Abstract
Cyclin-dependent kinases (CDKs) form a conserved superfamily of eukaryotic serine-threonine protein kinases whose activity requires the binding of a cyclin protein. CDKs are involved in many aspects of cell biology and notably in the regulation of the cell cycle. Three cDNAs encoding a C-type CDK, and a member of each B-type CDK subfamily, were isolated from tomato (Lycopsersicon esculentum Mill.) and designated Lyces;CDKC;1 (accession no. AJ294903), Lyces; CDKB1;1 (accession no. AJ297916), and Lyces;CDKB2;1 (accession no. AJ297917). The predicted amino acid sequences displayed the characteristic PITAIRE (CDKC), PPTALRE (CDKB1), and PPTTLRE (CDKB2) motives in the cyclin-binding domain, clearly identifying the type of CDK. The accumulation of all transcripts was associated preferentially with dividing tissues in developing tomato fruit and vegetative organs. In contrast to that of CDKA and CDKBs, the transcription pattern of Lyces;CDKC;1 was shown to be independent of hormone and sugar supply in tomato cell suspension cultures and excised roots. This observation, together with the absence of a patchy expression profile in in situ hybridization experiments, suggests a non-cell cycle regulation of Lyces;CDKC;1. Using a two-hybrid assay, we showed that Lyces;CDKC;1 did not interact with mitotic and G1 cyclins. The role of plant CDKCs in the regulation of cell division and differentiation is discussed with regard to the known function of their animal counterparts.
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Claims 7
Claim · model-written summary
Three tomato CDK cDNAs were isolated and designated.
- Taxon
- tomato
Solanum lycopersicume0 - Outcome
- Three cDNAse0
- Value
- Threee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three cDNAs encoding a C-type CDK, and a member of each B-type CDK subfamily, were isolated from tomato (Lycopsersicon esculentum Mill.) and designated Lyces;CDKC;1 (accession no. AJ294903), Lyces; CDKB1;1 (accession no. AJ297916), and Lyces;CDKB2;1 (accession no. AJ297917)”
Claim · model-written summary
Predicted CDK sequences carried type-identifying PITAIRE, PPTALRE and PPTTLRE motifs.
- Outcome
- characteristic PITAIRE (CDKC), PPTALRE (CDKB1), and PPTTLRE (CDKB2) motivese0
Not reportedTaxon
Verbatim
e0“predicted amino acid sequences displayed the characteristic PITAIRE (CDKC), PPTALRE (CDKB1), and PPTTLRE (CDKB2) motives”
Claim · model-written summary
All three CDK transcripts preferentially accumulated in dividing developing fruit and vegetative tissues.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- dividing tissues in developing tomato fruit and vegetative organse0
- Outcome
- accumulation of all transcriptse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The accumulation of all transcripts was associated preferentially with dividing tissues in developing tomato fruit and vegetative organs”
Claim · model-written summary
Lyces;CDKC;1 transcription was independent of hormone and sugar supply in cell cultures and excised roots.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- hormone and sugar supplye0
- Gene
- Lyces;CDKC;1e0
- Outcome
- transcription patterne0
- Direction
- independente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcription pattern of Lyces;CDKC;1 was shown to be independent of hormone and sugar supply in tomato cell suspension cultures and excised roots”
Claim · model-written summary
Lyces;CDKC;1 lacked a patchy in situ expression pattern.
- Outcome
- absence of a patchy expression profilee0
Not reportedTaxon
Verbatim
e0“absence of a patchy expression profile in in situ hybridization experiments”
Claim · model-written summary
The expression results suggest non-cell-cycle regulation of Lyces;CDKC;1.
Not reportedTaxon
Verbatim
e0“suggests a non-cell cycle regulation of Lyces;CDKC;1”
Claim · model-written summary
Lyces;CDKC;1 did not interact with mitotic and G1 cyclins in a two-hybrid assay.
Not reportedTaxon
Verbatim
e0“Lyces;CDKC;1 did not interact with mitotic and G1 cyclins”
The same questions in other papers
class · molecular: expression · Solanum lycopersicum (tomato)Positive and negative results
6 papers in the same direction as this paper · 18 in the opposite direction
- positive
- positive
- positive
21 papers more in this group
See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.