Abstract
A total of 828 isolates of fluorescent pseudomonads (FPs) were obtained from the leaves (305 isolates) and roots (523 isolates) of potato plants grown in different geographical locations in Japan, and 16S rRNA gene sequences of 776 isolates were successfully determined by direct PCR sequencing. Clustering analysis (≥99% identity) identified 13 and 26 operational taxonomic units (OTUs) for leaf- and root-associated FPs, respectively, and 29 OTUs were identified in the phytosphere of potato plants. Among them, 7 and 9 OTUs showed a significantly biased distribution to the leaves and roots, respectively. Phylogenetic analysis revealed that 3 dominant OTUs for leaf-associated FPs were grouped in a cluster of leaf-associated pathogens, such as Pseudomonas cichorii and Pseudomonas viridiflava. In contrast, 4 OTUs were located in a cluster of saprophytic pseudomonads. Among them, 3 OTUs showed high similarity to Pseudomonas koreensis and Pseudomonas vancouverensis, both of which have been reported to be beneficial for biological control or plant growth promotion. These data provide key information for efficient surveying and utilization of beneficial FPs in agricultural practices.
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Claims 10
Claim · model-written summary
The survey recovered 305 leaf and 523 root fluorescent pseudomonad isolates.
- Taxon
- potato plants
Solanum tuberosume0 - Organ / stage
- leavese0
- Interactor
- fluorescent pseudomonadsPseudomonas fluorescens groupe0other
- Outcome
- fluorescent pseudomonads (FPs)e0
- Value
- 828 isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 828 isolates of fluorescent pseudomonads (FPs) were obtained from the leaves (305 isolates) and roots (523 isolates) of potato plants”
Claim · model-written summary
Sequencing succeeded for 776 isolates.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Interactor
- Fluorescent PseudomonadsPseudomonas fluorescens groupt1other
- Outcome
- 16S rRNA gene sequencese0
- Value
- 776 isolatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“16S rRNA gene sequences of 776 isolates were successfully determined by direct PCR sequencing”
t0“Potato Plants”
t1“Fluorescent Pseudomonads”
Claim · model-written summary
Leaf associated fluorescent pseudomonads formed 13 OTUs.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Organ / stage
- leafe0
- Interactor
- Fluorescent PseudomonadsPseudomonas fluorescens groupt1other
- Outcome
- operational taxonomic units (OTUs)e0
- Value
- 13e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified 13 and 26 operational taxonomic units (OTUs) for leaf- and root-associated FPs, respectively”
t0“Potato Plants”
t1“Fluorescent Pseudomonads”
Claim · model-written summary
Root associated fluorescent pseudomonads formed 26 OTUs.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Organ / stage
- roote0
- Interactor
- Fluorescent PseudomonadsPseudomonas fluorescens groupt1other
- Outcome
- operational taxonomic units (OTUs)e0
- Value
- 26e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified 13 and 26 operational taxonomic units (OTUs) for leaf- and root-associated FPs, respectively”
t0“Potato Plants”
t1“Fluorescent Pseudomonads”
Claim · model-written summary
The potato phytosphere contained 29 OTUs.
- Taxon
- potato plantsSolanum tuberosume0
- Interactor
- Fluorescent PseudomonadsPseudomonas fluorescens groupt0other
- Outcome
- OTUse0
- Value
- 29 OTUse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“29 OTUs were identified in the phytosphere of potato plants”
t0“Fluorescent Pseudomonads”
Claim · model-written summary
Seven OTUs were significantly biased toward leaves.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Organ / stage
- leavese0
- Interactor
- Fluorescent PseudomonadsPseudomonas fluorescens groupt1other
- Outcome
- biased distribution to the leavese0
- Value
- 7e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7 and 9 OTUs showed a significantly biased distribution to the leaves and roots, respectively”
t0“Potato Plants”
t1“Fluorescent Pseudomonads”
Claim · model-written summary
Nine OTUs were significantly biased toward roots.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Organ / stage
- rootse0
- Interactor
- Fluorescent PseudomonadsPseudomonas fluorescens groupt1other
- Outcome
- biased distribution to the leaves and rootse0
- Value
- 9e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“7 and 9 OTUs showed a significantly biased distribution to the leaves and roots, respectively”
t0“Potato Plants”
t1“Fluorescent Pseudomonads”
Claim · model-written summary
Three dominant leaf OTUs clustered with leaf associated pathogens.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Interactor
- Pseudomonas cichorii
.jpg?width=60)
Pseudomonas cichoriie0pathogen - Second interactor
- Pseudomonas viridiflava
Pseudomonas viridiflavae0pathogen - Outcome
- cluster of leaf-associated pathogense0
- Value
- 3 dominant OTUse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“3 dominant OTUs for leaf-associated FPs were grouped in a cluster of leaf-associated pathogens, such as Pseudomonas cichorii and Pseudomonas viridiflava”
t0“Potato Plants”
Claim · model-written summary
Four OTUs clustered with saprophytic pseudomonads.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Interactor
- saprophytic pseudomonadse0other
- Outcome
- cluster of saprophytic pseudomonadse0
- Value
- 4 OTUse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“4 OTUs were located in a cluster of saprophytic pseudomonads”
t0“Potato Plants”
Claim · model-written summary
Three OTUs resembled Pseudomonas koreensis and Pseudomonas vancouverensis.
- Taxon
- Potato PlantsSolanum tuberosumt0
- Interactor
- Pseudomonas koreensisPseudomonas koreensise0other
- Second interactor
- Pseudomonas vancouverensisPseudomonas vancouverensise0other
- Outcome
- high similaritye0
- Value
- 3 OTUse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“3 OTUs showed high similarity to Pseudomonas koreensis and Pseudomonas vancouverensis”
t0“Potato Plants”