Abstract
Genome packaging in the plant-infecting Brome mosaic virus (BMV), a member of the alphavirus-like superfamily, as well as in other positive-strand RNA viruses pathogenic to humans (e.g., poliovirus) and animals (e.g., Flock House virus), is functionally coupled to replication. Although the subcellular localization site of BMV replication has been identified, that of the capsid protein (CP) has remained elusive. In this study, the application of immunofluorescence confocal microscopy to Nicotiana benthamiana leaves expressing replication-derived BMV CP as a green fluorescent protein (GFP) fusion, in conjunction with antibodies to the CP and double-stranded RNA, a presumed marker of RNA replication, revealed that the subcellular localization sites of replication and CP overlap. Our temporal analysis by transmission electron microscopy of ultrastructural modifications induced in BMV-infected N. benthamiana leaves revealed a reticulovesicular network of modified endoplasmic reticulum (ER) incorporating large assemblies of vesicles derived from ER accumulated in the cytoplasm during BMV infection. Additionally, for the first time, we have found by ectopic expression experiments that BMV CP itself has the intrinsic property of modifying ER to induce vesicles similar to those present in BMV infections. The significance of CP-induced vesicles in relation to CP-organized viral functions that are linked to replication-coupled packaging is discussed.
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Claims 3
Claim · model-written summary
BMV capsid protein and replication localization sites overlapped in Nicotiana leaves.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Interactor
- BMV
Brome mosaic viruse0pathogen - Gene
- BMV CPe0
- Outcome
- subcellular localization sites of replication and CP overlape0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nicotiana benthamiana leaves expressing replication-derived BMV CP as a green fluorescent protein (GFP) fusion, in conjunction with antibodies to the CP and double-stranded RNA, a presumed marker of RNA replication, revealed that the subcellular localization sites of replication and CP overlap.”
Claim · model-written summary
BMV infection induced a modified ER network with vesicle assemblies in Nicotiana leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- BMV infectione0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- reticulovesicular network of modified endoplasmic reticulum (ER)e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BMV-infected N. benthamiana leaves revealed a reticulovesicular network of modified endoplasmic reticulum (ER) incorporating large assemblies of vesicles derived from ER accumulated in the cytoplasm during BMV infection.”
e1“Nicotiana benthamiana leaves”
Claim · model-written summary
Ectopic BMV CP expression induced ER vesicles similar to those in BMV infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- ectopic expressione0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Gene
- BMV CPe0
- Outcome
- vesicles similar to those present in BMV infectionse0
- Comparator
- BMV infectionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“by ectopic expression experiments that BMV CP itself has the intrinsic property of modifying ER to induce vesicles similar to those present in BMV infections.”
e1“Nicotiana benthamiana leaves”
The same questions in other papers
bromine atom · Brome mosaic virus · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.