Paper · 1999 · Communications in Soil Science and Plant Analysis

Effects of supplemental nitrogen on nitrogen‐assimilation enzymes, free amino nitrogen, soluble sugars, and crude protein of rice

Sonia R. Souza; Elvia M. L. M. Stark; Manlio S. Fernandes; et al.

11
claims
33
verbatim quotes
19
citations
2
research areas
—
Not open access

Abstract

Abstract An upland rice variety IAC‐47 was grown in a greenhouse to determine the effect of foliar nitrogen (N) supplementation during grain development on the activity of the N assimilation enzymes, nitrate reductase (NR) and glutamine synthetase (GS), on free amino‐N content and leaf soluble sugars, and on grain crude protein content. At 10 and 20 days after anthesis (DAA), the leaves were fertilized with a liquid fertilizer containing 32% N as 12.8% urea, 9.6% ammonium (NH4), and 9.6% nitrate (NO3) in increasing rates corresponding to 0,20+20, 40+40, and 60+60 kg N ha‐1. Leaves were collected twice (at 12 DAA and 14 DAA for GS activity, sugar and amino‐N content, and at 11 and 13 DAA for NRA) after each application of leaf N. The late foliar application of N increased significantly grain crude protein without a corresponding decrease in grain weight. The NR activity (NRA) increased after the foliar application of N. In the flag leaf, 60+60 kg N ha‐1 (21 DAA) resulted in higher NRA (20x over the control), while GS activity was smaller than the control. At 22 DAA there was an increase in GS activity in the flag leaf at 20+20 N level. However, the GS activity decreased as applied N levels increased. Also at the 20+20 level, there were increases in free amino‐N in the flag leaf and second leaf at the final harvest. Throughout the experiment, plants at the 60+60 N level had the lowest levels of soluble sugars. Increases in crude protein were highest at 40+40 N level (27.9%), followed by 60+60 (18.7%).

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Claims 11

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Late foliar N significantly increased grain crude protein.

Material
IAC‐47e0
Treatment
foliar application of Ne1
Outcome
grain crude proteine1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice”

✓ located verbatim in the source · title, characters 125–129 · show in text ↑
e0

“An upland rice variety IAC‐47 was grown in a greenhouse”

✓ located verbatim in the source · abstract, characters 9–64 · show in text ↑
e1

“The late foliar application of N increased significantly grain crude protein without a corresponding decrease in grain weight.”

✓ located verbatim in the source · abstract, characters 739–865 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Late foliar N did not correspondingly decrease grain weight.

Material
IAC‐47e0
Treatment
foliar application of Ne1
Outcome
grain weighte1
Direction
without a corresponding decreasee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice”

✓ located verbatim in the source · title, characters 125–129 · show in text ↑
e0

“An upland rice variety IAC‐47 was grown in a greenhouse”

✓ located verbatim in the source · abstract, characters 9–64 · show in text ↑
e1

“The late foliar application of N increased significantly grain crude protein without a corresponding decrease in grain weight.”

✓ located verbatim in the source · abstract, characters 739–865 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar N increased nitrate reductase activity.

Material
IAC‐47e0
Treatment
foliar application of Ne1
Outcome
NR activity (NRA)e1
Direction
increasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice”

✓ located verbatim in the source · title, characters 125–129 · show in text ↑
e0

“An upland rice variety IAC‐47 was grown in a greenhouse”

✓ located verbatim in the source · abstract, characters 9–64 · show in text ↑
e1

“The NR activity (NRA) increased after the foliar application of N.”

✓ located verbatim in the source · abstract, characters 866–932 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 21 DAA, 60+60 kg N ha‐1 gave 20 times higher flag-leaf NRA than control.

Material
IAC‐47e0
Treatment
60+60 kg N ha‐1e1
Outcome
NRAe1
Direction
highere1
Value
20xe1
Comparator
the controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice”

✓ located verbatim in the source · title, characters 125–129 · show in text ↑
e0

“An upland rice variety IAC‐47 was grown in a greenhouse”

✓ located verbatim in the source · abstract, characters 9–64 · show in text ↑
e1

“In the flag leaf, 60+60 kg N ha‐1 (21 DAA) resulted in higher NRA (20x over the control), while GS activity was smaller than the control.”

✓ located verbatim in the source · abstract, characters 933–1070 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

At 21 DAA, GS activity was below control under 60+60 kg N ha‐1.

