Abstract
A liquid chromatography-mass spectrometry (LC/MS) method for determining the enzymatic activity of 5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase), an enzyme of the shikimate pathway, was developed. EPSP synthase catalyzes the formation of 5-enolpyruvylshikimate-3-phosphate (EPSP) from shikimate-3-phosphate (S-3-P) and phosphoenolpyruvate (PEP) in microorganisms and plants. The enzymatic activity of EPSP synthase was assessed by the determination of EPSP after a 30-min incubation with S-3-P and PEP using the LC/MS system. EPSP synthase activity is given in terms of the produced EPSP (pmol/min/mg protein). Glyphosate (N-phosphonomethyl glycine)-tolerant EPSP synthase from the Agrobacterium sp. strain CP4 (CP4-EPSP synthase) in genetically modified soybeans (GM-soybeans) was found to have an enzymatic activity of 736 EPSP pmol/min/mg protein in the presence of 3 nmol of S-3-P. In contrast, the enzyme activity of non-GM-soybeans was 21 EPSP pmol/min/mg protein. The EPSP synthase activity was markedly decreased in the non-GM-soybeans by the addition of glyphosate, but the enzyme activity of the GM-soybeans was only slightly decreased with this treatment. This LC/MS system could also be applicable to the measurement of EPSP synthase activity in different plant species and the detection of herbicide-tolerant EPSP synthase in GM foods.
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Claims 4
Claim · model-written summary
CP4 EPSP synthase activity in GM soybeans was 736 EPSP pmol/min/mg protein with 3 nmol S-3-P.
- Taxon
- soybeans
Glycine maxe0 - Material
- GM-soybeanse0
- Gene
- CP4-EPSP synthasee0
- Outcome
- enzymatic activitye0
- Value
- 736 EPSP pmol/min/mg proteine0
- Qualifier
- 3 nmol of S-3-Pe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CP4-EPSP synthase) in genetically modified soybeans (GM-soybeans) was found to have an enzymatic activity of 736 EPSP pmol/min/mg protein in the presence of 3 nmol of S-3-P”
Claim · model-written summary
EPSP synthase activity in non-GM soybeans was 21 EPSP pmol/min/mg protein.
- Taxon
- soybeansGlycine maxe0
- Material
- non-GM-soybeanse0
- Outcome
- enzyme activitye0
- Value
- 21 EPSP pmol/min/mg proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the enzyme activity of non-GM-soybeans was 21 EPSP pmol/min/mg protein”
Claim · model-written summary
Glyphosate markedly decreased EPSP synthase activity in non-GM soybeans.
- Taxon
- soybeansGlycine maxe0
- Material
- non-GM-soybeanse0
- Treatment
- addition of glyphosatee0
- Outcome
- EPSP synthase activitye0
- Direction
- markedly decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EPSP synthase activity was markedly decreased in the non-GM-soybeans by the addition of glyphosate”
Claim · model-written summary
Glyphosate only slightly decreased EPSP synthase activity in GM soybeans.
- Taxon
- soybeansGlycine maxe0
- Material
- GM-soybeanse0
- Treatment
- glyphosatee1
- Gene
- EPSP synthasee2
- Outcome
- enzyme activitye0
- Direction
- only slightly decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the enzyme activity of the GM-soybeans was only slightly decreased with this treatment”
e1“addition of glyphosate”
e2“EPSP synthase activity was markedly decreased in the non-GM-soybeans”
The same questions in other papers
glyphosate · soil: biology · Glycine max (soybean)Positive and negative results
3 papers in the same direction as this paper · 8 in the opposite direction
- negative
- no effect
- no effect
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See all 12 papers →glyphosate · physiology: enzyme activity · Glycine max (soybean)Results agree
4 papers in the same direction as this paper
- negative
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.