Abstract
The inside of Pinus thunbergii could be a reliable screening source for a useful agent in controlling plant disease. Isolation of endophytic actinomycetes from P. thunbergii and their potential as biocontrol agents against the plant pathogen Cylindrocladium sp. were investigated. Two endophytic actinomycetes, Streptomyces sp. and Microbispora sp., were isolated from surface-sterilised root tissues of P. thunbergii seedlings. The recovery test of these two endophytic actinomycetes from pine seedling showed that Streptomyces sp. was isolated from only roots, but Microbispora sp. was isolated from both roots and leaves. Thus, Microbispora sp. is able to move to leaves from roots. Moreover, we evaluated the potential of both strains as biocontrol agents against Cylindrocladium sp. Two weeks after inoculation of Cylindrocladium sp. alone, pine seedlings showed a 50% mortality rate. Co-inoculation of Cylindrocladium sp. and Microbispora sp. did not affect seedling mortality rate. However, inoculation with both Cylindrocladium sp. and Streptomyces sp. reduced seedling mortality to 12%. Streptomyces sp. could be a useful agent in controlling pine disease caused by Cylindrocladium sp. Thus, it seems that Streptomyces sp. may induce a local host defence reaction and Microbispora sp. systemically spreads to aerial parts through the transpiration stream.
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Claims 7
Claim · model-written summary
Co-inoculation with Streptomyces sp. reduced pine seedling mortality to 12%, compared with Cylindrocladium sp. alone (50% mortality).
- Taxon
- Pinus thunbergii
Pinus thunbergiit0 - Treatment
- inoculation with both Cylindrocladium sp. and Streptomyces sp.e5
- Interactor
- Cylindrocladium sp.
Calonectriae5pathogen - Second interactor
- Streptomyces sp.
Streptomyces sp.e5biocontrol_agent - Outcome
- seedling mortalitye5
- Direction
- reducede5
- Value
- 12%e5
- Qualifier
- 50% mortality ratee3
- Comparator
- Cylindrocladium sp. alonee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pinus thunbergii”
e5“inoculation with both Cylindrocladium sp. and Streptomyces sp. reduced seedling mortality to 12%”
e3“Two weeks after inoculation of Cylindrocladium sp. alone, pine seedlings showed a 50% mortality rate”
Claim · model-written summary
Co-inoculation with Microbispora sp. did not affect pine seedling mortality compared with Cylindrocladium sp. alone.
- Taxon
- Pinus thunbergiiPinus thunbergiit0
- Treatment
- Co-inoculation of Cylindrocladium sp. and Microbispora sp.e4
- Interactor
- Cylindrocladium sp.Calonectriae4pathogen
- Second interactor
- Microbispora sp.Microbisporae4biocontrol_agent
- Outcome
- seedling mortality ratee4
- Direction
- did not affecte4
- Comparator
- Cylindrocladium sp. alonee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pinus thunbergii”
e4“Co-inoculation of Cylindrocladium sp. and Microbispora sp. did not affect seedling mortality rate”
e3“Two weeks after inoculation of Cylindrocladium sp. alone, pine seedlings showed a 50% mortality rate”
Claim · model-written summary
Two weeks after inoculation with Cylindrocladium sp. alone, pine seedlings showed 50% mortality.
- Taxon
- Pinus thunbergiiPinus thunbergiit0
- Treatment
- inoculation of Cylindrocladium sp. alonee3
- Interactor
- Cylindrocladium sp.Calonectriae3pathogen
- Outcome
- mortality ratee3
- Value
- 50%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pinus thunbergii”
e3“Two weeks after inoculation of Cylindrocladium sp. alone, pine seedlings showed a 50% mortality rate”
Claim · model-written summary
Streptomyces sp. was isolated from roots only.
- Taxon
- Pinus thunbergiiPinus thunbergiit0
- Organ / stage
- rootse2
- Interactor
- Streptomyces sp.Streptomyces sp.e2symbiont
- Outcome
- isolated from only rootse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pinus thunbergii”
e2“Streptomyces sp. was isolated from only roots, but Microbispora sp. was isolated from both roots and leaves”
Claim · model-written summary
Microbispora sp. was isolated from both roots and leaves of pine seedlings.
- Taxon
- Pinus thunbergiiPinus thunbergiit0
- Organ / stage
- roots and leavese2
- Interactor
- Microbispora sp.Microbisporae2symbiont
- Outcome
- isolated from both roots and leavese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pinus thunbergii”
e2“Streptomyces sp. was isolated from only roots, but Microbispora sp. was isolated from both roots and leaves”
Claim · model-written summary
Two endophytic actinomycetes were isolated from surface-sterilised root tissues of pine seedlings.
- Taxon
- Pinus thunbergiiPinus thunbergiit0
- Organ / stage
- root tissuese1
- Interactor
- Streptomyces sp.Streptomyces sp.e1symbiont
- Second interactor
- Microbispora sp.Microbisporae1symbiont
- Outcome
- isolatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pinus thunbergii”
e1“Two endophytic actinomycetes, Streptomyces sp. and Microbispora sp., were isolated from surface-sterilised root tissues of P. thunbergii seedlings”
Claim · model-written summary
Streptomyces sp. could be a useful agent for controlling pine disease caused by Cylindrocladium sp.
- Interactor
- Cylindrocladium sp.Calonectriae6pathogen
- Second interactor
- Streptomyces sp.Streptomyces sp.e6biocontrol_agent
- Outcome
- controlling pine diseasee6
PinusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pinus thunbergii”
e6“Streptomyces sp. could be a useful agent in controlling pine disease caused by Cylindrocladium sp.”
The same questions in other papers
inoculation · pest · Pinus thunbergii (Japanese black pine)Positive and negative results
1 in the opposite direction
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.