Paper · 1995 · Journal of Agricultural and Food Chemistry

Evolution of Trypsin Inhibitor Activity during Germination of Lentils

Juana Frias; Concepcion Diaz-Pollan; Cliff L. Hedley; et al.

10
claims
20
verbatim quotes
72
citations
1
research areas
—
Not open access

Abstract

The effect of light and seed rinsing during the germination of lentil seeds (Lens culinaris var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TLA) has been studied. TLA decreased progressively during germination, while hydration grade and light exposure had less influence on TIA. In general, there was no significant change in TLA after 3 days of germination, except when seeds were rinsed only once and the experiment was conducted in the dark, showing a 10% TLA decrease. Six-day germination produced TLA decreases from 7% to 18%. In this case, the number of rinses had no significant effect on the final TLA, but the activity of protease inhibitors was significantly higher in seeds illuminated for 6 h per day. Maximal TLA decreases were found in seeds germinated in darkness and rinsed daily. Under these optimum conditions, TLA first decreased slowly (12% loss of this activity after 6 days) followed by a faster decrease (up to 45% of control seeds values) after 10 days of germination.

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Claims 10

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Trypsin inhibitor activity decreased progressively during lentil germination.

Taxon
Lens culinarisLens culinarise0
Material
Magda-20e0
Treatment
during germinatione1
Outcome
TLAe1
Direction
decreased progressivelye1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The effect of light and seed rinsing during the germination of lentil seeds (Lens culinaris var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TLA) has been studied”

✓ located verbatim in the source · abstract, characters 0–172 · show in text ↑
e1

“TLA decreased progressively during germination, while hydration grade and light exposure had less influence on TIA”

✓ located verbatim in the source · abstract, characters 174–288 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

After 3 days of germination, trypsin inhibitor activity showed no significant change in lentil seeds, except when seeds were rinsed only once.

Taxon
Lens culinarisLens culinarise0
Treatment
after 3 days of germinatione2
Outcome
TLAe2
Direction
no significant changee2
Qualifier
except when seeds were rinsed only oncee2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The effect of light and seed rinsing during the germination of lentil seeds (Lens culinaris var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TLA) has been studied”

✓ located verbatim in the source · abstract, characters 0–172 · show in text ↑
e2

“In general, there was no significant change in TLA after 3 days of germination, except when seeds were rinsed only once and the experiment was conducted in the dark, showing a 10% TLA decrease”

✓ located verbatim in the source · abstract, characters 290–482 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Seeds rinsed only once and germinated in the dark showed a 10% decrease in trypsin inhibitor activity after 3 days.

Taxon
Lens culinarisLens culinarise0
Treatment
seeds were rinsed only oncee2
Outcome
TLAe2
Direction
decreasee2
Value
10%e2
Qualifier
after 3 days of germinatione2
Setting
in the darke2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The effect of light and seed rinsing during the germination of lentil seeds (Lens culinaris var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TLA) has been studied”

✓ located verbatim in the source · abstract, characters 0–172 · show in text ↑
e2

“In general, there was no significant change in TLA after 3 days of germination, except when seeds were rinsed only once and the experiment was conducted in the dark, showing a 10% TLA decrease”

✓ located verbatim in the source · abstract, characters 290–482 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Six days of germination decreased trypsin inhibitor activity by 7% to 18% in lentil seeds.

Taxon
Lens culinarisLens culinarise0
Treatment
Six-day germinatione3
Outcome
TLAe3
Direction
decreasese3
Value
7% to 18%e3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The effect of light and seed rinsing during the germination of lentil seeds (Lens culinaris var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TLA) has been studied”

✓ located verbatim in the source · abstract, characters 0–172 · show in text ↑
e3

“Six-day germination produced TLA decreases from 7% to 18%”

✓ located verbatim in the source · abstract, characters 484–541 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

The number of rinses had no significant effect on the final trypsin inhibitor activity of lentil seeds after six days of germination.