Material
IAC‐47e0
Treatment
60+60 kg N ha‐1e1
Outcome
GS activitye1
Direction
smallere1
Comparator
the controle1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice”

✓ located verbatim in the source · title, characters 125–129 · show in text ↑
e0

“An upland rice variety IAC‐47 was grown in a greenhouse”

✓ located verbatim in the source · abstract, characters 9–64 · show in text ↑
e1

“In the flag leaf, 60+60 kg N ha‐1 (21 DAA) resulted in higher NRA (20x over the control), while GS activity was smaller than the control.”

✓ located verbatim in the source · abstract, characters 933–1070 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 22 DAA, 20+20 N increased flag-leaf GS activity.

Material
IAC‐47e0
Treatment
20+20 N levele1
Outcome
GS activitye1
Direction
increasee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice”

✓ located verbatim in the source · title, characters 125–129 · show in text ↑
e0

“An upland rice variety IAC‐47 was grown in a greenhouse”

✓ located verbatim in the source · abstract, characters 9–64 · show in text ↑
e1

“At 22 DAA there was an increase in GS activity in the flag leaf at 20+20 N level.”

✓ located verbatim in the source · abstract, characters 1071–1152 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

GS activity declined with increasing applied N levels.

Material
IAC‐47e0
Treatment
applied N levelse1
Outcome
GS activitye1
Direction
decreasede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice”

✓ located verbatim in the source · title, characters 125–129 · show in text ↑
e0

“An upland rice variety IAC‐47 was grown in a greenhouse”

✓ located verbatim in the source · abstract, characters 9–64 · show in text ↑
e1

“However, the GS activity decreased as applied N levels increased.”

✓ located verbatim in the source · abstract, characters 1153–1218 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

At 20+20 N, free amino-N increased in flag and second leaves at final harvest.

Material
IAC‐47e0
Treatment
20+20 levele1
Outcome
free amino‐Ne1
Direction
increasese1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice”

✓ located verbatim in the source · title, characters 125–129 · show in text ↑
e0

“An upland rice variety IAC‐47 was grown in a greenhouse”

✓ located verbatim in the source · abstract, characters 9–64 · show in text ↑
e1

“Also at the 20+20 level, there were increases in free amino‐N in the flag leaf and second leaf at the final harvest.”

✓ located verbatim in the source · abstract, characters 1219–1335 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The 60+60 N level had the lowest soluble sugar levels.

Material
IAC‐47e0
Treatment
60+60 N levele1
Outcome
soluble sugarse1
Direction
loweste1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice”

✓ located verbatim in the source · title, characters 125–129 · show in text ↑
e0

“An upland rice variety IAC‐47 was grown in a greenhouse”

✓ located verbatim in the source · abstract, characters 9–64 · show in text ↑
e1

“Throughout the experiment, plants at the 60+60 N level had the lowest levels of soluble sugars.”

✓ located verbatim in the source · abstract, characters 1336–1431 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Crude protein increased most at 40+40 N, by 27.9%.

Material
IAC‐47e0
Treatment
40+40 N levele1
Outcome
crude proteine1
Direction
higheste1
Value
27.9%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice”

✓ located verbatim in the source · title, characters 125–129 · show in text ↑
e0

“An upland rice variety IAC‐47 was grown in a greenhouse”

✓ located verbatim in the source · abstract, characters 9–64 · show in text ↑
e1

“Increases in crude protein were highest at 40+40 N level (27.9%), followed by 60+60 (18.7%).”

✓ located verbatim in the source · abstract, characters 1432–1524 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Crude protein increased by 18.7% at 60+60 N.

Material
IAC‐47e0
Treatment
60+60e1
Outcome
crude proteine1
Direction
Increasese1
Value
18.7%e1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Oryza sativa
Oryza sativa · Taxon · picture from its Wikimedia Commons categoryDesmanthus4food · CC BY-SA 3.0 us · Wikimedia Commons ↗

Verbatim

t0

“rice”

✓ located verbatim in the source · title, characters 125–129 · show in text ↑
e0

“An upland rice variety IAC‐47 was grown in a greenhouse”

✓ located verbatim in the source · abstract, characters 9–64 · show in text ↑
e1

“Increases in crude protein were highest at 40+40 N level (27.9%), followed by 60+60 (18.7%).”

✓ located verbatim in the source · abstract, characters 1432–1524 · show in text ↑

nitrogen · quality: protein · Oryza sativa (rice)Results differ

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nitrogen · molecular: metabolites · Oryza sativa (rice)Positive and negative results

121 papers: 86 positive, 35 negative or no effect

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nitrogen · yield · Oryza sativa (rice)Positive and negative results

2,045 papers: 1,803 positive, 242 negative or no effect

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.