Abstract
Brome mosaic virus (BMV) RNA replication has been examined in a number of systems, including Saccharomyces cerevisiae. We developed an efficient T-DNA-based gene delivery system using Agrobacterium tumefaciens to transiently express BMV RNAs in Nicotiana benthamiana. The expressed RNAs can systemically infect plants and provide material to extract BMV replicase that can perform template-dependent RNA-dependent RNA synthesis in vitro. We also expressed the four BMV-encoded proteins from nonreplicating RNAs and analyzed their effects on BMV RNA accumulation. The capsid protein that coinfiltrated with constructs expressing RNA1 and RNA2 suppressed minus-strand levels but increased plus-strand RNA accumulation. The replication proteins 1a and 2a could function in trans to replicate and transcribe the BMV RNAs. None of the BMV proteins or RNA could efficiently suppress posttranscriptional silencing. However, 1a expressed in trans will suppress the production of a recombinant green fluorescent protein expressed from the nontranslated portions of BMV RNA1 and RNA2, suggesting that 1a may regulate translation from BMV RNAs. BMV replicase proteins 1a did not affect the accumulation of the BMV RNAs in the absence of RNA replication, unlike the situation reported for S. cerevisiae. This work demonstrates that the Agrobacterium-mediated gene delivery system can be used to study the cis- and trans-acting requirements for BMV RNA replication in plants and that significant differences can exist for BMV RNA replication in different hosts.
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Claims 11
Claim · model-written summary
Expressed BMV RNAs systemically infected plants.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Outcome
- systemically infect plantse0
Not reportedInteractor
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expressed RNAs can systemically infect plants”
e1“in Nicotiana benthamiana”
Claim · model-written summary
The delivered RNAs yielded BMV replicase capable of template-dependent RNA synthesis in vitro.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- Brome Mosaic Virus
Brome mosaic virust0pathogen - Outcome
- template-dependent RNA-dependent RNA synthesise0
- Value
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“provide material to extract BMV replicase that can perform template-dependent RNA-dependent RNA synthesis in vitro”
e1“in Nicotiana benthamiana”
t0“Brome Mosaic Virus”
Claim · model-written summary
Capsid protein suppressed BMV minus-strand levels.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- capsid protein that coinfiltrated with constructs expressing RNA1 and RNA2e0
- Interactor
- Brome Mosaic VirusBrome mosaic virust0pathogen
- Gene
- capsid proteine0
- Outcome
- minus-strand levelse0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The capsid protein that coinfiltrated with constructs expressing RNA1 and RNA2 suppressed minus-strand levels”
e1“in Nicotiana benthamiana”
t0“Brome Mosaic Virus”
Claim · model-written summary
Capsid protein increased BMV plus-strand RNA accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- capsid protein that coinfiltrated with constructs expressing RNA1 and RNA2e0
- Interactor
- Brome Mosaic VirusBrome mosaic virust0pathogen
- Gene
- capsid proteine0
- Outcome
- plus-strand RNA accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The capsid protein that coinfiltrated with constructs expressing RNA1 and RNA2 suppressed minus-strand levels but increased plus-strand RNA accumulation”
e1“in Nicotiana benthamiana”
t0“Brome Mosaic Virus”
Claim · model-written summary
Replication proteins 1a and 2a functioned in trans to replicate and transcribe BMV RNAs.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- BMVBrome mosaic viruse0pathogen
- Gene
- replication proteins 1a and 2ae0
- Outcome
- replicate and transcribe the BMV RNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The replication proteins 1a and 2a could function in trans to replicate and transcribe the BMV RNAs”
e1“in Nicotiana benthamiana”
Claim · model-written summary
No BMV protein or RNA efficiently suppressed posttranscriptional silencing.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- suppress posttranscriptional silencinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“None of the BMV proteins or RNA could efficiently suppress posttranscriptional silencing”
e1“in Nicotiana benthamiana”
Claim · model-written summary
Trans-expressed 1a suppressed recombinant green fluorescent protein production from BMV RNA nontranslated regions.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- 1a expressed in transe0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Gene
- 1ae0
- Outcome
- production of a recombinant green fluorescent proteine0
- Direction
- suppresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1a expressed in trans will suppress the production of a recombinant green fluorescent protein expressed from the nontranslated portions of BMV RNA1 and RNA2”
e1“in Nicotiana benthamiana”
Claim · model-written summary
BMV 1a may regulate translation from BMV RNAs.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- BMVBrome mosaic viruse0pathogen
- Gene
- 1ae0
- Outcome
- translation from BMV RNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that 1a may regulate translation from BMV RNAs”
e1“in Nicotiana benthamiana”
Claim · model-written summary
BMV replicase 1a did not affect BMV RNA accumulation without RNA replication.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- BMV replicase proteins 1ae0
- Interactor
- BMVBrome mosaic viruse0pathogen
- Gene
- BMV replicase proteins 1ae0
- Outcome
- accumulation of the BMV RNAse0
- Direction
- did not affecte0
- Qualifier
- in the absence of RNA replicatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BMV replicase proteins 1a did not affect the accumulation of the BMV RNAs in the absence of RNA replication”
e1“in Nicotiana benthamiana”
Claim · model-written summary
The Agrobacterium delivery system can study cis- and trans-acting requirements of BMV replication in plants.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- study the cis- and trans-acting requirements for BMV RNA replicatione0
- Value
- in plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the Agrobacterium-mediated gene delivery system can be used to study the cis- and trans-acting requirements for BMV RNA replication in plants”
e1“in Nicotiana benthamiana”
Claim · model-written summary
BMV RNA replication can differ significantly among hosts.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- BMVBrome mosaic viruse0pathogen
- Outcome
- BMV RNA replicatione0
- Direction
- differencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant differences can exist for BMV RNA replication in different hosts”
e1“in Nicotiana benthamiana”
The same questions in other papers
bromine atom · Brome mosaic virus · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.