Abstract
Symbiotic N(2) fixation of legume crops is highly sensitive to drought, which results in a dramatic drop of N accumulation and yield. The symbiosis between soybean (Glycine max) and Bradyrhizobium japonicum, because of its extreme sensitivity to drought, was chosen as a model to analyse the response to drought stress at a molecular level. The mRNA differential display technique was performed to isolate cDNA markers differentially expressed in well-watered [100% of N(2) fixation capacity (NFC)] and drought-stressed nodules (40% NFC). One gene noted, G93, appeared strongly down-regulated by drought and fully recovered after rehydration. In situ hybridization showed that G93 transcripts were localized in N(2)-fixing cells of mature nodules, indicating that G93 could be considered as a late nodulin. However, G93 expression was not directly correlated to N(2) fixation but mainly responded to osmotic stress. Other stresses that lead to decrease of N(2) fixation did not affect G93 expression. Sequence analyses showed that G93 presented a strong homology with two soybean expressed sequence tags (ESTs) and with the ZR1 protein of Medicago sativa. Putative roles of this nodulin in adaptation of soybean nodule to osmotic stress are proposed.
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Claims 5
Claim · model-written summary
Drought strongly down-regulated G93 expression, which recovered after rehydration.
- Taxon
- Glycine max
Glycine maxt0 - Treatment
- droughte0
- Interactor
- Bradyrhizobium japonicum
Bradyrhizobium japonicumt0symbiont - Gene
- G93e0
- Outcome
- G93e0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One gene noted, G93, appeared strongly down-regulated by drought and fully recovered after rehydration.”
t0“Glycine max/Bradyrhizobium japonicum nodule”
Claim · model-written summary
G93 transcripts localized in nitrogen-fixing cells of mature nodules.
- Taxon
- Glycine maxGlycine maxt0
- Organ / stage
- mature nodulese0
- Interactor
- Bradyrhizobium japonicumBradyrhizobium japonicumt0symbiont
- Gene
- G93e0
- Outcome
- G93 transcripts were localized in N(2)-fixing cellse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“G93 transcripts were localized in N(2)-fixing cells of mature nodules”
t0“Glycine max/Bradyrhizobium japonicum nodule”
Claim · model-written summary
G93 expression responded mainly to osmotic stress rather than directly tracking nitrogen fixation.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- osmotic stresse0
- Interactor
- Bradyrhizobium japonicumBradyrhizobium japonicumt0symbiont
- Gene
- G93e0
- Outcome
- G93 expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“G93 expression was not directly correlated to N(2) fixation but mainly responded to osmotic stress”
t0“Glycine max/Bradyrhizobium japonicum nodule”
Claim · model-written summary
Other stresses reducing nitrogen fixation did not affect G93 expression.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- Other stressese0
- Interactor
- Bradyrhizobium japonicumBradyrhizobium japonicumt0symbiont
- Gene
- G93e0
- Outcome
- G93 expressione0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other stresses that lead to decrease of N(2) fixation did not affect G93 expression.”
t0“Glycine max/Bradyrhizobium japonicum nodule”
Claim · model-written summary
G93 showed strong sequence homology to two soybean ESTs and Medicago sativa ZR1 protein.
- Taxon
- Glycine maxGlycine maxt0
- Interactor
- Bradyrhizobium japonicumBradyrhizobium japonicumt0symbiont
- Gene
- G93e0
- Outcome
- strong homologye0
- Comparator
- two soybean expressed sequence tags (ESTs) and with the ZR1 protein of Medicago sativae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“G93 presented a strong homology with two soybean expressed sequence tags (ESTs) and with the ZR1 protein of Medicago sativa”
t0“Glycine max/Bradyrhizobium japonicum nodule”
The same questions in other papers
drought · Bradyrhizobium japonicum · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- negative
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.