Pseudomonas cichorii 272 claims

Claim · model-written summary

Isolate 16S sequences shared 99% similarity with Pseudomonas cichorii.

Taxon

Marigoldt0

Interactor

Pseudomonas cichoriiPseudomonas cichoriie0pathogen

Outcome

similarity with those of Pseudomonas cichoriie0

Value

99%e0

Verbatim

e0

“the sequences (GenBank Accession nos. PX664326-PX664329) shared 99% similarity with those of Pseudomonas cichorii”

t0

“Marigold ( Tagetes erecta L.)”

Claim · model-written summary

All four isolates clustered with reference P. cichorii strains with 100% support.

Taxon

Marigoldt0

Interactor

P. cichoriiPseudomonas cichoriie0pathogen

Outcome

all four strains clustered with P. cichorii PPST50936 and P. cichorii DSM50259e0

Value

100%e0

Verbatim

e0

“MLSA indicated that all four strains clustered with P. cichorii PPST50936 and P. cichorii DSM50259 with support rate 100%”

t0

“Marigold ( Tagetes erecta L.)”

Claim · model-written summary

Inoculated marigold leaves developed field-like leaf spots after five days, whereas controls had no symptoms.

Taxon

marigolde1

Treatment

bacterial suspension (OD600=0.6)e1

Interactor

P. cichoriiPseudomonas cichoriie2pathogen

Outcome

leaf spot symptomse0

Comparator

the controle0

Setting

greenhousee1

Not reportedDirection

Verbatim

e0

“Five days after inoculation, leaf spot symptoms similar to those in the field were observed, while the control showed no symptoms”

e1

“bacterial suspension (OD600=0.6) was sprayed on needle punctured leaves of 6-weeks-old marigold at greenhouse”

e2

“P. cichorii was re-isolated from inoculated leaves but not from controls”

Claim · model-written summary

P. cichorii was reisolated from inoculated leaves but absent from controls.

Taxon

Marigoldt0

Interactor

P. cichoriiPseudomonas cichoriie0pathogen

Outcome

P. cichorii was re-isolated from inoculated leaves but not from controlse0

Verbatim

e0

“P. cichorii was re-isolated from inoculated leaves but not from controls”

t0

“Marigold ( Tagetes erecta L.)”

Claim · model-written summary

This was reported as the first P. cichorii leaf spot on marigold in China.

Taxon

Marigolde0

Interactor

P. cichoriiPseudomonas cichoriie0pathogen

Outcome

leaf spote0

Setting

Chinae0

Verbatim

e0

“this is the first report of P. cichorii causing leaf spot on Marigold (T. erecta L.) in China”

Claim · model-written summary

Across eight surveyed fields, bacterial leaf disease incidence on tobacco ranged from 7.5% to 36%.

Taxon

TobaccoNicotiana tabacumt0

Interactor

Pseudomonas cichoriiPseudomonas cichoriit0pathogen

Outcome

disease incidencee0

Value

7.5% to 36%e0

Setting

eight surveyed fieldse0

Verbatim

e0

“Across eight surveyed fields, disease incidence ranged from 7.5% to 36%”

t0

“First Report of Bacterial Leaf Spot on Tobacco Caused by Pseudomonas cichorii in Fujian Province, China”

Claim · model-written summary

The 16S rRNA sequences of the isolates showed 99.5-100% identity to Pseudomonas cichorii strains.

Taxon

TobaccoNicotiana tabacumt0

Interactor

Pseudomonas cichoriiPseudomonas cichoriie0pathogen

Gene

16S rRNA sequencese1

Outcome

sequence identitye0

Value

99.5-100%e0

Verbatim

e0

“showed 99.5-100% sequence identity to Pseudomonas cichorii strains”

e1

“The 16S rRNA sequences were deposited in GenBank”

t0

“First Report of Bacterial Leaf Spot on Tobacco Caused by Pseudomonas cichorii in Fujian Province, China”

Claim · model-written summary

gyrB and rpoD sequences of the isolates exhibited more than 99% nucleotide identity to known P. cichorii strains.

Taxon

TobaccoNicotiana tabacumt0

Interactor

P. cichoriiPseudomonas cichoriie0pathogen

Gene

gyrB and rpoDe1

Outcome

nucleotide identitye0

Value

>99%e0

Verbatim

e0

“The resulting sequences exhibited >99% nucleotide identity to those of known P. cichorii strains in the GenBank database”

e1

“Multilocus sequence analysis (MLSA) was performed on the housekeeping genes gyrB and rpoD”

t0

“First Report of Bacterial Leaf Spot on Tobacco Caused by Pseudomonas cichorii in Fujian Province, China”

Claim · model-written summary

Maximum likelihood and Bayesian trees placed isolates FJSMCC21 and FJSMCC37 within the P. cichorii monophyletic clade with strong support.

Taxon

TobaccoNicotiana tabacumt0

Material

FJSMCC21 and FJSMCC37e0

Interactor

P. cichoriiPseudomonas cichoriie0pathogen

Outcome

monophyletic cladee0

Value

ML bootstrap = 100%e0

Verbatim

e0

“Both topologies placed isolates FJSMCC21 and FJSMCC37 within the P. cichorii monophyletic clade with strong support (ML bootstrap = 100%; BI posterior probability = 1)”

t0

“First Report of Bacterial Leaf Spot on Tobacco Caused by Pseudomonas cichorii in Fujian Province, China”

Claim · model-written summary

Needle-puncture inoculation of healthy tobacco with a bacterial suspension produced water-soaked spots within one day and field-like symptoms on all inoculated leaves within five days, while water-inoculated controls remained asymptomatic.

Taxon

TobaccoNicotiana tabacumt0

Treatment

Bacterial suspension (1×10⁸ CFU/mL)e1

Interactor

Pseudomonas cichoriiPseudomonas cichoriit0pathogen

Outcome

field-like symptomse0

Comparator

sterile watere2

Not reportedDirection

Verbatim

e0

“all inoculated leaves developed field-like symptoms within five days, while controls remained asymptomatic”

e1

“Bacterial suspension (1×10⁸ CFU/mL) was aseptically needle-punctured into five leaves”

e2

“Controls were inoculated with sterile water”

t0

“First Report of Bacterial Leaf Spot on Tobacco Caused by Pseudomonas cichorii in Fujian Province, China”