Paper · 2010 · Crop Science

Genetic Markers and Leaf Rust Resistance of the Wheat Gene Lr32

J. Thomas; S. Nilmalgoda; C. Hiebert; et al.

6
claims
12
verbatim quotes
33
citations
4
research areas
—
Not open access

Abstract

Leaf rust (caused by Puccinia triticina Eriks.) is a worldwide and yield‐limiting disease of common wheat ( Triticum aestivum L.). The gene Lr32 , which confers seedling resistance to leaf rust, was previously transferred from Aegilops tauschii Coss. (RL5497‐1) to chromosome 3DS of common wheat. Without exception, Lr32 conferred resistance to 616 diverse isolates of leaf rust sampled from Canadian wheat fields over the past decade (2000 to 2009). Marker alleles specific to chromosome 3DS of RL5497 were found in Lr32 backcross lines of both Katepwa (BW196R) and Thatcher (RL6086). These introgressions from A. tauschii had no adverse effects on either agronomic performance or end use quality. A map based on 338 doubled haploid lines (DHL) from the cross of BW196R × Thatcher showed that Lr32 was slightly distal (0.6 cM) to two simple sequence repeat (SSR) loci wmc43 and barc135, which cosegregated on chromosome 3DS. A survey of the fragments amplified in wheats of diverse origin showed that both wmc43 and barc135 were cross‐applicable as markers for stacking Lr32 with other broadly effective leaf rust resistance genes.

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Claims 6

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Without exception, Lr32 conferred resistance to 616 diverse leaf rust isolates sampled from Canadian wheat fields between 2000 and 2009.

Interactor
leaf ruste0pathogen
Gene
Lr32t0
Outcome
resistancee0
Value
616 diverse isolatese0
Setting
Canadian wheat fieldse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat Gene Lr32”

✓ located verbatim in the source · title, characters 48–63 · show in text ↑
e0

“Without exception, Lr32 conferred resistance to 616 diverse isolates of leaf rust sampled from Canadian wheat fields over the past decade (2000 to 2009)”

✓ located verbatim in the source · abstract, characters 297–449 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Marker alleles specific to chromosome 3DS of RL5497 were found in Lr32 backcross lines of both Katepwa (BW196R) and Thatcher (RL6086).

Material
Katepwa (BW196R) and Thatcher (RL6086)e0
Gene
Lr32t0
Outcome
Marker alleles specific to chromosome 3DS of RL5497e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat Gene Lr32”

✓ located verbatim in the source · title, characters 48–63 · show in text ↑
e0

“Marker alleles specific to chromosome 3DS of RL5497 were found in Lr32 backcross lines of both Katepwa (BW196R) and Thatcher (RL6086)”

✓ located verbatim in the source · abstract, characters 451–584 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

The Aegilops tauschii introgressions had no adverse effects on agronomic performance or end use quality of the wheat backcross lines.

Treatment
introgressions from A. tauschiie0
Outcome
agronomic performance or end use qualitye0
Direction
no adverse effectse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat Gene Lr32”

✓ located verbatim in the source · title, characters 48–63 · show in text ↑
e0

“These introgressions from A. tauschii had no adverse effects on either agronomic performance or end use quality”

✓ located verbatim in the source · abstract, characters 586–697 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

In the mapping population of 338 doubled haploid lines, Lr32 was slightly distal (0.6 cM) to the SSR loci wmc43 and barc135.

Gene
wmc43 and barc135e0
Outcome
slightly distale0
Value
0.6 cMe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat Gene Lr32”

✓ located verbatim in the source · title, characters 48–63 · show in text ↑
e0

“Lr32 was slightly distal (0.6 cM) to two simple sequence repeat (SSR) loci wmc43 and barc135”

✓ located verbatim in the source · abstract, characters 794–886 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The SSR loci wmc43 and barc135 cosegregated on chromosome 3DS.

Gene
wmc43 and barc135e0
Outcome
cosegregated on chromosome 3DSe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat Gene Lr32”

✓ located verbatim in the source · title, characters 48–63 · show in text ↑
e0

“wmc43 and barc135, which cosegregated on chromosome 3DS”

✓ located verbatim in the source · abstract, characters 869–924 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Both wmc43 and barc135 were cross-applicable as markers in diverse wheats for stacking Lr32 with other leaf rust resistance genes.

Gene
wmc43 and barc135e0
Outcome
cross‐applicable as markerse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“Wheat Gene Lr32”

✓ located verbatim in the source · title, characters 48–63 · show in text ↑
e0

“both wmc43 and barc135 were cross‐applicable as markers”

✓ located verbatim in the source · abstract, characters 1002–1057 · show in text ↑

transgenic · quality: general · Triticum aestivum (wheat)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.