Abstract
Many agricultural uses of bacteria require the establishment of efficient bacterial populations in the rhizosphere, for which colonization of plant seeds often constitutes a critical first step. Pseudomonas putida KT2440 is a strain that colonizes the rhizosphere of a number of agronomically important plants at high population densities. To identify the functions involved in initial seed colonization by P. putida KT2440, we subjected this strain to transposon mutagenesis and screened for mutants defective in attachment to corn seeds. Eight different mutants were isolated and characterized. While all of them showed reduced attachment to seeds, only two had strong defects in their adhesion to abiotic surfaces (glass and different plastics). Sequences of the loci affected in all eight mutants were obtained. None of the isolated genes had previously been described in P. putida, although four of them showed clear similarities with genes of known functions in other organisms. They corresponded to putative surface and membrane proteins, including a calcium-binding protein, a hemolysin, a peptide transporter, and a potential multidrug efflux pump. One other showed limited similarities with surface proteins, while the remaining three presented no obvious similarities with known genes, indicating that this study has disclosed novel functions.
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Claims 7
Claim · model-written summary
All eight transposon mutants of Pseudomonas putida KT2440 isolated in a screen for attachment defects showed reduced attachment to corn seeds.
- Taxon
- corn
Zea mays subsp. mayse1 - Material
- mutantse1
- Interactor
- Pseudomonas putida
Pseudomonas putidat0other - Outcome
- attachment to seedse0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“While all of them showed reduced attachment to seeds”
e1“screened for mutants defective in attachment to corn seeds”
t0“Pseudomonas putida”
Claim · model-written summary
Only two of the eight P. putida mutants had strong defects in adhesion to abiotic surfaces such as glass and different plastics.
- Interactor
- Pseudomonas putidaPseudomonas putidat0other
- Outcome
- adhesion to abiotic surfacese0
- Qualifier
- only two had strong defectse0
- Setting
- glass and different plasticse0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only two had strong defects in their adhesion to abiotic surfaces (glass and different plastics)”
t0“Pseudomonas putida”
Claim · model-written summary
None of the isolated genes had previously been described in P. putida, although four showed clear similarities with genes of known function in other organisms.
- Interactor
- Pseudomonas putidaPseudomonas putidat0other
- Gene
- isolated genese0
- Outcome
- None of the isolated genes had previously been described in P. putidae0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“None of the isolated genes had previously been described in P. putida, although four of them showed clear similarities with genes of known functions in other organisms”
t0“Pseudomonas putida”
Claim · model-written summary
Four of the affected genes corresponded to putative surface and membrane proteins including a calcium-binding protein, a hemolysin and a peptide transporter.
- Interactor
- Pseudomonas putidaPseudomonas putidat0other
- Gene
- a calcium-binding protein, a hemolysin, a peptide transportere0
- Outcome
- putative surface and membrane proteinse0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They corresponded to putative surface and membrane proteins, including a calcium-binding protein, a hemolysin, a peptide transporter, and a potential multidrug efflux pump”
t0“Pseudomonas putida”
Claim · model-written summary
One affected gene corresponded to a potential multidrug efflux pump among putative surface and membrane proteins.
- Interactor
- Pseudomonas putidaPseudomonas putidat0other
- Gene
- a potential multidrug efflux pumpe0
- Outcome
- putative surface and membrane proteinse0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They corresponded to putative surface and membrane proteins, including a calcium-binding protein, a hemolysin, a peptide transporter, and a potential multidrug efflux pump”
t0“Pseudomonas putida”
Claim · model-written summary
One further affected gene showed limited similarities with surface proteins.
- Interactor
- Pseudomonas putidaPseudomonas putidat0other
- Gene
- One othere0
- Outcome
- limited similarities with surface proteinse0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One other showed limited similarities with surface proteins”
t0“Pseudomonas putida”
Claim · model-written summary
The remaining three affected genes presented no obvious similarities with known genes, indicating novel functions.
- Interactor
- Pseudomonas putidaPseudomonas putidat0other
- Gene
- the remaining threee0
- Outcome
- no obvious similarities with known genese0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the remaining three presented no obvious similarities with known genes”
t0“Pseudomonas putida”