Abstract
MS배지와 PM (Potato medium)배지 (MS배지에서 $NH_4NO_3$ 와 $KNO_3$의 양은 1/2, $CaCl_2$는 2배 첨가하고 나머지는 동일)에서 대서, 자심, 추백, 하령 4 품종 감자줄기의 기내 대량증식을 위해 실험하였다. 고체배지에서 배양초기 ($1{\sim}2$주)에 대서와 자심은 MS배지에서 줄기의 신장이 가장 우수하였고, 하령과 추백은 PM배지가 효과적이었지만 배양후기인 4주 째에는 두 배지 사이에 차이가 없었다. 액체 정치배양한 배지에서는 고체배지와 달리 배양초기에는 생장이 늦었고 배양 1주 후부터 감자 (4 cm 이상)줄기 발생률이 급격히 증가하기 시작하였고, 4주까지 꾸준히 생장을 지속하였다. 바이오리액터에서 줄기의 생장은 품종에 따라 차이를 보였다. 특히 추백과 자심은 액아 발생뿐만 아니라 줄기신장이 1/4 PM배지가 우수하였고, 대서는 1/2 PM배지에서 액아발생이 왕성하였다. 한편, 하령은 다른 품종에 비해 생장이 늦었다. A study was conducted to investigate in vitro micro propagation of four potato cultivars of Daese, Jasim, Chubaek and Haryeng in MS medium and PM medium (a medium containing half concentration of $NH_4NO_3$ and $KNO_3$, two fold concentration of $CaCl_2$ as compared to MS medium). During $1{\sim}2$ weeks of culture, Daese and Jasim showed better shoot elongation on the solid MS medium than Chubaek and Haryeng whereas Chubaek and Haryeng did better shoot elongation on the solid PM medium. But no difference was observed after 4 weeks of culture. As compared to shoot elongation on the solid medium, it was delayed at early stage of culture in the liquid medium without shaking. Shoot formation ratio of potato (above 4 cm of shoot length) began to increase significantly after 1 week of culture and kept on increasing until 4 weeks. The four cultivars showed the different patterns of shoot growth in bioreactor. The PM medium with a quarter salt strength was effective for the regeneration of axillary buds as well as shoot elongation of Jasim and Chubaek. Daese showed vigorous regeneration of axillary buds in the PM medium with a half salt strength. On the other hand, Haryeng showed slower growth than the other three cultivars.
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Claims 9
Claim · model-written summary
Daese and Jasim showed better early shoot elongation on solid MS medium.
- Taxon
- potato
Solanum tuberosume0 - Material
- Daese and Jasime1
- Treatment
- solid MS mediume1
- Outcome
- shoot elongatione1
- Direction
- bettere1
- Comparator
- Chubaek and Haryenge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato cultivars of Daese, Jasim, Chubaek and Haryeng”
e1“During $1{\sim}2$ weeks of culture, Daese and Jasim showed better shoot elongation on the solid MS medium than Chubaek and Haryeng”
Claim · model-written summary
Chubaek and Haryeng showed better early shoot elongation on solid PM medium.
- Taxon
- potatoSolanum tuberosume0
- Material
- Chubaek and Haryenge1
- Treatment
- solid PM mediume1
- Outcome
- shoot elongatione1
- Direction
- bettere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato cultivars of Daese, Jasim, Chubaek and Haryeng”
e1“Chubaek and Haryeng did better shoot elongation on the solid PM medium”
Claim · model-written summary
The media showed no difference after four weeks.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- differencee1
- Direction
- no differencee1
- Qualifier
- after 4 weeks of culturee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato cultivars of Daese, Jasim, Chubaek and Haryeng”
e1“no difference was observed after 4 weeks of culture”
Claim · model-written summary
Shoot elongation was delayed early in static liquid culture relative to solid medium.
- Taxon
- potatoSolanum tuberosume0
- Treatment
- liquid medium without shakinge1
- Outcome
- shoot elongatione1
- Direction
- delayede1
- Comparator
- solid mediume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato cultivars of Daese, Jasim, Chubaek and Haryeng”
e1“As compared to shoot elongation on the solid medium, it was delayed at early stage of culture in the liquid medium without shaking”
Claim · model-written summary
Potato shoot formation ratio rose after one week and continued rising to four weeks.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- Shoot formation ratioe0
- Direction
- increasee0
- Qualifier
- above 4 cm of shoot lengthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot formation ratio of potato (above 4 cm of shoot length) began to increase significantly after 1 week of culture and kept on increasing until 4 weeks”
Claim · model-written summary
The four cultivars differed in shoot growth patterns in the bioreactor.
- Taxon
- potatoSolanum tuberosume0
- Outcome
- different patterns of shoot growthe1
- Setting
- bioreactore1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato cultivars of Daese, Jasim, Chubaek and Haryeng”
e1“The four cultivars showed the different patterns of shoot growth in bioreactor”
Claim · model-written summary
Quarter-strength PM medium effectively regenerated axillary buds and elongated shoots of Jasim and Chubaek.
- Taxon
- potatoSolanum tuberosume0
- Material
- Jasim and Chubaeke1
- Treatment
- PM medium with a quarter salt strengthe1
- Outcome
- regeneration of axillary buds as well as shoot elongatione1
- Direction
- effectivee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato cultivars of Daese, Jasim, Chubaek and Haryeng”
e1“The PM medium with a quarter salt strength was effective for the regeneration of axillary buds as well as shoot elongation of Jasim and Chubaek”
Claim · model-written summary
Daese showed vigorous axillary bud regeneration on half-strength PM medium.
- Taxon
- potatoSolanum tuberosume0
- Material
- Daesee1
- Treatment
- PM medium with a half salt strengthe1
- Outcome
- regeneration of axillary budse1
- Direction
- vigorouse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato cultivars of Daese, Jasim, Chubaek and Haryeng”
e1“Daese showed vigorous regeneration of axillary buds in the PM medium with a half salt strength”
Claim · model-written summary
Haryeng grew more slowly than the other three cultivars.
- Taxon
- potatoSolanum tuberosume0
- Material
- Haryenge1
- Outcome
- growthe1
- Direction
- slowere1
- Comparator
- the other three cultivarse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“potato cultivars of Daese, Jasim, Chubaek and Haryeng”
e1“Haryeng showed slower growth than the other three cultivars”
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See all 82 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.