✓
Open access

Abstract

The effects of different cotyledon ages (6, 15, 21 hours and 3 day), cotyledon positions (distal and proximal parts of the cotyledon) and growth regulators (BA and zeatin) on regeneration were tested for swelling and shoot formation.The majority of explants in all treatments (70-94 %) showed evidence of swelling at 10 days.Shoot formation was observed at the 20 1 /z day.Younger seeds gave a significantly higher regeneration than older seeds on shoot formation.Shoot formation ratio was similar in 6, 15 and 21 h.age explants, but the ratio for 3 days old seeds was the worst one.Proximal explants of cotyledons from younger seeds (6, 15, 21 h) produced significantly more shoots (2-2.7 shoots/explant) than distal e).plants (0.75-1.5 shoots/explant).Proximal parts of the older cotyledons (3 days) produced more shoots (0.75 shoots/explant) than distal parts (0.25 shoots I explant).There were no significallf difference between BA and zeatin on shoot formation of regeneration.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Observationobservedoriginal resultmeasured

Claim · model-written summary

Most explants swelled by day 10 across treatments.

Taxon
Cucumis SativusCucumis sativust0
Organ / stage
explantse0
Outcome
swellinge0
Value
70-94 %e0
Qualifier
at 10 dayse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Cucumis Sativus”

✓ located verbatim in the source · title, characters 63–78 · show in text ↑
e0

“The majority of explants in all treatments (70-94 %) showed evidence of swelling at 10 days”

✓ located verbatim in the source · abstract, characters 233–324 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Shoot formation was observed at about day 20.

Taxon
Cucumis SativusCucumis sativust0
Organ / stage
20 1 /z daye0
Outcome
Shoot formatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Cucumis Sativus”

✓ located verbatim in the source · title, characters 63–78 · show in text ↑
e0

“Shoot formation was observed at the 20 1 /z day”

✓ located verbatim in the source · abstract, characters 325–372 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Younger seeds regenerated shoots more than older seeds.

Taxon
Cucumis SativusCucumis sativust0
Material
Younger seedse0
Outcome
regeneratione0
Direction
highere0
Comparator
older seedse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Cucumis Sativus”

✓ located verbatim in the source · title, characters 63–78 · show in text ↑
e0

“Younger seeds gave a significantly higher regeneration than older seeds on shoot formation”

✓ located verbatim in the source · abstract, characters 373–463 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

The shoot formation ratio was similar for 6, 15 and 21 hour explants.

Taxon
Cucumis SativusCucumis sativust0
Organ / stage
explantse0
Outcome
Shoot formation ratioe0
Direction
similare0
Qualifier
6, 15 and 21 h.agee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Cucumis Sativus”

✓ located verbatim in the source · title, characters 63–78 · show in text ↑
e0

“Shoot formation ratio was similar in 6, 15 and 21 h.age explants”

✓ located verbatim in the source · abstract, characters 464–528 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Three-day-old seeds had the lowest shoot formation ratio.

Taxon
Cucumis SativusCucumis sativust0
Material
3 days old seedse0
Outcome
ratioe0
Direction
worste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Cucumis Sativus”

✓ located verbatim in the source · title, characters 63–78 · show in text ↑
e0

“but the ratio for 3 days old seeds was the worst one”

✓ located verbatim in the source · abstract, characters 530–582 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Proximal explants from younger seeds produced more shoots than distal explants.

Taxon
Cucumis SativusCucumis sativust0
Organ / stage
Proximal explants of cotyledonse0
Outcome
shootse0
Direction
moree0
Value
2-2.7 shoots/explante0
Qualifier
0.75-1.5 shoots/explante0
Comparator
distal e).plantse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Cucumis Sativus”

✓ located verbatim in the source · title, characters 63–78 · show in text ↑
e0

“Proximal explants of cotyledons from younger seeds (6, 15, 21 h) produced significantly more shoots (2-2.7 shoots/explant) than distal e).plants (0.75-1.5 shoots/explant)”

✓ located verbatim in the source · abstract, characters 583–753 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Proximal parts of older cotyledons produced more shoots than distal parts.

Taxon
Cucumis SativusCucumis sativust0
Organ / stage
Proximal parts of the older cotyledonse0
Outcome
shootse0
Direction
moree0
Value
0.75 shoots/explante0
Qualifier
0.25 shoots I explante0
Comparator
distal partse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Cucumis Sativus”

✓ located verbatim in the source · title, characters 63–78 · show in text ↑
e0

“Proximal parts of the older cotyledons (3 days) produced more shoots (0.75 shoots/explant) than distal parts (0.25 shoots I explant)”

✓ located verbatim in the source · abstract, characters 754–886 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

BA and zeatin did not differ significantly in shoot formation.

Taxon
Cucumis SativusCucumis sativust0
Treatment
BAe0
Outcome
shoot formatione0
Direction
no significallf differencee0
Comparator
zeatine0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Cucumis Sativus”

✓ located verbatim in the source · title, characters 63–78 · show in text ↑
e0

“There were no significallf difference between BA and zeatin on shoot formation of regeneration”

✓ located verbatim in the source · abstract, characters 887–981 · show in text ↑

N-benzyladenine · growth · Cucumis sativus (cucumber)Results differ

34 papers: 30 positive, 4 negative or no effect

3 papers in the same direction as this paper · 30 reporting no difference from an alternative

  1. positive
  2. positive
  3. positive

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.