Abstract
Abstract Experiments were performed to determine if RAPD profiles developed using total DNA isolated from soybean seed could be affected by the physiological state or the quality of the seed. RAPD profiles were developed using template DNA isolated from a single seed lot of soybean ( Glycine max L. cv. Kirby). High quality seeds were used to produce four populations varying in either quality or physiological state: untreated control seed ambient temperature and humidity storage for 12 months, accelerated aging at 41°C and 100% relative humidity for 48 h, and controlled hydration (seed priming). One hundred and eighty-eight primers were used to create separate RAPD profiles from total DNA isolated from each set of seed and from soybean leaf tissue. Sixteen polymorphisms from 14 primers were identified as a result of seed treatments. Six primers showed nine polymorphisms in RAPD profiles of ambient-stored seed DNA, while four and two primers produced polymorphisms in reactions using accelerated aged or primed-seed template DNA, respectively. Two primers showed a polymorphic fragment in vegetative DNA not observed in any of the seed DNA samples. Ten of the observed polymorphisms were due to the appearance of a DNA fragment in response to a specific seed treatment while six were the result of the treatment-induced loss of a DNA fragment. The six polymorphisms resulting from the loss of a major fragment were all due to ambient-temperature seed storage. Results were reproducibly obtained from multiple DNA isolations using three separate DNA isolation procedures involving either multiple seed or a single seed as the template source. Therefore, genetically identical seed can consistently display RAPD polymorphisms as a response to the environmental exposure.
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Claims 9
Claim · model-written summary
Seed treatments produced sixteen RAPD polymorphisms from fourteen primers.
- Taxon
- soybean
Glycine maxt0 - Treatment
- seed treatmentse0
- Outcome
- polymorphismse0
- Value
- Sixteen polymorphismse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sixteen polymorphisms from 14 primers were identified as a result of seed treatments”
t0“soybean”
Claim · model-written summary
Ambient-stored seed DNA yielded nine polymorphisms from six primers.
- Taxon
- soybeanGlycine maxt0
- Treatment
- ambient-storede0
- Outcome
- polymorphisms in RAPD profilese0
- Value
- nine polymorphismse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six primers showed nine polymorphisms in RAPD profiles of ambient-stored seed DNA”
t0“soybean”
Claim · model-written summary
Accelerated aged seed DNA yielded polymorphisms with four primers.
- Taxon
- soybeanGlycine maxt0
- Treatment
- accelerated agede0
- Outcome
- polymorphismse0
- Value
- foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four and two primers produced polymorphisms in reactions using accelerated aged or primed-seed template DNA, respectively”
t0“soybean”
Claim · model-written summary
Primed seed DNA yielded polymorphisms with two primers.
- Taxon
- soybeanGlycine maxt0
- Treatment
- primed-seede0
- Outcome
- polymorphismse0
- Value
- twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“four and two primers produced polymorphisms in reactions using accelerated aged or primed-seed template DNA, respectively”
t0“soybean”
Claim · model-written summary
Vegetative DNA had a polymorphic fragment absent from all seed DNA samples with two primers.
- Taxon
- soybeanGlycine maxt0
- Outcome
- polymorphic fragmente0
- Value
- Two primerse0
- Comparator
- seed DNA samplese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Two primers showed a polymorphic fragment in vegetative DNA not observed in any of the seed DNA samples”
t0“soybean”
Claim · model-written summary
Ten polymorphisms reflected appearance of a DNA fragment after seed treatment.
- Taxon
- soybeanGlycine maxt0
- Treatment
- specific seed treatmente0
- Outcome
- appearance of a DNA fragmente0
- Value
- Tene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ten of the observed polymorphisms were due to the appearance of a DNA fragment in response to a specific seed treatment”
t0“soybean”
Claim · model-written summary
Six polymorphisms reflected treatment-induced loss of a DNA fragment.
- Taxon
- soybeanGlycine maxt0
- Outcome
- treatment-induced loss of a DNA fragmente0
- Value
- sixe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“six were the result of the treatment-induced loss of a DNA fragment”
t0“soybean”
Claim · model-written summary
All six major-fragment-loss polymorphisms resulted from ambient-temperature seed storage.
- Taxon
- soybeanGlycine maxt0
- Treatment
- ambient-temperature seed storagee0
- Outcome
- loss of a major fragmente0
- Value
- six polymorphismse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The six polymorphisms resulting from the loss of a major fragment were all due to ambient-temperature seed storage”
t0“soybean”
Claim · model-written summary
The RAPD results were reproducible across multiple isolations and three DNA isolation procedures.
- Taxon
- soybeanGlycine maxt0
- Outcome
- Results were reproducibly obtainede0
- Value
- three separate DNA isolation procedurese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Results were reproducibly obtained from multiple DNA isolations using three separate DNA isolation procedures involving either multiple seed or a single seed as the template source”
t0“soybean”