Abstract
Embryogenic callus in Catharanthus roseus was initiated from hypocotyl on Murashige and Skoog's (MS) medium supplemented with 1.0-2.0 mg dm-3 of 2,4-dichlorophenoxyacetic acid (2,4-D) or chlorophenoxyacetic acid (CPA). Calli from other sources were non-embryogenic. Numerous somatic embryos were induced from primary callus on MS medium suplemented with naphthalene acetic acid (NAA) within two weeks of culture. Embryo proliferation was much faster on medium supplemented with 6-benzylaminopurine (BAP). After transfer to medium with gibberellic acid (GA3, 1.0 mg dm- 3) mature green embryos were developed and germinated well into plantlets on MS liquid medium supplemented with 0.5 mg dm-3 BAP. Later, embryos with cotyledonary leaves were subjected to different auxins treatments for the development of roots. Before transfer ex vitro, plantlets were cultivated on half strength MS medium containing 3 % sucrose and 0.5 mg dm-3 BAP for additional 2 weeks. Additionally, the effect of liquid medium has been evaluated at different morphogenetic stages.
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Claims 6
Claim · model-written summary
Hypocotyl explants on MS medium with 1.0–2.0 mg dm-3 2,4-D or CPA produced embryogenic callus.
- Taxon
- Catharanthus roseus
Catharanthus roseuse0 - Organ / stage
- hypocotyle0
- Treatment
- 1.0-2.0 mg dm-3 of 2,4-dichlorophenoxyacetic acid (2,4-D) or chlorophenoxyacetic acid (CPA)e0
- Outcome
- Embryogenic calluse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenic callus in Catharanthus roseus was initiated from hypocotyl on Murashige and Skoog's (MS) medium supplemented with 1.0-2.0 mg dm-3 of 2,4-dichlorophenoxyacetic acid (2,4-D) or chlorophenoxyacetic acid (CPA).”
Claim · model-written summary
Calli from other sources were nonembryogenic.
Not reportedTaxon
Verbatim
e0“Calli from other sources were non-embryogenic.”
Claim · model-written summary
NAA-supplemented MS medium induced numerous somatic embryos from primary callus within two weeks.
- Treatment
- MS medium suplemented with naphthalene acetic acid (NAA)e0
- Outcome
- somatic embryose0
- Direction
- inducede0
- Qualifier
- within two weeks of culturee0
Not reportedTaxonComparator
Verbatim
e0“Numerous somatic embryos were induced from primary callus on MS medium suplemented with naphthalene acetic acid (NAA) within two weeks of culture.”
Claim · model-written summary
BAP supplementation accelerated embryo proliferation.
- Treatment
- medium supplemented with 6-benzylaminopurine (BAP)e0
- Outcome
- Embryo proliferatione0
- Direction
- much fastere0
Not reportedTaxonComparator
Verbatim
e0“Embryo proliferation was much faster on medium supplemented with 6-benzylaminopurine (BAP).”
Claim · model-written summary
Transfer to GA3 medium produced mature green embryos.
- Treatment
- medium with gibberellic acid (GA3, 1.0 mg dm- 3)e0
- Outcome
- mature green embryose0
- Direction
- developede0
Not reportedTaxonComparator
Verbatim
e0“After transfer to medium with gibberellic acid (GA3, 1.0 mg dm- 3) mature green embryos were developed”
Claim · model-written summary
Mature embryos germinated into plantlets on liquid MS medium with 0.5 mg dm-3 BAP.
- Treatment
- MS liquid medium supplemented with 0.5 mg dm-3 BAPe0
- Outcome
- plantletse0
- Direction
- germinated welle0
Not reportedTaxonComparator
Verbatim
e0“germinated well into plantlets on MS liquid medium supplemented with 0.5 mg dm-3 BAP.”