Abstract
ABSTRACT Polyphenol oxidases (PPOs) present in mature wheat ( Triticum aestivum L.) kernels have been implicated in the undesirable darkening of cereal products such as Asian noodles. To accelerate the functional characterization of wheat PPOs and allow the identification of those PPO genes that are primarily involved in food biochemistry, several basic local alignment search tool (BLAST) searches of expressed sequence tag (EST) databases were performed using a known wheat PPO sequence as a search argument; identified ESTs were resequenced and aligned. Results from this study suggest the presence of at least six PPO genes in hexaploid wheat, falling into two clusters with three similar sequences each. Based on the tissues used for cDNA library preparation, three genes (all members of one cluster) are expressed during kernel development and may therefore influence cereal product quality; the remaining three genes (belonging to the second cluster) were isolated from nonkernel cDNA libraries and may not be expressed at high levels during grain development. Discovery of these genes represents an essential first step in the functional characterization of wheat PPOs.
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Claims 3
Claim · model-written summary
Hexaploid wheat appears to carry at least six PPO genes, in two clusters of three similar sequences each.
Verbatim
e1“suggest the presence of at least six PPO genes in hexaploid wheat”
Claim · model-written summary
Three PPO genes, all members of one cluster, are expressed during kernel development.
Verbatim
e1“suggest the presence of at least six PPO genes in hexaploid wheat”
e2“three genes (all members of one cluster) are expressed during kernel development”
Claim · model-written summary
The three PPO genes of the second cluster may not be expressed at high levels during grain development.
Verbatim
e1“suggest the presence of at least six PPO genes in hexaploid wheat”
e3“the remaining three genes (belonging to the second cluster) were isolated from nonkernel cDNA libraries and may not be expressed at high levels during grain development”