Taxon
Lens culinarisLens culinarise0
Treatment
the number of rinsese4
Outcome
final TLAe4
Direction
no significant effecte4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The effect of light and seed rinsing during the germination of lentil seeds (Lens culinaris var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TLA) has been studied”

✓ located verbatim in the source · abstract, characters 0–172 · show in text ↑
e4

“In this case, the number of rinses had no significant effect on the final TLA, but the activity of protease inhibitors was significantly higher in seeds illuminated for 6 h per day”

✓ located verbatim in the source · abstract, characters 543–723 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Seeds illuminated for 6 h per day had significantly higher protease inhibitor activity during lentil germination.

Taxon
Lens culinarisLens culinarise0
Treatment
illuminated for 6 h per daye4
Outcome
activity of protease inhibitorse4
Direction
significantly highere4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The effect of light and seed rinsing during the germination of lentil seeds (Lens culinaris var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TLA) has been studied”

✓ located verbatim in the source · abstract, characters 0–172 · show in text ↑
e4

“In this case, the number of rinses had no significant effect on the final TLA, but the activity of protease inhibitors was significantly higher in seeds illuminated for 6 h per day”

✓ located verbatim in the source · abstract, characters 543–723 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Maximal decreases in trypsin inhibitor activity were found in lentil seeds germinated in darkness and rinsed daily.

Taxon
Lens culinarisLens culinarise0
Treatment
germinated in darkness and rinsed dailye5
Outcome
TLAe5
Direction
decreasese5
Qualifier
Maximale5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The effect of light and seed rinsing during the germination of lentil seeds (Lens culinaris var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TLA) has been studied”

✓ located verbatim in the source · abstract, characters 0–172 · show in text ↑
e5

“Maximal TLA decreases were found in seeds germinated in darkness and rinsed daily”

✓ located verbatim in the source · abstract, characters 725–806 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Under optimum conditions, trypsin inhibitor activity first decreased slowly, with a 12% loss after 6 days of germination.

Taxon
Lens culinarisLens culinarise0
Treatment
optimum conditionse6
Outcome
TLAe6
Direction
decreased slowlye6
Value
12%e6
Qualifier
after 6 dayse6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The effect of light and seed rinsing during the germination of lentil seeds (Lens culinaris var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TLA) has been studied”

✓ located verbatim in the source · abstract, characters 0–172 · show in text ↑
e6

“Under these optimum conditions, TLA first decreased slowly (12% loss of this activity after 6 days) followed by a faster decrease (up to 45% of control seeds values) after 10 days of germination”

✓ located verbatim in the source · abstract, characters 808–1002 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Under optimum conditions, trypsin inhibitor activity then decreased faster, by up to 45% of control seed values after 10 days of germination.

Taxon
Lens culinarisLens culinarise0
Treatment
optimum conditionse6
Outcome
TLAe6
Direction
faster decreasee6
Value
up to 45%e6
Qualifier
after 10 dayse6
Comparator
control seedse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The effect of light and seed rinsing during the germination of lentil seeds (Lens culinaris var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TLA) has been studied”

✓ located verbatim in the source · abstract, characters 0–172 · show in text ↑
e6

“Under these optimum conditions, TLA first decreased slowly (12% loss of this activity after 6 days) followed by a faster decrease (up to 45% of control seeds values) after 10 days of germination”

✓ located verbatim in the source · abstract, characters 808–1002 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Hydration grade and light exposure had less influence on trypsin inhibitor activity than germination during lentil germination.

Taxon
Lens culinarisLens culinarise0
Treatment
hydration grade and light exposuree1
Outcome
TIAe1
Direction
less influencee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lens culinaris
Lens culinaris · TaxonUser:Justinc · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The effect of light and seed rinsing during the germination of lentil seeds (Lens culinaris var. Vulgaris cv. Magda-20) on trypsin inhibitor activity (TLA) has been studied”

✓ located verbatim in the source · abstract, characters 0–172 · show in text ↑
e1

“TLA decreased progressively during germination, while hydration grade and light exposure had less influence on TIA”

✓ located verbatim in the source · abstract, characters 174–288 · show in text ↑