Abstract
Ectomycorrhizal inoculation techniques were applied to Douglas fir (Pseudotsuga menziesii (Mirb) Franco) cuttings, dur- ing the rooting phase, to determine the effect of inoculation on rooting and mycorrhizal colonization. Two experiments were con- ducted: 1) an inoculation experiment, established at three different locations with the same treatments, to determine the effect of inoc- ulation with Laccaria bicolor, Melanogaster ambiguus and Rhizopogon subareolatus; and 2) a local factorial experiment to determine the effect of fungal inoculation, substrate disinfection methods, and their possible interactions. At Peyrat-le-Chteau and Champenoux (France), there were no differences in rooting due to the inoculation treatment. At Cabrils (Spain), the cuttings inoculated with L. bicol- or and R. subareolatus rooted significantly better than non-inoculated cuttings. Vegetative inoculum of L. bicolor and spores of R. subareolatus were effective in forming ectomycorrhizas in the three locations, whereas spores of M. ambiguus were effective only at Cabrils. Substrate disinfection with methyl bromide significantly decreased the percentage of rooted cuttings compared to steam-dis- infected substrate. Methyl bromide application also lowered the percentage of mycorrhizal cuttings after inoculation with L. bicolor and R. subareolatus. The disinfection method did not alter significantly the colonization level among the mycorrhizal plants.
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Claims 10
Claim · model-written summary
Inoculation did not alter rooting at two French sites.
- Taxon
- Douglas fir
Pseudotsuga menziesiit0
- Treatment
- inoculation treatmente0
- Outcome
- rootinge0
- Direction
- no differencese0
- Setting
- Peyrat-le-Chteau and Champenoux (France)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At Peyrat-le-Chteau and Champenoux (France), there were no differences in rooting due to the inoculation treatment”
t0“Douglas fir”
Claim · model-written summary
L. bicolor inoculation improved rooting at Cabrils.
- Taxon
- Douglas fir
Pseudotsuga menziesiit0
- Treatment
- inoculated with L. bicol- ore0
- Interactor
- L. bicol- ore0symbiont
- Outcome
- rootede0
- Direction
- bettere0
- Comparator
- non-inoculated cuttingse0
- Setting
- Cabrils (Spain)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At Cabrils (Spain), the cuttings inoculated with L. bicol- or and R. subareolatus rooted significantly better than non-inoculated cuttings”
t0“Douglas fir”
Claim · model-written summary
R. subareolatus inoculation improved rooting at Cabrils.
- Taxon
- Douglas fir
Pseudotsuga menziesiit0
- Treatment
- inoculated with L. bicol- or and R. subareolatuse0
- Interactor
- R. subareolatusRhizopogon subareolatuse0symbiont
- Outcome
- rootede0
- Direction
- bettere0
- Comparator
- non-inoculated cuttingse0
- Setting
- Cabrils (Spain)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At Cabrils (Spain), the cuttings inoculated with L. bicol- or and R. subareolatus rooted significantly better than non-inoculated cuttings”
t0“Douglas fir”
Claim · model-written summary
L. bicolor vegetative inoculum formed ectomycorrhizas at all three locations.
- Taxon
- Douglas fir
Pseudotsuga menziesiit0
- Interactor
- L. bicolor
Laccaria bicolore0symbiont - Outcome
- forming ectomycorrhizase0
- Setting
- three locationse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_P.D._Orton_270921_crop.jpg?width=500)
Verbatim
e0“Vegetative inoculum of L. bicolor and spores of R. subareolatus were effective in forming ectomycorrhizas in the three locations”
t0“Douglas fir”
Claim · model-written summary
R. subareolatus spores formed ectomycorrhizas at all three locations.
- Taxon
- Douglas fir
Pseudotsuga menziesiit0
- Interactor
- R. subareolatusRhizopogon subareolatuse0symbiont
- Outcome
- forming ectomycorrhizase0
- Setting
- three locationse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“spores of R. subareolatus were effective in forming ectomycorrhizas in the three locations”
t0“Douglas fir”
Claim · model-written summary
M. ambiguus spores formed ectomycorrhizas only at Cabrils.
- Taxon
- Douglas fir
Pseudotsuga menziesiit0
- Interactor
- M. ambiguus
Melanogaster ambiguuse0symbiont - Outcome
- forming ectomycorrhizase1
- Setting
- Cabrilse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“spores of M. ambiguus were effective only at Cabrils”
e1“forming ectomycorrhizas”
t0“Douglas fir”
Claim · model-written summary
Methyl bromide disinfection reduced rooting relative to steam disinfection.
- Taxon
- Douglas fir
Pseudotsuga menziesiit0
- Treatment
- Substrate disinfection with methyl bromidee0
- Outcome
- percentage of rooted cuttingse0
- Direction
- decreasede0
- Comparator
- steam-dis- infected substratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Substrate disinfection with methyl bromide significantly decreased the percentage of rooted cuttings compared to steam-dis- infected substrate”
t0“Douglas fir”
Claim · model-written summary
Methyl bromide lowered mycorrhizal cutting percentage after L. bicolor inoculation.
- Taxon
- Douglas fir
Pseudotsuga menziesiit0
- Treatment
- Methyl bromide applicatione0
- Interactor
- L. bicolor
Laccaria bicolore0symbiont - Outcome
- percentage of mycorrhizal cuttingse0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_P.D._Orton_270921_crop.jpg?width=500)
Verbatim
e0“Methyl bromide application also lowered the percentage of mycorrhizal cuttings after inoculation with L. bicolor and R. subareolatus”
t0“Douglas fir”
Claim · model-written summary
Methyl bromide lowered mycorrhizal cutting percentage after R. subareolatus inoculation.
- Taxon
- Douglas fir
Pseudotsuga menziesiit0
- Treatment
- Methyl bromide applicatione0
- Interactor
- R. subareolatusRhizopogon subareolatuse0symbiont
- Outcome
- percentage of mycorrhizal cuttingse0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Methyl bromide application also lowered the percentage of mycorrhizal cuttings after inoculation with L. bicolor and R. subareolatus”
t0“Douglas fir”
Claim · model-written summary
Disinfection method did not significantly alter colonization among mycorrhizal plants.
- Taxon
- Douglas fir
Pseudotsuga menziesiit0
- Treatment
- disinfection methode0
- Outcome
- colonization levele0
- Direction
- did not altere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The disinfection method did not alter significantly the colonization level among the mycorrhizal plants”
t0“Douglas fir”
The same questions in other papers
Rhizopogon subareolatus · growth · Pseudotsuga menziesii (Douglas-fir)Positive and negative results
1 in the opposite direction
- negative
bromomethane · growth · Pseudotsuga menziesii (Douglas-fir)Positive and negative results
2 in the opposite direction
- positive
- positive
inoculation · growth · Pseudotsuga menziesii (Douglas-fir)Positive and negative results
9 papers in the same direction as this paper · 4 in the opposite direction
- negative
- no effect
- no effect
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.